弓形虫卵囊感染小鼠的急性期与慢性期的脑组织蛋白质组变化

doi:10.11843/j.issn.0366-6964.2022.02.022

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (2): 556-566.doi: 10.11843/j.issn.0366-6964.2022.02.022

弓形虫卵囊感染小鼠的急性期与慢性期的脑组织蛋白质组变化

付明^1,2, 贺君君², 朱兴全^3,4, 丛伟^1,2*

1. 山东大学海洋学院, 威海 264209;
2. 中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室, 兰州 730046;
3. 山西农业大学动物医学学院, 太谷 030801;
4. 云南农业大学动物医学院, 云南省高校兽医公共卫生重点实验室, 昆明 650201

收稿日期:2021-05-07 出版日期:2022-02-23 发布日期:2022-03-02
通讯作者: 丛伟,主要从事人兽共患寄生虫病研究,E-mail:messicw@163.com
作者简介:付明(1996-),男,河北石家庄人,硕士生,主要从事弓形虫与宿主互作研究,E-mail:fuming_hbny2016@163.com
基金资助:
山东大学（威海）青年学者未来计划项目（20820211009）；山东省博士后创新项目（202001005）；山东省重点研发计划（公益类项目）（2019GSF108135）；山西省“1331工程”项目（20211331-13）；云南省专家工作站项目（202005AF150041）

Proteomic Analysis of Changes in the Mouse Brain Tissue Infected with Toxoplasma gondii Oocysts during the Acute and Chronic Stage

FU Ming^1,2, HE Junjun², ZHU Xingquan^3,4, CONG Wei^1,2*

1. Marine College, Shandong University, Weihai 264209, China;
2. State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;
3. College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
4. Key Laboratory of Veterinary Public Health of Yunnan Province, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China

Received:2021-05-07 Online:2022-02-23 Published:2022-03-02

摘要/Abstract

摘要： 弓形虫是一种呈世界性分布的机会性致病原虫，可引起致命性脑炎。人弓形虫病急性感染往往与弓形虫卵囊污染密切相关。探究弓形虫卵囊感染对宿主的致病机制和防控弓形虫病具有重要意义。因此，本研究利用iTRAQ技术，结合2D-LC-MS/MS分析弓形虫PRU虫株卵囊急、慢性感染小鼠后，其脑组织蛋白质的差异表达情况。结果表明，在急性感染期和慢性感染期小鼠脑组织中分别鉴定到85和51个差异表达的蛋白，而慢性感染期与急性感染期相比有114个蛋白差异表达。对差异表达蛋白进行功能分析发现其主要涉及到免疫、代谢过程等通路。本研究结果为进一步阐明弓形虫卵囊急慢性感染引起宿主脑组织损伤及致病机制提供了重要的数据。

关键词: 弓形虫, iTRAQ, 脑组织, 蛋白质组学, 生物信息学分析

Abstract: Toxoplasma gondii is an opportunistic pathogen with a worldwide distribution, which can cause fatal encephalitis. Human acute toxoplasmosis is closely related to the contamination of T. gondii oocysts. To explore the pathogenic mechanism of T. gondii oocyst infection on the host and to prevent and control toxoplasmosis, we used iTRAQ to analyze the differential expression of proteins in mouse brain tissue after acute and chronic infection with T. gondii PRU strain oocysts. Compared with the control group, 85 and 51 differentially expressed proteins were identified in the acute and chronic infection, respectively, while 114 proteins were differentially expressed in the chronic versus acute infection. The functional analysis revealed that these differentially expressed proteins are mainly involved in pathways such as immune and metabolic processes. The results of our study provide important data for elucidating the host brain tissue damage and pathogenic mechanism caused by T. gondii oocysts in the acute and chronic infection.

Key words: Toxoplasma gondii, iTRAQ, brain, proteomics, bioinformatic analysis

中图分类号:

S855.9

付明, 贺君君, 朱兴全, 丛伟. 弓形虫卵囊感染小鼠的急性期与慢性期的脑组织蛋白质组变化[J]. 畜牧兽医学报, 2022, 53(2): 556-566.

FU Ming, HE Junjun, ZHU Xingquan, CONG Wei. Proteomic Analysis of Changes in the Mouse Brain Tissue Infected with Toxoplasma gondii Oocysts during the Acute and Chronic Stage[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 556-566.

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弓形虫卵囊感染小鼠的急性期与慢性期的脑组织蛋白质组变化

Proteomic Analysis of Changes in the Mouse Brain Tissue Infected with Toxoplasma gondii Oocysts during the Acute and Chronic Stage

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