我国部分地区羊芽囊原虫流行病学调查

doi:10.11843/j.issn.0366-6964.2021.04.021

摘要/Abstract

摘要： 芽囊原虫（Blastocystis spp.）是人和动物肠道中最常见的原生生物之一，能引起人和动物的肠道疾病和皮肤疾病，且有人兽共患基因亚型。为了解不同地区羊芽囊原虫的感染情况，对来自7个省、区的704份羊粪便样品，基于核糖体小亚基基因（SSU rDNA）位点进行分子流行病学调查，并首次对羊源人兽共患亚型ST3、ST4进行多位点序列分型。结果显示，芽囊原虫总感染率为13.35%（94/704），不同地区、不同品种间感染率差异极显著。芽囊原虫序列分析共发现5个亚型（ST3、ST4、ST5、ST10和ST14），其中，ST3、ST4和ST5为人兽共患基因亚型，MLST分析ST3为Allele 38且在2个基因位点发生了碱基突变。ST4包括Allele 42、Allele 92和Allele 94 3种等位基因。本次调查表明羊芽囊原虫感染普遍，病原存在人兽共患亚型且具遗传多样性，应给予足够重视。

关键词: 羊, 芽囊原虫, SSU rDNA, 亚型, 等位基因, MLST

Abstract: Blastocystis is one of the most common protozoan in human and animal intestines. It can cause intestinal and skin diseases in human and animals. In order to understand the infection status of Blastocystis from sheep and goats in different regions, 704 fecal samples from 7 provinces and regions were investigated by molecular epidemiology based on the SSU rDNA locus, and Multilocus-sequence typing was performed for the first time on ST3 and ST4 of Blastocystis derived from sheep and goats. The results showed that, the total infection rate of Blastocystis was 13.35% (94/704), and the infection rate was significantly different among different regions and breeds. There were 5 subtypes (ST3, ST4, ST5, ST10 and ST14), among which, ST3, ST4 and ST5 were zoonotic subtypes. MLST analysis showed that ST3 was Allele 38, and there were base mutations at 2 gene loci. ST4 included Allele 42, Allele 92 and Allele 94. The results indicated that, the infection of Blastocystis was common in sheep and goats, the zoonotic subgenotype and high genetic diversity of Blastocystis were found too，so more attention should be paid on its prevention and control.

Key words: sheep, Blastocystis, SSU rDNA, subtype, allele, MLST

中图分类号:

刘林科, 王朋林, 菅忆晨, 李世杰, 王占铭, 王荣军, 张龙现, 宁长申, 菅复春. 我国部分地区羊芽囊原虫流行病学调查[J]. 畜牧兽医学报, 2021, 52(4): 1061-1068.

LIU Linke, WANG Penglin, JIAN Yichen, LI Shijie, WANG Zhanming, WANG Rongjun, ZHANG Longxian, NING Changshen, JIAN Fuchun. Epidemiological Investigation of Blastocystis from Sheep and Goats in Some Areas of China[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1061-1068.

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