牦牛源干燥棒状杆菌的分离与鉴定

doi:10.11843/j.issn.0366-6964.2019.01.026

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (1): 227-232.doi: 10.11843/j.issn.0366-6964.2019.01.026

• 研究简报 • 上一篇

牦牛源干燥棒状杆菌的分离与鉴定

温峰琴¹, 项海涛¹, 郝宝成², 邢小勇¹, 包世俊¹, 胡永浩^1*

1. 甘肃农业大学动物医学院, 兰州 730070;
2. 中国农业科学院兰州畜牧与兽药研究所, 兰州 730050

收稿日期:2018-03-07 出版日期:2019-01-23 发布日期:2019-01-23
通讯作者: 胡永浩,主要从事畜禽传染病及病原分子生物学、动物防疫与检疫研究,E-mail:yhh0817@126.com
作者简介:温峰琴(1980-),女,甘肃崇信人,讲师,硕士,主要从事动物传染病学及病原分子生物学研究,E-mail:46292083@qq.com
基金资助:
盛彤笙基金（GSAU-STS-1521）；甘肃农业大学动物医学院教研产学支持计划（JYCX-KX014）

Isolation and Identification of Corynebacterium Xerosis Isolated from Yak Liver

WEN Fengqin¹, XIANG Haitao¹, HAO Baocheng², XING Xiaoyong¹, BAO Shijun¹, HU Yonghao^1*

1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;
2. Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China

Received:2018-03-07 Online:2019-01-23 Published:2019-01-23

摘要/Abstract

摘要：

一只牦牛屠宰后肝有干酪样结节，为了探究其形成原因，本研究对该牦牛肝进行细菌的分离培养，对所分离可疑致病菌的16S rRNA以及rpoB基因序列进行扩增和序列分析，并用Vitek 2全自动微生物鉴定仪进行生化表型的鉴定，用K-B法进行药敏试验。结果显示，经细菌分离培养得到一株革兰阳性、棒状、可疑致病菌；其16S rRNA扩增片段大小1 483 bp，GenBank序列编号为MH000696，该菌的16S rRNA与干燥棒状杆菌GD34株（KF928790.1）等的相似性最高，序列一致性为99%，系统进化树显示，本菌与干燥棒状杆菌在同一进化分支上；rpoB基因扩增片段大小434 bp，GenBank序列编号为MH006608；Vitek 2全自动微生物鉴定仪鉴定为变异库克菌，可信度为93%。该菌对妥布霉素、氨曲南、头孢哌酮、米诺环素等多种药物高度敏感。综上所述，分离的细菌为干燥棒状杆菌，但基因序列，如rpoB等以及生化表型均存在一定程度的变异，本研究为兽医临床上该菌的分离和鉴定提供了依据，且为该菌的致病性研究奠定了基础。

Abstract:

To investigate the formation reasons of caseous nodules in the liver of a yak, bacteria were isolated and cultured from the liver, and the 16S rRNA and rpoB gene sequences of isolated bacteria were amplified and sequenced. The biochemical phenotypes were identified by Vitek 2 automatic microbiology analyzer and drug susceptibility test was performed by K-B method. Results were as follows:A Gram-positive, rod-shaped and suspected pathogen was isolated. The amplification lengths of 16S rRNA and rpoB were 1 483 and 434 bp, while the accession number were MH000696 and MH006608, respectively. The similarity of 16S rRNA of clinical isolate to Corynebacterium xerosis was as high as 99% and phylogenetic tree showed it lied in the same branch with Corynebacterim xerosis. The results of Vitek 2 fully automatic Microbial Identification System showed that it was identified as Kocuria varians, with the probability of 93%. The bacteria was highly sensitive to tobramycin, aztreonam, cefoperazone, minocycline and other medicines. In summary, the isolated bacteria is Corynebacterium xerosis. But the gene sequence, such as rpoB, and biochemical property have a certain degree of mutations. This study provides reference for the isolation and identification in the veterinary clinic, and is the foundation of pathogenesis research of this bacteria.

中图分类号:

S855.1

温峰琴, 项海涛, 郝宝成, 邢小勇, 包世俊, 胡永浩. 牦牛源干燥棒状杆菌的分离与鉴定[J]. 畜牧兽医学报, 2019, 50(1): 227-232.

WEN Fengqin, XIANG Haitao, HAO Baocheng, XING Xiaoyong, BAO Shijun, HU Yonghao. Isolation and Identification of Corynebacterium Xerosis Isolated from Yak Liver[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 227-232.

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牦牛源干燥棒状杆菌的分离与鉴定

Isolation and Identification of Corynebacterium Xerosis Isolated from Yak Liver

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