鸡胚心脏组织转录组数据鉴定雪域白鸡高原低氧适应性关键基因

doi:10.11843/j.issn.0366-6964.2023.10.014

摘要/Abstract

摘要： 旨在通过分析白来航（White Leghorn，WL）和雪域白鸡（Xueyu White chicken，XYW）在低氧条件下孵化时胚胎心脏组织的基因表达差异，挖掘鸡胚低氧适应候选基因。本研究在高海拔环境孵化雪域白鸡和白来航鸡种蛋，采集孵化第16天胚胎心脏组织，提取RNA，进行转录组测序（RNA-seq），筛选差异表达基因（DEGs），并对其进行荧光定量PCR （qRT-PCR）验证、功能富集分析和构建转录因子-靶基因调控网络，鉴定与雪域白鸡低氧适应相关的候选基因。在雪域白鸡和白来航鸡的心脏组织之间筛选到253个DEGs，随机选择5个DEGs进行qRT-PCR验证，表达趋势与测序结果一致。功能富集分析表明，DEGs主要涉及心血管发育和心脏功能相关的生物学过程和信号通路。将DEGs和已知的鸡转录因子对比，筛选到FOXP2和HOXA2，参与调控血管生成、心肌收缩等，对雪域白鸡胚胎低氧适应起关键作用。本研究通过鸡胚心脏组织转录组数据分析，鉴定到TENM2、NOG、SMOC1、CCBE1等鸡胚高原低氧适应候选基因，为解析雪域白鸡高原低氧适应分子机制提供理论依据。

关键词: 雪域白鸡, 白来航鸡, 低氧适应, 心脏, 转录组

Abstract: The aim of this study was to identify candidate genes for hypoxia adaptation in chicken embryos by analyzing the gene expression changes in embryonic heart tissues of White Leghorn (WL) and Xueyu White (XYW) chickens incubated under hypoxic conditions. The eggs were incubated at high altitude. The heart tissues were collected from embryos at day 16 of incubation, and the RNAs were extracted for RNA sequencing (RNA-seq). Differently expressed genes (DEGs) were screened, which were validated using fluorescence quantitative PCR (qRT-PCR). The functional enrichment analysis and a transcription factor-target gene regulatory network was constructed to further screen candidate genes related to hypoxia adaptation in XYW. The 253 DEGs were screened in heart tissues between XYW and WL, and 5 DEGs were randomly selected for qRT-PCR validation. Their expression trend was consistent with the sequencing results. Enrichment analysis showed that DEGs were mainly enriched in biological processes and signaling pathways related to cardiovascular development and cardiac function. Comparing DEGs with known chicken transcription factors, FOXP2 and HOXA2 were screened, which regulated angiogenesis, myocardial contraction and other functions, and played a key role in hypoxia adaptation in XYW embryos. Through the analysis of transcriptomic data, TENM2, NOG, SMOC1, CCBE1 and other candidate genes were identified for plateau hypoxia adaptation in chicken embryos, which provide a theoretical basis for analyzing the molecular mechanism of plateau hypoxia adaptation in XYW.

Key words: Xueyu White chicken, White Leghorn chicken, hypoxia adaptation, heart, transcriptome

中图分类号:

S831.2

陈雪娇, 刘会杰, 臧蕾, 冯静, 鹏达, 张浩. 鸡胚心脏组织转录组数据鉴定雪域白鸡高原低氧适应性关键基因[J]. 畜牧兽医学报, 2023, 54(10): 4154-4163.

CHEN Xuejiao, LIU Huijie, ZANG Lei, FENG Jing, PENG Da, ZHANG Hao. Transcriptome Data from Chicken Embryo Heart Tissue Identified Key Genes for Altitude Hypoxia Adaptation in Xueyu White Chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4154-4163.

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