基因Ⅶ型新城疫病毒对鸭和SPF鸡致病性比较研究

doi:10.11843/j.issn.0366-6964.2013.12.018

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (12): 1982-1988.doi: 10.11843/j.issn.0366-6964.2013.12.018

基因Ⅶ型新城疫病毒对鸭和SPF鸡致病性比较研究

伊惠^1,2，胡北侠²，杨少华²，许传田²，黄庆华²，张琳²，张秀美²，王莉莉²，张伟^1,2，袁朋^1,2，李建亮¹，崔言顺^1*

(1.山东农业大学动物科技学院，泰安 271018；2.山东省农业科学院畜牧兽医研究所，山东省畜禽疫病防治与繁育重点实验室，济南 250100)

收稿日期:2013-06-25 出版日期:2013-12-23 发布日期:2013-12-23
通讯作者: 崔言顺，教授，E-mail:yscui@sdau.edu.cn
作者简介:伊惠（1987-），女，山东肥城人，在读硕士，主要从事禽病防治研究，E-mail: YHLZ2008@163.com
基金资助:
公益性行业（农业）科研专项经费项目(201003012；201303033)；现代农业产业技术体系建设专项资金（CARS-42-Z12）

Pathogenicity Comparison of Newcastle Disease Virus Isolates of Genotype Ⅶ in Ducks and SPF Chickens

YI Hui^1,2, HU Bei-xia², YANG Shao-hua² , XU Chuan-tian², HUANG Qing-hua², ZHANG Lin², ZHANG Xiu-mei², WANG Li-li², ZHANG Wei^1,2,YUAN Peng^1,2, LI Jian-liang¹, CUI Yan-shun^1*

(1.College of Animal Science, Shandong Agricultural University, Tai′an 271018, China; 2. Shandong Key Lab of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China)

Received:2013-06-25 Online:2013-12-23 Published:2013-12-23

摘要/Abstract

摘要：

旨在研究基因Ⅶ型新城疫病毒（NDV）对鸭的致病性，以明确鸭在NDV流行病学中的作用。选取2株鸭源基因Ⅶ型NDV流行株，以0.2和0.5 mL剂量分别通过滴鼻或肌肉注射对商品鸭进行人工感染试验，同时用0.1 mL剂量通过滴鼻感染SPF鸡。SPF鸡用2株NDV攻毒后表现气喘、流涎和排绿色稀粪等症状，并于3～6 d 100％发病死亡，而鸭用2株NDV攻毒后未见明显发病死亡。SPF鸡攻毒后喉头、泄殖腔棉拭和内脏组织病毒分离率明显高于试验鸭。病理组织学检查发现SPF鸡感染NDV后可见非化脓性脑炎、间质性肾炎和肺炎、明显的消化道和呼吸道炎症，而试验鸭仅呼吸道和消化道组织表现轻度炎症。本研究结果表明2株基因Ⅶ型NDV强毒对鸭无明显致病性，但鸭感染后NDV强毒后可以通过喉头或泄殖腔排毒，因此必须采取有效措施以防止NDV在鸡群和鸭群之间相互传播。

Abstract:

Two NDV strains isolated in ducks, Duck/China/SD03/2009 and JSD0812, were used to evaluate the pathogenicity of genotype Ⅶ NDV in ducks and the potential role of ducks in the epidemiology of NDV. Eighty 20-day-old ducks and sixteen 50-day-old SPF chickens were inoculated with Duck/China/SD03/2009 or JSD0812. Ducks of different groups were challenged with NDV intranasally or intramuscularly at the dose of 0.2 and 0.5 mL, respectively. The SPF chickens were infected with NDV intranasally at a dose of 0.1 mL. All of chickens inoculated with NDV had depression, gasping, oral discharges, and greenish-white soft feces and died in 6 days. However, ducks remained clinically normal throughout the study. Viruses were detected more frequently in the swabs and tissues of chickens than ducks. Gross and histologic lesion patterns supported the different clinical outcome. Ducks had slight inflammation mainly in respiratory and digestive tracts, whereas slight nonpurulent encephalitis, tubulointerstitial nephritis, and serious inflammation in respiratory and digestive tracts were detected in chickens. These results showed that both of the NDV strains are virulent in chickens whereas have no obvious pathogenicity in ducks. However, some measures should be taken to prevent virus transmission between ducks and chickens as viruses were detected in swabs of ducks infected with virulent NDV strains.

中图分类号:

S852.659.5

伊惠，胡北侠，杨少华，许传田，黄庆华，张琳，张秀美，王莉莉，张伟，袁朋，李建亮，崔言顺. 基因Ⅶ型新城疫病毒对鸭和SPF鸡致病性比较研究[J]. 畜牧兽医学报, 2013, 44(12): 1982-1988.

YI Hui, HU Bei-xia, YANG Shao-hua,XU Chuan-tian, HUANG Qing-hua, ZHANG Lin, ZHANG Xiu-mei, WANG Li-li, ZHANG Wei,YUAN Peng, LI Jian-liang, CUI Yan-shun. Pathogenicity Comparison of Newcastle Disease Virus Isolates of Genotype Ⅶ in Ducks and SPF Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1982-1988.

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基因Ⅶ型新城疫病毒对鸭和SPF鸡致病性比较研究

Pathogenicity Comparison of Newcastle Disease Virus Isolates of Genotype Ⅶ in Ducks and SPF Chickens

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