Abstract:

In order to find the different expression genes in longissimus muscle of Min pig and Yorkshire pig. In this study, the digital gene expression(DGE) profile in longissimus dorsi muscle of Min pig (fat) and Yorkshire pig (lean) were built by high-throughout sequencing technology, the different expression genes were screened and annotation. Significant enrichment analysis of GO and Pathway were done. The results showed that more than 5 000 000 clean tags were obtained from Min pig and Yorkshire pig, the tags whose copy number were greater than 100 occupied the most percentage, Min pig and Yorkshire pig were 88.62% and 84.62%, respectively. Completely blast to the sense chain and one tag only blast to one gene, Min pig and Yorkshire pig were 2 351 788 and 2 388 175, respectively. Min pig compared to Yorkshire pig, different multiples more than two times were 1 098, among them, 44 were up-regulated genes and 1 054 were down-regulated genes, 11 genes expressed only in Min pig, 256 genes expressed only in Yorkshire pig. Different expression genes included some genes and transcripts related to intramuscular fat content, lipid metabolism, meat color, tenderness and muscle growth. 23 853 and 34 731 novel transcripts located at the different sites of the pig genome were predicted in Min pig and Yorkshire pig, respectively, which including one base mismatch tags. Terms from the component ontology were 18, including cytoplasm, membrane-bounded organelle, pigment granule, lytic vacuole, extracellular region part, etc. Terms from the function ontology were 2, including protein binding and oxidoreductase activity. Terms from the process ontology were 14, including response to stress, response to reactive oxygen species and carboxylic acid metabolic process, etc. Terms from pathway were 11, according to the P value from small to large, lysosome, PPAR signaling pathway and primary bile acid biosynthesis were the most significantly different pathways. The results of this study could provide a theoretical basis for studying the novel genes or finding genetic factors affecting pork quality.