鸡白痢沙门菌感染太行鸡盲肠菌群的差异及功能分析

doi:10.11843/j.issn.0366-6964.2025.06.019

摘要/Abstract

摘要：

旨在分析鸡白痢沙门菌感染太行鸡后盲肠菌群的变化。采集19日龄未感染组(C组)和感染组(B组)太行鸡盲肠内容物，构建16S rRNA基因V3-V4区终文库，分析两组微生物菌群组成、丰度及多样性；利用Metastat和LEfSe分析方法寻找组间差异物种；利用PICRUSt进行基因功能预测；使用STAMP软件进行基因功能差异分析。结果发现C组潜在有益菌毛螺菌科(Lachnospiraceae)、丹毒丝菌科(Erysipelotrichaceae)和普拉梭菌(Faecalitalea_sp_)相对丰度显著高于B组(P < 0.05)。B组梭菌(Clostridiales_bacterium)相对丰度显著高于C组(P < 0.05)。PICRUSt基因功能预测表明，两组显著富集在6个主要代谢通路(P < 0.05)，其中B组微生物更多参与CMP-唾液酸合成酶中，可能影响鸡白痢沙门菌的感染。本研究通过鸡白痢沙门菌太行鸡感染模型探究盲肠微生物菌群特征，推测鸡白痢沙门菌可能通过梭菌(Clostridiales_bacterium)和CMP-唾液酸合成酶影响宿主肠道微生物菌群，为鸡白痢沙门菌感染的研究提供参考数据。

关键词: 太行鸡, 鸡白痢沙门菌, PICRUSt功能预测

Abstract:

The purpose of this study was to analyze the changes of cecal flora in Taihang chickens infected with Salmonella Pullorum. The cecal contents of 19-day-old Taihang chickens in uninfected group (group C) and infected group (group B) were collected. A final library of the 16S rRNA gene V3-V4 region was constructed, and the composition, abundance, and diversity of microbial flora in the two groups were analyzed. Metastat and LEfSe analysis were used to identify the different species between groups. PICRUSt was used to predict gene function. STAMP software was used to analyze gene function differences. The results showed that the relative abundance of potential beneficial bacteria in Lachnospiraceae, Erysipelotrichaceae and Faecalitalea_sp_ in group C were significantly higher than those in group B (P < 0.05). The relative abundance of Clostridiales_bacterium in group B was significantly higher than that in group C (P < 0.05). The prediction of PICRUSt gene function showed that the two groups were significantly enriched in six major metabolic pathways (P < 0.05), and the microorganisms in group B were more involved in CMP-sialic acid synthetase, which may affect the infection of Salmonella Pullorum. This study investigated the characteristics of cecal microflora flora using a Taihang chicken infection model of Salmonella Pullorum. It was hypothesized that Salmonella Pullorum may influence the host intestinal microbial flora through Clostridiales_bacterium and CMP-sialic acid synthase, providing reference data for research on Salmonella Pullorum infection.

Key words: Taihang chicken, Salmonella Pullorum, PICRUSt function prediction

中图分类号:

S831.2

董娇娇, 丁虹, 张寅梁, 张冉, 刘华格, 臧素敏, 张振红, 周荣艳, 李兰会. 鸡白痢沙门菌感染太行鸡盲肠菌群的差异及功能分析[J]. 畜牧兽医学报, 2025, 56(6): 2741-2751.

DONG Jiaojiao, DING Hong, ZHANG Yinliang, ZHANG Ran, LIU Huage, ZANG Sumin, ZHANG Zhenhong, ZHOU Rongyan, LI Lanhui. Differences and Functional Analysis of Cecal Flora in Taihang Chickens Infected with Salmonella Pullorum[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2741-2751.

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分类水平 Classification level	细菌 Bacteria	均值(C组) Mean(C group)	均值(B组) Mean(B group)	P值 P-value	Q值 Q value
科Family	毛螺菌科(Lachnospiraceae)	0.001 4	0	0.007	0.191
属Genus	霍尔德曼氏菌属(Holdemania)	0.067	0.	0.022	0.554
	ZOR0006	0.001 6	0.007	0.044	0.554
	其他(Other)	0.001 4	0	0.003	0.188
种 Species	梭菌 (Clostridiales_bacterium)	0.003 7	0.012	0.011	0.323
	g-霍尔德曼氏菌属s-未培养的细菌 (g_Holdemania s_uncultured_bacterium)	0.066 9	0	0.019	0.324
	g_ZOR0006 s_uncultured_bacterium	0.001 6	0.007	0.039	0.492
	f_spiraceae; Other Other	0.001 4	0	0.004	0.323
	g-果胶单核菌属其他 (g_Monoglobus Other)	0.000 2	0.001	0.040	0.492
	g-颤螺旋菌科；其他 (g_Oscillospiraceae; Other)	0.037 6	0.008	0.015	0.323
	s-未培养细菌 (s-uncultured_bacterium)	0.001 480 212	0.025 189 816	0.008	0.323

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