黄芩苷对猪繁殖与呼吸综合征病毒的体外抑制作用

doi:10.11843/j.issn.0366-6964.2025.05.025

摘要/Abstract

摘要：

旨在探讨黄芩苷对猪繁殖与呼吸综合征病毒(PRRSV)的体外抑制作用。利用实时定量聚合酶链式反应(RT-qPCR)、间接免疫荧光(IFA)、半数组织培养感染剂量(TCID₅₀)等试验观察黄芩苷对PRRSV感染阻断、直接杀灭、增殖抑制3种作用方式；吸附、内化、复制、释放4个复制环节以及与PRRSV病毒粒子直接相互作用的影响。结果发现，黄芩苷在不同作用方式下均可抑制PRRSV复制，感染阻断4 h时抑制作用显著(P < 0.05)；黄芩苷可以抑制PRRSV复制过程各个阶段，吸附和内化阶段抑制效果显著(P < 0.05)，且呈剂量依赖；黄芩苷可直接与PRRSV病毒粒子相互作用，抑制PRRSV复制(P < 0.05)。综上，黄芩苷在体外对PRRSV的复制具有抑制作用。

关键词: 黄芩苷, 猪繁殖与呼吸综合征病毒, 体外抑制作用

Abstract:

The aim of this study was to investigate the inhibitory effect of baicalin on porcine reproductive and respiratory syndrome virus (PRRSV) in vitro.Using real-time quantitative polymerase chain reaction (RT-qPCR), indirect immunofluorescence (IFA), half tissue culture infection dose (TCID₅₀) test to observe the three modes of action of baicalin on PRRSV, including infection blocking, direct killing and proliferation inhibition; and four steps including adsorption, internalization, replication and release; as well as the direct interaction with PRRSV virus particles. The results found that baicalin could inhibit PRRSV replication under different modes of action, and the inhibition effect was significantly at 4 h of infection blockade (P < 0.05); baicalin could inhibit the PRRSV replication process in the adsorption and internalization stages (P < 0.05) and was dose-dependently; baicalin could directly interact with PRRSV virions and inhibit PRRSV replication (P < 0.05). In conclusion, baicalin can inhibit PRRSV replication in vitro.

Key words: baicalin, porcine reproductive and respiratory syndrome virus, in vitro inhibition

中图分类号:

S852.659.6

吴非凡, 常伟辰, 孔亮, 张素梅, 黎露露, 徐曾浩, 张红英, 袁淑萍, 杨明凡. 黄芩苷对猪繁殖与呼吸综合征病毒的体外抑制作用[J]. 畜牧兽医学报, 2025, 56(5): 2270-2278.

WU Feifan, CHANG Weichen, KONG Liang, ZHANG Sumei, LI Lulu, XU Zenghao, ZHANG Hongying, YUAN Shuping, YANG Mingfan. Inhibitory Effect of Baicalin on Porcine Reproductive and Respiratory Syndrome Virus in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(5): 2270-2278.

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