参虎败毒颗粒体内抗猪繁殖与呼吸综合征病毒的效果观察

doi:10.11843/j.issn.0366-6964.2021.08.028

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (8): 2344-2353.doi: 10.11843/j.issn.0366-6964.2021.08.028

参虎败毒颗粒体内抗猪繁殖与呼吸综合征病毒的效果观察

李晨¹, 邱存义¹, 冯逸雪¹, 韩力康^2,3, 杜连昭¹, 王茜茜¹, 马欣¹, 李瑞香^2,3*, 王兴龙^1*

1. 西北农林科技大学动物医学院, 杨凌 712100;
2. 渭南市临渭区动物疫控中心, 渭南 714000;
3. 浙江金大康动物保健品有限公司, 金华 321016

收稿日期:2021-02-03 出版日期:2021-08-23 发布日期:2021-08-21
通讯作者: 李瑞香,主要从事动物疫病控制研究,E-mail:liruixianghappy@163.com;王兴龙,主要从事动物传染病防控研究,E-mail:wxlong@nwsuaf.edu.cn
作者简介:李晨(1994-),男,山西侯马人,硕士生,主要从事动物传染病防治研究,E-mail:Lchenxndy@163.com;邱存义(1996-),男,江西赣州人,硕士生,主要从事动物传染病防治研究,E-mail:15797637570@163.com;冯逸雪(1996-),女,新疆乌鲁木齐人,硕士生,主要从事动物传染病防治研究,E-mail:269022322@qq.com。
基金资助:
国家自然科学基金（31672581）；校企合作基金（K4030220282）

Observation of Anti-porcine Reproductive and Respiratory Syndrome Virus Effect of Shenhu Baidu Granules in vivo

LI Chen¹, QIU Cunyi¹, FENG Yixue¹, HAN Likang^2,3, DU Lianzhao¹, WANG Qianqian¹, MA Xin¹, LI Ruixiang^2,3*, WANG Xinglong^1*

1. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China;
2. Linwei District Animal Disease Prevention and Control Center, Weinan 714000, China;
3. Zhejiang Jindakang Animal Health Products Co. Ltd., Jinhua 321016, China

Received:2021-02-03 Online:2021-08-23 Published:2021-08-21

摘要/Abstract

摘要： 为探究参虎败毒颗粒抗猪繁殖与呼吸综合征病毒（PRRSV）的作用，将20头PRRSV阴性仔猪随机平均分为4组，即提前药物处理感染组（A组）、感染同时药物处理组（B组）、无药物处理感染组（C组）和无药物处理不感染组（D组）。A和B组分别于攻毒前3 d和攻毒时在基础日粮中添加参虎败毒颗粒（每头10 g·d^-1），连续饲喂至攻毒后28 d，每日测量体温，在攻毒后第14天，每组随机处死仔猪1头，观察病理变化，RT-qPCR检测肺组织病毒载量，并在攻毒后第1、3、5、7、14、21、28、35天采全血和血清样品进行PRRSV核酸和抗体检测。结果发现，A和B组仔猪体温在攻毒后第6天上升至40℃以上，第12天时恢复正常；C组仔猪体温在第5天上升至40℃以上，持续至试验结束仍未恢复。A、B组仔猪肺组织病变较轻，肺泡结构轻度损伤，支气管内有少量炎性渗出物，周围有少量炎性细胞浸润；C组仔猪肺组织病变严重，肺泡结构破坏严重，支气管内有大量炎性渗出物和炎性细胞浸润。A组在攻毒后3、5、7和14 d病毒载量极显著低于C组（P<0.01）；B组在攻毒后3和5 d病毒载量极显著低于C组（P<0.01），第7和14天病毒载量显著低于C组（P<0.05）。A、B组PRRSV抗体在攻毒后第21天达到峰值，C组在28 d达到峰值。以上结果可知，参虎败毒颗粒能够显著减轻PRRSV造成的仔猪肺部病理损伤，降低病毒血症；添加药物可使PRRSV特异性抗体峰值提前，减少病毒血症持续时间，证明参虎败毒颗粒可用于预防或治疗PRRSV感染，降低PRRSV对仔猪的影响。

关键词: 参虎败毒颗粒, 猪繁殖与呼吸综合征病毒, 病毒载量, 抗体

Abstract: In order to explore the effect of the Shenhu Baidu granules against porcine reproductive and respiratory syndrome virus (PRRSV), 20 piglets free of PRRSV were randomly divided into 4 groups. Group A were given medicine at 3 days before the infection and Group B were given medicine and infection at the same time. Group C and D were the infection and non-infection control, respectively. Medicine were mixed in the basal diet (10 g per pig per day) of A, B groups and supplied from the start time points to the end of the experiment. The rectal temperature of each piglet was recorded everyday. On the 14th day post infection (PI), one piglet in each group was randomly selected and euthanized to observe the lesions caused by viral infection. The viral loads in lungs were detected with RT-qPCR. And on day 1, 3, 5, 7, 14, 21, 28, and 35 PI, anticoagulant and non anticoagulant blood samples were collected for detection viral loads and antibodies, respectively. The rectal temperature of the piglets in Group A and B were raised up to 40℃ at 6th PI and returned to normal at the 11th and 12th days PI. While, high rectal temperature (more than 40℃) of the piglets in Group C lasted to the end of the experiment. The lung lesions in piglets from Group A and B were lighter and with less alveolar structure damage, inflammatory cell invasion and less exudate in the bronchus. However, the lesions in lung from Group C was serious. The alveolar structures were missing and the bronchus in the lung were filled with full of exudates. Serious inflammatory cell invasion were also observed around the bronchus. The viremia of the Group A were highly significantly lower than Group C at 3, 5, 7, and 14 days PI of PRRSV (P<0.01). And Group B was highly significantly lower than Group C at 3, 5 days PI (P<0.01), significantly lower at 7 and 14 days PI (P<0.05). Antibodies against PRRSV in Group A and B reached to the peak at 21 days PI, and the antibodies of the Group C reached to the peak at 28 days PI. In summary, the Shenhu Baidu granules significantly reduced the lesions caused by PRRSV and also the viral loads in bloods and lungs. The drug brought forward of the peak value of PRRSV-specific antibody and reduced the duration of viremia. This study proved that the Shenhu Baidu granules is effective in prevention and treating PRRSV infection.

Key words: Shenhu Baidu granules, porcine reproductive and respiratory syndrome virus, viral load, antibody

中图分类号:

S855.3

李晨, 邱存义, 冯逸雪, 韩力康, 杜连昭, 王茜茜, 马欣, 李瑞香, 王兴龙. 参虎败毒颗粒体内抗猪繁殖与呼吸综合征病毒的效果观察[J]. 畜牧兽医学报, 2021, 52(8): 2344-2353.

LI Chen, QIU Cunyi, FENG Yixue, HAN Likang, DU Lianzhao, WANG Qianqian, MA Xin, LI Ruixiang, WANG Xinglong. Observation of Anti-porcine Reproductive and Respiratory Syndrome Virus Effect of Shenhu Baidu Granules in vivo[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2344-2353.

参考文献 32

[1]	GOYAL S M.Porcine reproductive and respiratory syndrome[J].J Vet Diagn Invest,1993,5(4):656-664.
[2]	郭宝清,陈章水,刘文兴,等.从疑似PRRS流产胎儿分离PRRSV的研究[J].中国畜禽传染病,1996(2):1-5.GUO B Q,CHEN Z S,LIU W X,et al.Isolation and identification of porcine reproductory and respiratory syndrome (PRRS) virus from aborted fetuses suspected of PRRS[J].Chinese Journal of Animal and Poultry Infectious Diseases,1996(2):1-5.(in Chinese)
[3]	ZHOU Y J,HAO X F,TIAN Z J,et al.Highly virulent porcine reproductive and respiratory syndrome virus emerged in China[J].Transbound Emerg Dis,2008,55(3-4):152-164.
[4]	周磊,丁玉平.猪繁殖与呼吸综合征病毒类NADC30毒株的新近流行[J].中国猪业,2017,12(11):19-23,32.ZHOU L, DING Y P. The recent prevalence of NADC30 strain of porcine reproductive and respiratory syndrome virus[J].China Swine Industry,2017,12(11):19-23,32.(in Chinese)
[5]	WENSVOORT G,TERPSTRA C,POL J M A,et al.Mystery swine disease in The Netherlands:the isolation of Lelystad virus[J].Vet Quart,1991,13(3):121-130.
[6]	BENFIELD D A,NELSON E,COLLINS J E,et al.Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332)[J].J Vet Diagn Invest,1992,4(2):127-133.
[7]	KAPPES M A,MILLER C L,FAABERG K S.Porcine reproductive and respiratory syndrome virus nonstructural protein 2(NSP2) topology and selective isoform integration in artificial membranes[J].Virology,2015,481:51-62.
[8]	KAPPES M A,FAABERG K S.PRRSV structure,replication and recombination:Origin of phenotype and genotype diversity[J].Virology,2015,479-480:475-486.
[9]	JING H Y,FANG L R,DING Z,et al.Porcine reproductive and respiratory syndrome virus NSP1α inhibits NF-κB activation by targeting the linear ubiquitin Chain assembly complex[J].J Virol,2017,91(3):e01911-16.
[10]	MENG X J,PAUL P S,HALBUR P G,et al.Phylogenetic analyses of the putative M (ORF 6) and N (ORF 7) genes of porcine reproductive and respiratory syndrome virus (PRRSV):implication for the existence of two genotypes of PRRSV in the U.S.A. and Europe[J].Arch Virol,1995,140(4):745-755.
[11]	SUN L C,LI Y H,LIU R X,et al.Porcine reproductive and respiratory syndrome virus ORF5a protein is essential for virus viability[J].Virus Res,2013,171(1):178-185.
[12]	蔡丙严,田其真,刘莉,等.野大黄含药血清体外抑制猪繁殖与呼吸综合征病毒活性试验[J].中国动物传染病学报,2018,26(6):34-41.CAI B Y,TIAN Q Z,LIU L,et al.Inhibitory effect of wild Rhubarb serum on the activity of porcine reproductive and respiratory syndrome virus in vitro[J].Chinese Journal of Animal Infectious Diseases,2018,26(6):34-41.(in Chinese)
[13]	王飞.七清败毒散的药效学研究[D].杨凌:西北农林科技大学,2019.WANG F.Pharmacodynamics and safety test of Qiqingbaidusan abstract[D].Yangling:Northwest A & F University,2019.(in Chinese)
[14]	刘灿.高致病性猪繁殖与呼吸综合征病毒GD株不同代次毒株的体内特性研究[D].武汉:华中农业大学,2012.LIU C.Studies on characteristics of different generations of highly pathogenic porcine reproductive and respiratory syndrome virus (GD Strain) in vivo[D].Wuhan:Huazhong Agricultural University,2012.(in Chinese)
[15]	张春辉,邢雪琨,张慧.黄芩的药理作用研究[J].中兽医医药杂志,2020,39(2):98-100.ZHANG C H,XING X K,ZHANG H.Pharmacological effects of Radix Scutellariae[J].Journal of Traditional Chinese Veterinary Medicine,2020,39(2):98-100.(in Chinese)
[16]	张道广,KWANG J,潘胜利.黄芩多糖抗猪生殖和呼吸系统综合征病毒作用的研究[J].时珍国医国药,2005,16(9):943-944.ZHANG D G,KWANG J,PAN S L.Antiviral effect of polysaccharide from Scutellaria baicalensis Georgi on porcine respiratory and reproductive syndrome virus[J].Lishizhen Medicine and Materia Medica Research,2005,16(9):943-944.(in Chinese)
[17]	布玮玮.河南省PRRSV分子流行病学调查及体外抗病毒中药单体的筛选[D].洛阳:河南科技大学,2014.BU W W.Molecular epidemiological survey of PRRSV in Henan province and in vitro screening of antiviral traditional Chinese medicine unit[D].Luoyang:Henan University of Science and Technology,2014.(in Chinese)
[18]	解慧梅,蔡丙严,郝福星,等.栀子四黄散抗猪繁殖与呼吸综合征的研究[J].中国畜牧兽医,2020,47(11):3633-3640.XIE H M,CAI B Y,HAO F X,et al.Study on resistance of fructus Gardeniae Sihuang powder to porcine reproductive and respiratory syndrome[J].China Animal Husbandry & Veterinary Medicine,2020,47(11):3633-3640.(in Chinese)
[19]	李荣.虎杖苷对脂多糖诱导的小鼠子宫内膜炎的保护作用[D].长春:吉林大学,2020.LI R.Protective effects of polydatin on LPS-induced endometritis in mice[D].Changchun:Jilin University,2020.(in Chinese)
[20]	李萍,程晓馨.白藜芦醇抗菌抗病毒作用的研究进展[J].中国微生态学杂志,2014,26(10):1215-1219.LI P,CHENG X X.The effects of resveratrol on antibacterial and antiviral properties[J].Chinese Journal of Microecology,2014,26(10):1215-1219.(in Chinese)
[21]	顾文松,沈明华,刘伟,等.中药复方提取物对细胞抗猪繁殖与呼吸综合征病毒感染能力的影响[J].养猪,2009(4):73-74.GU W S,SHEN M H,LIU W,et al.Anti-infectious potential of Mare-145 cell treated by Chinese herbal extract in PRRSV challenge test[J].Swine Production,2009(4):73-74.(in Chinese)
[22]	李长玲,蔺娇,栾园园,等.白头翁药理作用研究进展[J].畜牧与饲料科学,2019,40(3):88-91.LI C L,LIN J,LUAN Y Y,et al.Research progress of pharmacological effects of Pulsatilla chinensis[J].Animal Husbandry and Feed Science,2019,40(3):88-91.(in Chinese)
[23]	胡梅.几种不同中药成分体外抗病毒作用的活性研究[D].郑州:河南农业大学,2007.HU M.Reactive study on anti-virus of several Chinese herbal medicinal ingredients in vitro[D].Zhengzhou:Henan Agricultural University,2007.(in Chinese)
[24]	胡安君.苦参碱、甘草酸体外抗猪繁殖与呼吸综合征病毒机制研究[D].郑州:河南农业大学,2016.HU A J.The mechanism of matrine and glycyrrhizic acid against porcine reproductive and respiratory syndrome virus[D]. Zhengzhou:Henan Agricultural University, 2016.(in Chinese)
[25]	孙盼盼.苦参碱对感染PRRSV Marc-145细胞ERK1/2信号通路和细胞受体表达的影响[D].晋中:山西农业大学,2016.SUN P P. Effect of Matrine on ERK1/2 signaling pathway and the receptor expression of Marc-145 cells infected with PRRSV[D]. Jinzhong:Shanxi Agricultural University, 2016.(in Chinese)
[26]	王学斌.黄芪、板蓝根等中药提取物及组方体内免疫调节和体外抗病毒作用研究[D].郑州:河南农业大学,2007.WANG X B.Immunomodulatory activity in vivo and antiviral effect in vitro of traditional Chinese herbal medicine extracts and compounds:Radix Astragali,Radix Isatidis etc[D].Zhengzhou:Henan Agricultural University,2007.(in Chinese)
[27]	刘樱,丁度伟,高求炜,等.3种中药及其提取物体外抗猪繁殖与呼吸综合征病毒作用的研究[J].中国畜牧兽医,2016,43(10):2730-2735.LIU Y,DING D W,GAO Q W,et al.Antiviral effects of three Chinese herbal medicine and their polysaccharides on porcine reproductive and respiratory syndrome virus (PRRSV) in vitro[J].China Animal Husbandry & Veterinary Medicine,2016,43(10):2730-2735.(in Chinese)
[28]	袁威,李展,张平,等.中药体外抗PRRSV研究进展[J].中国兽医杂志,2018,54(4):52-56.YUAN W, LI Z, ZHANG P, et al. Research progress of anti-PRRSV effect of traditional Chinese medicine in vitro[J]. Chinese Journal of Veterinary Medicine, 2018, 54(4):52-56. (in Chinese)
[29]	左小娟.板蓝根提取物治疗猪蓝耳病病毒血症临床效果的研究[D].成都:四川农业大学,2017.ZUO X J.The clinical effect of Chinese medicine "Radix extract" on controlling viremia of PRRS[D].Chengdu:Sichuan Agricultural University,2017.(in Chinese)
[30]	王惠.板蓝根配合不同中药制剂对PRRSV抗体及核酸载量的影响[D].成都:四川农业大学,2019.WANG H.Effects of Radix isatidis combined with different preparations of traditional Chinese medicine on PRRSV antibody and nucleic acid load[D].Chengdu:Sichuan Agricultural University,2019.(in Chinese)
[31]	林昌华,蒋家霞,钟孙雄,等.丁酸月桂酸甘油二酯对后备母猪PRRSV驯化效果的影响[J].饲料研究,2020,43(6):34-36.LIN C H,JIANG J X,ZHONG S X,et al.Effect of survilipos on PRRSV domestication of gilts[J].Feed Research,2020,43(6):34-36.
[32]	郭锡钦.α-单月桂酸甘油酯对仔猪免疫功能及猪繁殖与呼吸综合征病毒的影响研究[D].临安:浙江农林大学,2019.GUO X Q.Effects of α-glyceryl monolaurate on immune function and reproductive and respiratory syndrome virus in piglets[D].Lin'an:Zhejiang A&F University,2019.(in Chinese)

参虎败毒颗粒体内抗猪繁殖与呼吸综合征病毒的效果观察

Observation of Anti-porcine Reproductive and Respiratory Syndrome Virus Effect of Shenhu Baidu Granules in vivo

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