多能性干细胞概述及其在家畜上的研究进展

doi:10.11843/j.issn.0366-6964.2025.04.001

摘要/Abstract

摘要：

多能性干细胞具有无限增殖潜力和分化成多种类型组织细胞的能力，是当前干细胞研究的热点和焦点，家畜多能性干细胞的研究不仅具有基础理论意义，在家畜繁殖育种、生物医学、食品生产等领域具有重要的应用价值。本文以胚胎干细胞和诱导性多能干细胞为例，对多能性干细胞的多能性状态及培养条件、鉴定方法进行概述，综述了家畜多能性干细胞的研究进展，并对其应用前景进行了展望，以期为家畜多能性干细胞的研究提供参考。

关键词: 多能性, 干细胞, 胚胎干细胞, 诱导性多能干细胞

Abstract:

Pluripotent stem cells (PSCs) have unlimited proliferation potential and the ability to differentiate into various types of tissue cells, making them the focus of current stem cell research. The research on PSCs in livestock not only holds fundamental theoretical significance, but also has important implications for application in animal breeding, biomedicine, food production and other fields. This review provides a comprehensive summary of the culture conditions, identification methods, and maintenance of pluripotency in pluripotent stem cells. Furthermore, it outlines the research progress of livestock pluripotent stem cells and provides a prospective outlook on its application prospects in order to offer references in further study of livestock pluripotent stem cells.

Key words: pluripotency, stem cells, embryonic stem cells, induced pluripotent stem cells

中图分类号:

S852.2

王佳美, 黄永震, 高晨, 李俊良, 陈燕, 朱波, 张路培, 王泽昭, 高会江, 李俊雅, 高雪. 多能性干细胞概述及其在家畜上的研究进展[J]. 畜牧兽医学报, 2025, 56(4): 1473-1483.

WANG Jiamei, HUANG Yongzhen, GAO Chen, LI Junliang, CHEN Yan, ZHU Bo, ZHANG Lupei, WANG Zezhao, GAO Huijiang, LI Junya, GAO Xue. Research Progress and Overview on Pluripotent Stem Cells in Livestock[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1473-1483.

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培养基Medium	配方Formula	细胞类型Cell type
FBS/LIF^[25]	基础培养基：KO-DMEM；FBS 15%、GlutaMAX 1%、NEAA 1%、双抗1%、β-巯基乙醇0.1 mmol· L^-1、hLIF 10 ng·mL^-1	胚胎干细胞、诱导性多能干细胞
2i/LIF^[29]	基础培养基N2B27：DMEM/F-12 50%、Neurobasal 50%、N2 0.5%、B27 1%、双抗1%、β-巯基乙醇0.1 mmol· L^-1；hLIF：10 ng·mL^-1、CHIR99021：1 μmol· L^-1、PD0325901：3 μmol· L^-1	胚胎干细胞、诱导性多能干细胞
3i/LIF^[30]	2i/LIF+A8301	胚胎干细胞、诱导性多能干细胞
3i/LAF^[23]	基础培养基：N2B27、KnockOut Serum Replacement 5%、NEAA 1%、VC 50μg·mL^-1；CHIR99021 1 umol· L^-1、Activin A 25 ng·mL^-1、WH-4-023 1 μmol· L^-1、hLIF 10 ng·mL^-1、FGF2 10 ng·mL^-1、IWR-1-endo 2.5 μmol· L^-1、Y-27632(2 μmol· L^-1维持，10 μmol· L^-1传代)	原肠化前上胚层多能性干细胞
LCDM^[24]	基础培养基：N2B27；10 ng·mL^-1 hLIF、CHIR 99021(人：1 μmol· L^-1，小鼠：3 μmol· L^-1)(S)-(+)-马来酸二甲苯茚酸酯2 μmol· L^-1、盐酸米诺环素2 μmol· L^-1	山羊iPSCs、扩展多能干细胞、牛滋养层干细胞

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