牦牛AIF-1蛋白表达及对巨噬细胞炎性因子mRNA的影响

doi:10.11843/j.issn.0366-6964.2020.05.028

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (5): 1163-1166.doi: 10.11843/j.issn.0366-6964.2020.05.028

• 研究简报 • 上一篇

牦牛AIF-1蛋白表达及对巨噬细胞炎性因子mRNA的影响

李娟¹, 王利^1*, 罗晓林^2*, 官久强², 安添午², 张翔飞²

1. 西南民族大学青藏高原动物遗传资源保护与利用教育部和四川省重点实验室, 成都 610041;
2. 四川省草原科学研究院, 成都 611731

收稿日期:2019-10-25 出版日期:2020-05-25 发布日期:2020-05-16
通讯作者: 王利,主要从事动物免疫学研究,E-mail:qinxin916@aliyun.com;罗晓林,主要从事动物遗传育种与繁殖研究,E-mail:Luoxl2004@sina.com
作者简介:李娟(1995-),女,四川西昌人,硕士生,主要从事动物遗传学研究,E-mail:1134323458@qq.com
基金资助:
国家十三五重点研发计划项目（2018YFD0502304）；国家肉牛牦牛产业技术体系（CARS-37）；四川省科技支撑计划（16ZC2530）；第二次青藏高原综合科学考察研究项目（2019QZKK0302）；四川省留学人员科技活动择优资助项目（2019）；西南民族大学中央高校基本科研业务费专项资金项目（2020NYB30）

Expression of Yak AIF-1 Protein and Its Effect on mRNA of Inflammatory Factors in Macrophagocyte

LI Juan¹, WANG Li^1*, LUO Xiaolin^2*, GUAN Jiuqiang², AN Tianwu², ZHANG Xiangfei²

1. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province Southwest Minzu University, Chengdu 610041, China;
2. Sichuan Academy of Grassland Sciences, Chengdu 611731, China

Received:2019-10-25 Online:2020-05-25 Published:2020-05-16

摘要/Abstract

摘要： 旨在对牦牛同种移植炎症因子-1（allograft inflammatory factor-1，AIF-1）蛋白进行原核表达、纯化，并探讨其对巨噬细胞炎性因子的影响。采用q-PCR检测AIF-1基因在牦牛5种组织中的表达量，构建原核表达载体表达纯化AIF-1蛋白，q-PCR检测小鼠巨噬细胞4种炎性因子的表达量。结果表明，AIF-1基因在麦洼牦牛脾中表达水平最高，极显著高于其它组织（P<0.01）。表达并纯化出约29.47 ku的AIF-1重组蛋白，1.0、10.0、100.0 μg·mL^-1 AIF-1蛋白均能促进小鼠巨噬细胞IL-1β、IL-6、TNF-α和iNOS的表达。这表明AIF-1在巨噬细胞免疫应答中发挥着一定作用，为深入研究牦牛AIF-1功能提供参考。

关键词: 同种移植炎症因子-1, 原核表达, 巨噬细胞, 炎性因子

Abstract: The objective of this study was to express the allograft inflammatory factor-1 (AIF-1) of yak in E.coli BL21(DE3) and investigate its effect on mouse macrophages. The expression levels of AIF-1 gene in 5 tissues of yak were detected by q-PCR. A prokaryotic expression vector was constructed to express and purify AIF-1 protein. The expression levels of 4 inflammatory factors in mouse macrophages were detected by q-PCR. The results showed that the expression level of AIF-1 gene was the highest in the spleen of Maiwa yak, which was extremely significantly higher than those in other tissues (P<0.01). Recombinant protein of approximately 29.47 ku was expressed and purified. The expression levels of IL-1β, IL-6, TNF-α and iNOS in mouse macrophages were promoted by 1.0, 10.0 and 100.0 μg·mL^-1 of AIF-1 protein. The results indicated that AIF-1 played a role in the immune response of macrophages. This article will be benefit for further study on the function of yak AIF-1.

Key words: allograft inflammatory factor-1, prokaryotic expression, macrophage, inflammatory factor

中图分类号:

S823.85

李娟, 王利, 罗晓林, 官久强, 安添午, 张翔飞. 牦牛AIF-1蛋白表达及对巨噬细胞炎性因子mRNA的影响[J]. 畜牧兽医学报, 2020, 51(5): 1163-1166.

LI Juan, WANG Li, LUO Xiaolin, GUAN Jiuqiang, AN Tianwu, ZHANG Xiangfei. Expression of Yak AIF-1 Protein and Its Effect on mRNA of Inflammatory Factors in Macrophagocyte[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1163-1166.

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牦牛AIF-1蛋白表达及对巨噬细胞炎性因子mRNA的影响

Expression of Yak AIF-1 Protein and Its Effect on mRNA of Inflammatory Factors in Macrophagocyte

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