酿酒葡萄皮渣对单栏饲养方式下公绵羊繁殖性能及睾丸抗氧化性的影响

doi:10.11843/j.issn.0366-6964.2016.11.010

摘要/Abstract

摘要：

旨在研究日粮中添加一定量的酿酒葡萄皮渣对绵羊繁殖性能及睾丸抗氧化性能的影响。试验选取30只5月龄，体重(25±1)kg的杜泊×小尾寒羊杂交公羊，采用完全随机设计平均分为5组。1组自由活动，其余4组单栏饲养，建立应激模型。自由活动绵羊饲喂未添加葡萄皮渣日粮，单栏饲养绵羊分别饲喂不同葡萄皮渣添加水平（0%、5%、10%、20%）日粮。试验期80 d。试验结束后，屠宰取样，测定睾丸系数并作附睾精子分析，测定睾丸组织抗氧化水平，对睾丸组织中Cu-ZnSOD、CAT、GPx4及Nrf2 mRNA和蛋白相对表达量进行研究。结果表明，与自由活动组相比，单栏组绵羊睾丸组织中MDA及ROS水平升高（P<0.01），睾丸重量显著降低（P<0.05），睾丸系数也表现出下降趋势（P=0.06），附睾精子密度及顶体完整率显著降低（P<0.05），精子活动率极显著降低（P<0.01），精子畸形率极显著升高（P<0.01），表明单栏饲养方式对绵羊造成氧化应激，并在一定程度上影响绵羊繁殖性能。适量添加酿酒葡萄皮渣后，绵羊附睾精子密度和精子活动率均显著升高（P<0.05），精子畸形率显著降低（P<0.05），睾丸组织MDA含量显著下降（P<0.01），SOD、CAT及GSH-Px活性均显著提高（P<0.01，P<0.05，P<0.01）；睾丸组织中Cu-ZnSOD mRNA相对表达量显著提高（P<0.05），Cu-ZnSOD、CAT、GPx4蛋白相对表达量显著提高（P<0.05）。综上表明，酿酒葡萄皮渣可以提高单栏饲养方式下绵羊睾丸组织抗氧化性，进而改善绵羊繁殖性能。

Abstract:

This experiment was conducted to investigate the effect of wine grape pomace supplementation to base diet on reproductive performance and antioxidant ability of testis in ram. A total of thirty 5-month-old with average weight of (25±1) kg Dorper (♂) × Small Tail Han sheep (♀) F1 male lambs were randomly selected and divided into 5 groups equally. To establish a stress model, lambs in one of groups were raised freely, while the other 4 groups were confined in stall. Lambs raised freely were fed with a base diet without grape pomace, and other lambs were fed with diets supplemented with grape pomace at 0%, 5%, 10%, 20%, respectively. The experiment lasted for 80 d. At the end of experiment, all lambs were slaughtered and sampled. Testis coefficient, epididymal sperm indicators, antioxidant levels of testis were analyzed, and moreover, expression of Cu/ZnSOD, CAT, GPx4, Nrf2 mRNA and protein in testis were detected. The results showed that, compared with free group, MDA and ROS level of testis significantly increased (P<0.01), testis weight of lambs significantly decreased (P<0.05), and the organ coefficient of testis had a trend to be lower (P=0.06) in confined group with 0% wine grape pomace; epididymal sperm density, acrosomal integrity and motility were lower (P<0.05 and P<0.01, respectively), while epididymal sperm deformity higher (P<0.01), in confined group with 0% wine grape pomace than those in free group. These results showed that ram raised in confined condition could cause oxidative stress and affect the reproductive performance. However, compared with confined group with 0% wine grape pomace, epididymal sperm density and motility were higher, while deformity lower, in wine grape pomace-added groups (P<0.05). MDA level of testis significantly decreased (P<0.01), but SOD, CAT and GSH-Px activity significantly increased (P<0.01,P<0.05 and P<0.01) in wine grape pomace-added groups. Furthermore, the mRNA expression of Cu-ZnSOD and protein expression of Cu-ZnSOD, GPx4 and CAT in testis significantly increased (P<0.05) in wine grape pomace-added groups. The results indicate that wine grape pomace can enhance antioxidant capacity in testis and improve the reproductive performance of separated-raising lambs.

中图分类号:

S826.2

金亚倩，刘文忠,赵俊星，任有蛇，张春香，张文佳，项斌伟，马雪豪，张建新. 酿酒葡萄皮渣对单栏饲养方式下公绵羊繁殖性能及睾丸抗氧化性的影响[J]. 畜牧兽医学报, 2016, 47(11): 2228-2239.

JIN Ya-qian, LIU Wen-zhong, ZHAO Jun-xing, REN You-she, ZHANG Chun-xiang, ZHANG Wen-jia, XIANG Bin-wei, MA Xue-hao, ZHANG Jian-xin. Effect of Dietary Wine Grape Pomace on Reproductive Performance, Antioxidant Ability of Testis in Ram[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2228-2239.

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参考文献

［1］赵天章, 张慧鲜, 王文义, 等. 集约化饲养模式下巴美肉羊与小尾寒羊产肉性能的比较研究［J］. 中国农业大学学报, 2014, 19(4):121-128.
ZHAO T Z, ZHANG H X, WANG W Y, et al. Comparison meat-productivities between Bamei sheep and Small-tail Han sheep under intensive feeding pattern［J］. Journal of China Agricultural University, 2014, 19(4):121-128. (in Chinese)
［2］MONTALDO H H, FLORES-SERRANO C, SULAIMAN Y, et al. Growth and reproductive performance of Poll Dorset and Suffolk sheep under intensive conditions［J］.Rev Mex de Cienc Pecuarias, 2011, 2(4):359-369.
［3］卢珍珍, 郑琛, 李发弟, 等. 葡萄渣对羔羊生产性能、屠宰性能和养分消化代谢的影响［J］.草业学报, 2015, 24(4):114-120.
LU Z Z, ZHENG C, LI F D, et al. Effect of feeding grape pomace on nutrient digestibility, body weight gain, and carcass quality in lambs［J］. Acta Prataculturae Sinica, 2015, 24(4):114-120. (in Chinese)
［4］O′BRIEN N M, ROSEMARY C, O′CALLAGHAN Y C, et al. Modulatory effects of resveratrol, citroflavan-3-ol, and plant-derived extracts on oxidative stress in U937 cells［J］. J Med Food, 2006, 9(2):187-195.
［5］CAILLET S, SALMIÉRI S, LACROIX M. Evaluation of free radical-scavenging properties of commercial grape phenol extracts by a fast colorimetric method［J］. Food Chem, 2006, 95(1):1-8.
［6］SHI J, YU J,POHORLY J E, et al. Polyphenolics in grape seeds-biochemistry and functionality［J］. J Med Food, 2003, 6(4):291-299.
［7］GESSNER D K, FIESEL A, MOST E, et al. Supplementation of a grape seed and grape marc meal extract decreases activities of the oxidative stress-responsive transcription factors NF-κB and Nrf2 in the duodenal mucosa of pigs［J］. Acta Vet Scand, 2013, 55:18.
［8］CHOY Y Y,QUIFER-RADA P, HOLSTEGE D M, et al. Phenolic metabolites and substantial microbiome changes in pig feces by ingesting grape seed proanthocyanidins［J］. Food Funct, 2014, 5(9):2298-2308.
［9］CHAMORRO S, VIVEROS A, CENTENO C, et al. Effects of dietary grape seed extract on growth performance, amino acid digestibility and plasma lipids and mineral content in broiler chicks［J］. Animal, 2013, 7(4):555-561.
［10］AITKEN R J, SMITH T B, JOBLING M S, et al. Oxidative stress and male reproductive health［J］. Asian J Androl, 2004, 16(1):31-38.
［11］AITKEN R J, JONES K T, ROBERTSON S A. Reactive oxygen species and sperm function-in sickness and in health［J］. J Androl, 2012, 33(6):1096-1106.
［12］BITGUL G, TEKMEN I, KELES D, et al. Protective effects of resveratrol against chronic immobilization stress on testis［J］. ISRN Urol, 2013,2013(6):278720.
［13］SU L, DENG Y, ZHANG Y, et al. Protective effects of grape seed procyanidin extract against nickel sulfate-induced apoptosis and oxidative stress in rat testes［J］. Toxicol Mech Methods, 2011, 21(6):487-494.
［14］龚灵芝. 基于自由基的动物繁殖机能的损伤与修复机理及其调控技术研究［D］. 上海：上海交通大学, 2009.
GONG L Z. Study on injury and repair mechanisms of animal reproductive function and regulative technology based on free radical［D］.Shanghai：Shanghai Jiao Tong University, 2009. (in Chinese)
［15］汤建平, 蔡辉益, 常文环, 等. 饲养密度与饲粮能量水平对肉仔鸡生长性能及肉品质的影响［J］. 动物营养学报, 2012, 24(2):239-251.
TANG J P, CAI H Y, CHANG W H, et al. Effects of stocking density and dietary energy level on growth performance and meat quality of broilers［J］. Chinese Journal of Animal Nutrition, 2012, 24(2):239-251.(in Chinese)
［16］吕琼霞, 张书霞, 赵茹茜. 运输应激对猪免疫机能的影响及其细胞因子的调控［J］. 中国农业科学, 2009, 42(7):2579-2585.
LÜ Q X, ZHANG S X, ZHAO R Q. Effects of transportation stress on immune function and regulation of cytokines in pigs［J］. Scientia Agricultura Sinica,2009, 42(7):2579-2585. (in Chinese)
［17］张林, 岳洪源, 张海军,等. 不同强度的运输应激对肉仔鸡血液应激指标和肌肉品质的影响［J］. 动物营养学报, 2009, 21(3):288-293.
ZHANG L, YUE H Y, ZHANG H J, et al. Effects of different intensities of transport on blood stress parameters and meat quality in broiler chicks［J］. Chinese Journal of Animal Nutrition, 2009, 21(3):288-293. (in Chinese)
［18］SAHIN E, GÜMÜSLÜ S. Immobilization stress in rat tissues: Alterations in protein oxidation, lipid peroxidation and antioxidant defense system［J］. Comp Biochem Physiol C Toxicol Pharmacol, 2007, 144(4):342-347.
［19］ALMEIDA S A, PETENUSCI S O, ANSELMOFRANCI J A, et al. Decreased spermatogenic and androgenic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty［J］. Braz J Med Biol Res,1998, 31(11):1443-1448.
［20］RAI J，PANDEY S N,SRIVASTAVA R K. Testosterone hormone level in albino rats following restraint stress of long duration［J］. J Anat Soc India,2004, 53(1):17-19.
［21］PRIYA P H, REDDY P S. Effect of restraint stress on lead-induced male reproductive toxicity in rats［J］. J Exp Zool A Ecol Genet Physiol, 2012, 317(7):455-465.
［22］PRIYA P H, GIRISH B P, REDDY P S. Restraint stress exacerbates alcohol-induced reproductive toxicity in male rats［J］. Alcohol, 2014, 48(8):781-786.
［23］MESSAOUDI I, BANNI M, SAÏD L, et al. Involvement of selenoprotein P and GPx4 gene expression in cadmium-induced testicular pathophysiology in rat［J］. Chem Biol Interact, 2010, 188(1):94-101.
［24］SARADHA B, MATHUR P P. Induction of oxidative stress by lindane in epididymis of adult male rats［J］. Environ Toxicol Pharmacol, 2006, 22(1):90-96.
［25］CHANDRA A K, GHOSH R, CHATTERJEE A, et al. Effects of vanadate on male rat reproductive tract histology, oxidative stress markers and androgenic enzyme activities［J］. J Inorg Biochem, 2007, 101(6):944-956.
［26］张晋青, 岳度兵, 罗海玲, 等. 日粮中维生素E水平对敖汉细毛羊内脏器官生长发育的影响［J］. 中国畜牧杂志, 2010, 46(17):43-46.
ZHANG J Q, YUE D B, LUO H L, et al. Effects of dietary vitamin E on growth and development of internal organs in Aohan Fine Wool sheep［J］. Chinese Journal of Animal Science, 2010, 46(17):43-46. (in Chinese)
［27］POVLSEN B B, AW L D, LAURSEN R J, et al. Pregnancy and birth after intracytoplasmic sperm injection with normal testicular spermatozoa in a patient with azoospermia and tail stump epididymal sperm［J］. Int Braz J Urol, 2015, 41(6):1220-1225.
［28］UGURALP S, USTA U, MIZRAK B. Resveratrol may reduce apoptosis of rat testicular germ cells after experimental testicular torsion［J］. Eur J Pediatr Surg, 2005, 15(5):333-336.
［29］XU M, WEI Q, ZHENG K, et al. Protective effects of Big-leaf mulberry and physiological roles of nitric oxide synthases in the testis of mice following water immersion and restraint stress［J］. Acta Histochem, 2014, 116(8):1323-1330.
［30］YASSA H A, GEORGE S M, REFAIY AEL R, et al. Camellia sinensis (green tea) extract attenuate acrylamide induced testicular damage in albino rats［J］. Environ Toxicol, 2014, 29(10):1155-1161.
［31］SIMONETTI P, CIAPPELLANO S, GARDANA C, et al. Procyanidins from Vitis vinifera seeds: in vivo effects on oxidative stress［J］. J Agric Food Chem, 2002, 50(21):6217-6221.
［32］NATELLA F, BELELLI F, GENTILI V, et al. Grape seed proanthocyanidins prevent plasma postprandial oxidative stress in humans［J］. J Agric Food Chem, 2002, 50(26):7720-7725.
［33］秦小伟. 日粮中添加纳米锌对绵羊精液品质和睾丸、附睾中Cu-ZnSOD表达的影响［D］. 太谷：山西农业大学, 2014.
QIN X W. Effect of Nano-zinc diets on sheep semen quality and gonads Cu-ZnSOD expression［D］. Taigu:Shanxi Agricultural University, 2014. (in Chinese)
［34］王宇光. 基于药物代谢酶的中药相互作用研究［D］.北京：中国人民解放军军事医学科学院, 2006.
WANG Y G. Study of interaction of Chinese herbal medicine based on drug metabolism enzymes［D］. Beijing:Academy of Military Medical Sciences, 2006. (in Chinese)
［35］MCMAHON M, ITOH K, YAMAMOTO M, et al. Keap1-dependent proteasomal degradation of transcription factor Nrf2 contributes to the negative regulation of antioxidant response element-driven gene expression［J］. J Biol Chem,2003, 278(24):21592-21600.
［36］ZHANG D D. The Nrf2-Keap1-ARE signaling pathway: The regulation and dual function of Nrf2 in cancer［J］. Antioxid Redox Signal, 2010, 13(11):1623-1626.
［37］BAK M J, JUN M, JEONG W S. Procyanidins from wild grape (Vitis amurensis) seeds regulate ARE-mediated enzyme expression via Nrf2 coupled with p38 and PI3K/Akt pathway in HepG2 cells［J］. Int J Mol Sci, 2012, 13(1):801-818.
［38］LI C, XU X, TAO Z, et al. Resveratrol dimers, nutritional components in grape wine, are selective ROS scavengers and weak Nrf2 activators［J］. Food Chem, 2015, 173:218-223.
［39］陈志辉,任皓威,徐良梅.女贞子对AA肉鸡肌肉抗氧化能力及GPx4基因表达的影响［J］.中国畜牧杂志, 2013, 49(5):53-56.
CHEN Z H, REN H W, XU L M. Effect of Ligustrum Lucidum on muscle antioxidant capacity and Gpx4 gene expression of AA broilers［J］. Chinese Journal of Animal Science, 2013, 49(5):53-56. (in Chinese)
［40］KOZIOROWSKA-GILUN M, GILUN P, FRASER L, et al. Antioxidant enzyme activity and mRNA expression in reproductive tract of adult male European Bison ( Bison bonasus, Linnaeus 1758)［J］. Reprod Domest Anim, 2013, 48(1):7-14.
［41］王永章, 张大鹏. 苹果果实发育过程中α-淀粉酶的活性、数量变化和亚细胞定位［J］.植物学报:英文版, 2002, 44(1):34-41.
WANG Y Z, ZHANG D P. Activities, quantitative changes and subcellular localization of α-amylase during development of apple fruit［J］. Acta Botanica Sinica, 2002, 44(1):34-41.(in Chinese)

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