卵泡期和黄体期山羊垂体miRNAs表达谱及差异分析

doi:10.11843/j.issn.0366-6964.2016.11.009

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (11): 2218-2227.doi: 10.11843/j.issn.0366-6964.2016.11.009

卵泡期和黄体期山羊垂体miRNAs表达谱及差异分析

凌英会^1,2，朱龙^1,2，睢梦华^1,2，郑琪^1,2，吴昊^1,2，张运海^1,2，章孝荣^1,2,3*

（1.安徽农业大学动物科技学院，合肥 230036； 2.安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036；3.安徽省羊繁育工程技术研究中心，合肥 230036）

收稿日期:2016-06-30 出版日期:2016-11-23 发布日期:2016-11-23
通讯作者: 章孝荣，教授，博士生导师，主要从事动物生殖调控研究， E-mail：zhangxiaorong01@163.com
作者简介:凌英会(1981-)，男，安徽安庆人，副教授，博士，主要从事动物遗传育种与繁殖研究，E-mail：caaslyh@163.com
基金资助:
国家自然科学基金项目（31301934;31372310）;安徽省现代农业产业技术体系（2016-2020）

Comparative Profiling of Differentially Expressed microRNAs between the Follicular and Luteal Phases Pituitary of Goats

LING Ying-hui^1,2,ZHU Long^1,2,SUI Meng-hua^1,2,ZHENG Qi^1,2,WU Hao^1,2,ZHANG Yun-hai^1,2,ZHANG Xiao-rong^1,2,3*

(1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,China; 2. Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei 230036,China；3. Engineering Research Center of Reproduction and Breeding in Sheep of Anhui Province, Hefei 230036,China)

Received:2016-06-30 Online:2016-11-23 Published:2016-11-23

摘要/Abstract

摘要：

旨在通过高通量测序的方法筛选与分析卵泡期和黄体期安淮山羊垂体中差异表达miRNAs，探索垂体在转录后水平调节山羊从卵泡期-黄体期过渡中发挥的作用。测序结果表明，卵泡期和黄体期文库分别得到11 319 069和11 432 846条原始序列。经过去除杂质后，得到11 162 888和11 016 138条纯净序列。在两个不同时期的文库中，分别检测到148和150种差异表达的miRNA，有147种miRNA共同表达；分别预测到52和95种差异表达的miRNA，有27种miRNA两文库共同表达。已知miRNA中，let-7f在两文库表达量最高，且差异极显著；新miRNA中，novel-miR-159在两文库表达量最高，且差异极显著。通路分析表明，ko00511、ko00052、ko01100、ko00030、ko00520 5个通路可能影响性激素的合成。测序获得安淮山羊垂体组织miRNA序列及表达谱，垂体组织miRNA表达丰富，且表达量各异。研究结果有助于理解miRNA在山羊垂体中调节性激素分泌的作用，并且所鉴别的miRNA有助于后续卵泡期～黄体期转变的相关研究。

Abstract:

In this study, we used Solexa sequencing to screen and analyze the differentially expressed microRNAs (miRNAs) of Anhuai goat pituitary tissues in the follicular phase (Fols) and luteal phase (Luts), and explore the role of pituitary at post-transcriptional level of Fols to Luts transition occurred in goats. In total, 11 319 069 and 11 432 846 raw reads were obtained from the ovaries of Anhuai goats in Fols and Luts, respectively, after eliminating impurity, 11 162 888 and 11 016 138 clean reads. 147 known miRNAs were co-expressed in the two different periods phases, 148 and 150 known miRNAs were expressed in the ovary in the Fols and Luts, respectively. In addition, 27 novel miRNAs were co-expressed in the two phases, 52 and 95 novel miRNAs were expressed in the ovary in the Fols and Luts, respectively. Let-7f was the highest expressed significantly different known miRNAs in the two phases, and miR-159 was the highest expressed significantly different novel miRNAs in the two phases, which may participate in the follicular-luteal transition of Anhuai goats. In the KEGG pathway analysis, ko00511, ko00052, ko01100, ko00030 and ko00520 may be related to the synthesis of sex hormones. The study succeeded to construct the expression library of miRNAs which were abundant and differentially expressed in Anhuai goat pituitary tissues. The results will help to further understand the role of miRNAs which regulates secretion of hormone participate in goat pituitary. And some identified miRNAs will help to further understand the role of miRNAs in the regulation of follicular to luteal transition in goat ovaries.

中图分类号:

S827.2

凌英会，朱龙，睢梦华，郑琪，吴昊，张运海，章孝荣. 卵泡期和黄体期山羊垂体miRNAs表达谱及差异分析[J]. 畜牧兽医学报, 2016, 47(11): 2218-2227.

LING Ying-hui,ZHU Long,SUI Meng-hua,ZHENG Qi,WU Hao,ZHANG Yun-hai,ZHANG Xiao-rong. Comparative Profiling of Differentially Expressed microRNAs between the Follicular and Luteal Phases Pituitary of Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2218-2227.

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卵泡期和黄体期山羊垂体miRNAs表达谱及差异分析

Comparative Profiling of Differentially Expressed microRNAs between the Follicular and Luteal Phases Pituitary of Goats

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