不同旱养方式对鹅睾丸及外生殖器组织形态学的影响

doi:10.11843/j.issn.0366-6964.2023.07.019

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (7): 2872-2885.doi: 10.11843/j.issn.0366-6964.2023.07.019

不同旱养方式对鹅睾丸及外生殖器组织形态学的影响

卿恩华^1,2, 唐彬铖^1,2, 牛添¹, 王珺琦^1,2, 陈朝颜¹, 胡继伟¹, 何桦¹, 李亮^1,2, 王继文^1,2*, 胡深强^1,2*

1. 四川农业大学 "畜禽遗传资源发掘与创新利用"四川省重点实验室, 成都 611130;
2. 四川农业大学动物遗传育种研究所, 农业农村部畜禽生物组学重点实验室, 成都 611130

收稿日期:2022-09-15 出版日期:2023-07-23 发布日期:2023-07-21
通讯作者: 王继文,主要从事水禽遗传育种与养殖技术方面的研究,E-mail:wjw2886166@163.com;胡深强,主要从事水禽遗传育种与养殖技术方面的研究,E-mail:shenqiang.hu@sicau.edu.cn
作者简介:卿恩华(1998-),男,四川都江堰人,硕士,主要从事水禽遗传育种与养殖技术方面的研究,E-mail:qingenhua@yeah.net
基金资助:
四川省科技计划项目（2021YFYZ0014）；四川省自然科学基金面上项目（2023NSFSC0227）；国家现代水禽产业技术体系项目（CARS-42-4）

Effects of Different Dry Rearing Systems on Histomorphology of Goose Testis and External Genitalia

QING Enhua^1,2, TANG Bincheng^1,2, NIU Tian¹, WANG Junqi^1,2, CHEN Zhaoyan¹, HU Jiwei¹, HE Hua¹, LI Liang^1,2, WANG Jiwen^1,2*, HU Shenqiang^1,2*

1. Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;
2. Key Laboratory of Livestock and Poultry Multi-omics of Ministry of Agriculture and Rural Affairs, Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China

Received:2022-09-15 Online:2023-07-23 Published:2023-07-21

摘要/Abstract

摘要： 旨在探究不同旱养方式对公鹅生殖系统发育的影响。以同批孵化、健康的100只四川白鹅公鹅为试验对象，于120日龄时从中挑选60只体重相近的公鹅等分为2组（每组30只），分别进行笼养和网上地面混合平养，继续饲养至270日龄时屠宰并采集鹅睾丸及外生殖器进行组织形态学分析。形态学结果显示：笼养组鹅左侧、右侧和双侧睾丸重量及其器官指数极显著高于混合平养组（P<0.01），笼养组鹅右侧睾丸长径以及两侧睾丸短径显著高于混合平养组（P<0.05）；笼养组鹅外生殖器的自然长度和基部直径显著高于混合平养组（P<0.05），拉直长度极显著高于混合平养组（P<0.01）。组织学结果显示：笼养组鹅睾丸中精原细胞数量显著高于混合平养组（P<0.05），睾丸实质间质比、生精上皮厚度、支持细胞数量和生殖细胞数量极显著高于混合平养组（P<0.01）；笼养组鹅的外生殖器角化上皮厚度显著低于混合平养组（P<0.05）。相关性分析结果显示：鹅睾丸或外生殖器的形态学与组织学指标之间存在显著相关性（P<0.05），且两种饲养方式下鹅睾丸与外生殖器的组织形态学指标之间均存在显著相关性（P<0.05），但笼养组鹅睾丸与外生殖器组织形态学指标之间的相关性更强。综上，两种旱养方式下鹅睾丸与外生殖器的发育过程均密切相关；相较于混合平养，120日龄上笼且持续150 d的笼养方式显著促进了公鹅生殖系统发育及睾丸生精能力。

关键词: 公鹅, 笼养, 混合平养, 睾丸, 外生殖器, 组织形态学

Abstract: The purpose of this study was to explore the effects of different dry rearing systems on the reproductive system development of male geese. Taking 100 healthy male Sichuan White geese hatched from the same batch as the experimental subjects, at 120 days of age 60 male geese with similar body weights were selected and equally divided into two groups (30 individuals per group), which were reared under the cage-rearing system (CRS) and net-floor mixed rearing system (MRS), respectively, and were slaughtered at 270 days of age to collect the testes and external genitalia for subsequent histomorphological analysis. Morphological results showed that the weight and organ index of geese left-, right- and bilateral testes were extremely significantly higher in CRS than that in MRS (P<0.01), and the long diameter of geese right testis and short diameter of geese bilateral testis was significantly higher in CRS than that in MRS (P<0.05). The natural length and basal diameter of geese external genitalia were significantly higher in CRS than that in MRS (P<0.05), and the straightened length was extremely significantly higher in CRS than that in MRS (P<0.01). Histological results showed that the number of geese testicular spermatogonia was significantly higher in CRS than that in MRS (P<0.05), and the ratio of testicular parenchyma to interstitium, the thickness of seminiferous epithelium, the number of sertoli cells and the number of germ cells were extremely significantly higher in CRS than that in MRS (P<0.01). The thickness of keratinized epithelium of geese external genitalia was significantly lower in CRS than that in MRS (P<0.05). The results of correlation analysis showed that there were significant correlations between the morphological and histological parameters of either geese testes or external genitalia (P<0.05); moreover, there were significant correlations in the histomorphological indicators between geese testes and external genitalia under both rearing systems (P<0.05), but the correlations in the histomorphological indicators between geese testes and external genitalia in CRS were much stronger. Taken together, the developmental processes of geese testes and external genitalia are closely related under both dry rearing systems. Compared with MRS, the 150-day period of CRS starting from 120 days of age significantly promotes the reproductive system development of male geese and the testicular spermatogenic ability.

Key words: male goose, cage rearing system, net-floor mixed rearing system, testis, external genitalia, histomorphology

中图分类号:

S835.3

卿恩华, 唐彬铖, 牛添, 王珺琦, 陈朝颜, 胡继伟, 何桦, 李亮, 王继文, 胡深强. 不同旱养方式对鹅睾丸及外生殖器组织形态学的影响[J]. 畜牧兽医学报, 2023, 54(7): 2872-2885.

QING Enhua, TANG Bincheng, NIU Tian, WANG Junqi, CHEN Zhaoyan, HU Jiwei, HE Hua, LI Liang, WANG Jiwen, HU Shenqiang. Effects of Different Dry Rearing Systems on Histomorphology of Goose Testis and External Genitalia[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2872-2885.

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不同旱养方式对鹅睾丸及外生殖器组织形态学的影响

Effects of Different Dry Rearing Systems on Histomorphology of Goose Testis and External Genitalia

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