性成熟期辽宁绒山羊与子午岭黑山羊睾丸发育比较

doi:10.11843/j.issn.0366-6964.2021.012.013

摘要/Abstract

摘要： 本研究旨在探究性成熟期辽宁绒山羊与子午岭黑山羊睾丸发育是否存在差异，并对两品种繁殖性能进行比较。选取性成熟期健康的辽宁绒山羊和子午岭黑山羊各5只，采集睾丸组织，通过大体解剖和苏木精-伊红（HE）染色石蜡切片，比较两品种山羊睾丸组织发育及形态学差异；ELISA检测雄激素浓度；实时荧光定量PCR （real time quantitative PCR，RT-qPCR）、蛋白免疫印迹（Western blot）检测两品种山羊睾丸组织中死盒多肽4（DEAD box polypeptide 4，DDX4）和类无精症缺失基因（deleted in azoospermia-like gene，DAZL）的表达情况。结果显示，辽宁绒山羊睾丸总重和睾丸长周径极显著高于子午岭黑山羊（P<0.01），而睾丸短周径、睾丸脏体比和睾丸胴体比均差异不显著（P>0.05）；辽宁绒山羊生精上皮厚度显著高于子午岭黑山羊（P<0.05），而两者精细管面积、直径和单位面积内精细管数量均无显著差异（P>0.05）；两品种山羊睾丸中雄激素分泌无显著差异（P>0.05）；辽宁绒山羊DDX4 mRNA及蛋白表达量均显著高于子午岭黑山羊（P<0.01或P<0.05），DAZL mRNA表达量极显著高于子午岭黑山羊（P<0.01），而蛋白表达量差异不显著（P>0.05）。以上结果表明，性成熟期辽宁绒山羊性腺发育程度与子午岭黑山羊一致，但生精上皮较子午岭黑山羊厚，生殖标记基因表达量存在差异，推测可能会影响两品种的生精能力。

关键词: 辽宁绒山羊, 子午岭黑山羊, 睾丸, DDX4, DAZL

Abstract: The study aimed to investigate the differences in testicular development between Liao-ning cashmere goat and Ziwuling black goat at sexual maturity and to compare the reproductive performance between the two breeds. In this study, testicular tissues were collected from five healthy Liaoning cashmere goats and five healthy Ziwuling black goats at sexual maturity stage. The testicular tissue development and morphological differences between the two goat breeds were compared using anatomy and Hematoxylin-eosin (HE) stained, respectively. The concentration of androgen were detected using ELISA assay. The expression of DEAD box polypeptide 4 (DDX4) and Deleted in azoospermia-like gene (DAZL) in testis of the two breeds were detected using Real-time quantitative PCR(RT-qPCR) and Western blot technique. The study revealed that the total weight and length circumference of testicular in Liaoning cashmere goats were significantly higher than those in Ziwuling black goats (P<0.01), while the difference testicular breadth circumference, total testicular weight/pre-slaughter live weight and total testicular weight/carcass weight between the two goat breeds were not significant (P>0.05). The spermatogenic epithelial thickness of Liaoning cashmere goats was significantly higher than that of Ziwuling black goats (P<0.05), there were no significant differences in the area and diameter of the seminal tubules, number of seminal tubules per unit area between the two goat breeds (P>0.05). The difference of androgen secretion in testis between the two goat breeds was not significant (P>0.05). The expression levels of DDX4 mRNA and DDX4 protein in the testis of Liaoning cashmere goats were significantly higher than those of Ziwuling black goats (P<0.01 or P<0.05). The expression levels of DAZL mRNA in the testis of Liaoning cashmere goats was significantly higher than that of Ziwuling black goats (P<0.01), while the expression of DAZL protein between two goat breeds was not significant (P>0.05). In short, the degree of gonad development in Liaoning cashmere goats was identical with Ziwuling black goats, while the spermatogenic epithelium was thicker than Ziwuling black goats, there were relevant difference in the expression levels of reproductive marker genes between the two goat breeds, which might affect the spermatogenic ability between the two breeds.

Key words: Liaoning cashmere goat, Ziwuling black goat, testes, DDX4, DAZL

中图分类号:

S827.3

梁维炜, 李明娜, 罗玉柱, 王继卿, 柯娜, 沈继源, 郝志云, 金夏阳, 鲁玉洁, 黄兆春. 性成熟期辽宁绒山羊与子午岭黑山羊睾丸发育比较[J]. 畜牧兽医学报, 2021, 52(12): 3461-3470.

LIANG Weiwei, LI Mingna, LUO Yuzhu, WANG Jiqing, KE Na, SHEN Jiyuan, HAO Zhiyun, JIN Xiayang, LU Yujie, HUANG Zhaochun. Comparation of the Testes Development between Liaoning Cashmere Goat and Ziwuling Black Goat at Sexual Maturity[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3461-3470.

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