CRISPR/Cas9系统在寄生原虫基因编辑中的应用

doi:10.11843/j.issn.0366-6964.2023.01.007

摘要/Abstract

摘要： 原虫生活史复杂，大部分难以通过体外培养完成整个生活史，缺乏高效的基因编辑系统。CRISPR/Cas9系统可以通过人工设计的方式，实现基因的精确、高效、快速编辑，被广泛应用于基因工程各个领域。CRISPR/Cas9系统为原虫的未知功能基因研究开辟了新途径。本文介绍CRISPR/Cas9系统的原理和目前在寄生原虫中的基因定位、基因功能研究、高通量基因家族筛选等方面的应用。

关键词: CRISPR/Cas9, 基因编辑, 寄生虫, 原虫

Abstract: Protozoa have complex life cycle and most of them can not complete the life cycle just in vitro. As a result, most of them lack an effective gene editing system. The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (endonuclease) system facilitates the precise, efficient, and rapid genetic editing through homologous recombination, and is widely used in genetic modification of eukaryotic organisms. The CRISPR/Cas9 system opens up a new avenue for targeted investigation of gene functions in protozoans. This article introduces the principle of the CRISPR/Cas9 system and its major applications in gene tagging and depletion and genome-wide screening of functional gene families in parasitic protozoan.

Key words: CRISPR/Cas9, genetic editing, parasite, protozoa

中图分类号:

S852.73

邓敏儿, 李娜, 郭亚琼, 冯耀宇, 肖立华. CRISPR/Cas9系统在寄生原虫基因编辑中的应用[J]. 畜牧兽医学报, 2023, 54(1): 69-79.

DENG Min'er, LI Na, GUO Yaqiong, FENG Yaoyu, XIAO Lihua. Application of CRISPR/Cas9 System on Gene Editing of Parasitic Protozoa[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 69-79.

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