1株奶牛源约翰逊不动杆菌的分离鉴定

doi:10.11843/j.issn.0366-6964.2022.01.032

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (1): 324-328.doi: 10.11843/j.issn.0366-6964.2022.01.032

• 研究简报 • 上一篇

1株奶牛源约翰逊不动杆菌的分离鉴定

田秋丰, 张红, 尹珺伊*, 史同瑞, 张军, 刘秋瑾, 王岩, 秦平伟, 王欢, 白长胜, 陈楠楠, 朱庆贺, 苗艳

黑龙江省农业科学院畜牧兽医分院, 齐齐哈尔 161005

收稿日期:2021-04-22 出版日期:2022-01-23 发布日期:2022-01-26
通讯作者: 尹珺伊,主要从事兽医微生物学研究,E-mail:346250622@qq.com
作者简介:田秋丰(1988-),男,满族,吉林四平人,研究实习员,硕士,主要从事兽医微生物学研究,E-mail:qiufeng1221@163.com
基金资助:
黑龙江省农业科学院“农业科技创新跨越工程”奶牛提质增效技术研究与示范项目（HNK2019CX15）

Isolation and Identification of a Strain Acinetobacter johnsonii from Dairy Cow

TIAN Qiufeng, ZHANG Hong, YIN Junyi*, SHI Tongrui, ZHANG Jun, LIU Qiujin, WANG Yan, QIN Pingwei, WANG Huan, BAI Changsheng, CHEN Nannan, ZHU Qinghe, MIAO Yan

Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihaer 161005, China

Received:2021-04-22 Online:2022-01-23 Published:2022-01-26

摘要/Abstract

摘要： 旨在了解从奶牛垫料中分离得到1株革兰阴性菌的病原特征，本研究通过形态学观察、生化试验、染色镜检、16S rDNA同源性分析等方法对分离菌进行鉴定，并采用药敏试验测定药物敏感性，小鼠攻毒试验确定分离菌株毒力情况。结果表明，分离菌为革兰阴性菌；扩增的16S rDNA序列长度为1 283 bp，与约翰逊不动杆菌的核苷酸相似性大于99%；分离菌株对小鼠有较强的致病性，试验组小鼠死亡率达80%。分离菌对链霉素、复方新诺明等6种药物敏感，对头孢噻肟、头孢呋辛中度敏感，对青霉素、红霉素等4种药物耐药。本研究从奶牛垫料中分离到1株约翰逊不动杆菌，为奶牛源约翰逊不动杆菌的防治和研究提供了参考依据。

关键词: 约翰逊不动杆菌, 奶牛, 16S rDNA, 药敏分析

Abstract: In order to understand the pathogenic characteristics of a Gram-negative strain isolated from cows bedding litters.In this study, the isolates bacterium were identified by morphological observation, biochemical test, staining microscopy, 16S rDNA homology analysis. Drug sensitivity test was used to determine drug sensitivity, mice attack test was used to determine the virulence of the isolates. The results showed that the isolates were Gram-negative bacteria. The nucleotide sequence of 16S rDNA was 1 283 bp, the nucleotide homology with Acinetobacter johnsonii is greater than 99%. The isolated bacterium had strong pathagenicity to mice, the mortality was 80% in test group. The isolates were sensitive to 6 drugs including streptomycin, compound sulfamethoxazole, moderately sensitive to cefotaxime and cefuroxime, resistant to 4 drugs including penicillin and erythromycin. In this study, a strain of Acinetobacter johnsonii was isolated from dairy cow bedding litters, which provides a reference for the prevention and treatment of Acinetobacter johnsonii.

Key words: Acinetobacter johnsonii, diary cows, 16S rDNA, drug sensitivity test

中图分类号:

S852.61

田秋丰, 张红, 尹珺伊, 史同瑞, 张军, 刘秋瑾, 王岩, 秦平伟, 王欢, 白长胜, 陈楠楠, 朱庆贺, 苗艳. 1株奶牛源约翰逊不动杆菌的分离鉴定[J]. 畜牧兽医学报, 2022, 53(1): 324-328.

TIAN Qiufeng, ZHANG Hong, YIN Junyi, SHI Tongrui, ZHANG Jun, LIU Qiujin, WANG Yan, QIN Pingwei, WANG Huan, BAI Changsheng, CHEN Nannan, ZHU Qinghe, MIAO Yan. Isolation and Identification of a Strain Acinetobacter johnsonii from Dairy Cow[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 324-328.

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1株奶牛源约翰逊不动杆菌的分离鉴定

Isolation and Identification of a Strain Acinetobacter johnsonii from Dairy Cow

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