畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (11): 3175-3184.doi: 10.11843/j.issn.0366-6964.2021.011.019
李洋, 高胜, 焦点, 杜兴乾, 徐乐乐, 郑紫方, 曹鑫宇, 肖书奇*
收稿日期:
2021-03-01
出版日期:
2021-11-23
发布日期:
2021-11-24
通讯作者:
肖书奇,主要从事动物病原感染致病与免疫机制、新型疫苗与诊断技术研发,E-mail:xiaoshuqi@nwsuaf.edu.cn
作者简介:
李洋(1993-),男,内蒙古呼和浩特人,博士,主要从事猪PRRS疫苗研发,E-mail:15109252976@163.com;高胜(1994-),男,山东济宁人,硕士,主要从事猪病免疫防控研究,E-mail:1040186891@qq.com。
基金资助:
LI Yang, GAO Sheng, JIAO Dian, DU Xingqian, XU Lele, ZHENG Zifang, CAO Xinyu, XIAO Shuqi*
Received:
2021-03-01
Online:
2021-11-23
Published:
2021-11-24
摘要: 本研究拟评估仔猪接种猪繁殖与呼吸综合征减毒活疫苗对猪圆环病毒病疫苗、猪瘟疫苗免疫的干扰情况,并分析不同免疫方式对仔猪生长性能的影响,以期为探究PRRSV减毒活疫苗与PCV2、CSF疫苗的联合免疫提供数据参考。本研究先以100头仔猪为研究对象,将其随机分为A、B、C、D 4组。其中A组仔猪免疫PRRSV减毒活疫苗7 d后免疫PCV2疫苗;B组仔猪同期分点注射PRRSV减毒活疫苗和PCV2疫苗;C组仔猪仅免疫PCV2疫苗;D组仔猪仅免疫PRRSV减毒活疫苗。另外再筛选100头仔猪,随机分为E、F、G、H 4组。E组仔猪于免疫PRRSV减毒活疫苗12 d后免疫CSF疫苗;F组仔猪同期分点注射PRRSV减毒活疫苗和CSF疫苗;G组仔猪仅免疫CSF疫苗;H组仔猪仅免疫PRRSV减毒活疫苗。免疫4周后,测定仔猪血清中相关抗体水平。同时,称量免疫前后各组仔猪的体重,计算不同免疫方案仔猪的平均日增重。结果表明:A~D组中, A组和B组仔猪在免疫4周后均产生了较高水平的PRRSV及PCV2抗体,且A组抗体水平略高于B组。C组仔猪仅产生了PCV2抗体,D组仔猪产生了较高水平的PRRSV抗体。E~H 4组中,E组和F组仔猪均产生了较高水平的PRRSV及CSFV抗体。G组仔猪仅产生了高水平的CSFV抗体,H组仔猪仅产生了高水平的PRRSV抗体。A、B、C、D 4组中B组仔猪的平均日增重最高;而E、F、G、H 4组中E组仔猪的平均日增重显著高于其他3组。PRRSV减毒活疫苗与PCV2疫苗或CSF疫苗同期分点免疫、免疫PRRSV减毒活疫苗一段时间后再免疫PCV2或CSF疫苗均能诱导仔猪产生高水平的抗体;在体液免疫方面,PRRSV减毒活疫苗免疫与否均未对另外二种疫苗表现出明显的干扰作用。在仔猪28日龄时同期分点免疫PRRSV减毒活疫苗与PCV2疫苗,12 d后再免疫CSF疫苗的免疫方案不仅能诱导仔猪产生高水平抗体,还可以使仔猪具备较高的平均日增重。
中图分类号:
李洋, 高胜, 焦点, 杜兴乾, 徐乐乐, 郑紫方, 曹鑫宇, 肖书奇. PRRSV-VR2332减毒活疫苗不影响猪瘟疫苗和猪圆环病毒2型灭活疫苗诱导的抗体产生[J]. 畜牧兽医学报, 2021, 52(11): 3175-3184.
LI Yang, GAO Sheng, JIAO Dian, DU Xingqian, XU Lele, ZHENG Zifang, CAO Xinyu, XIAO Shuqi. PRRSV-VR2332 Modified Live Attenuated Vaccine does not Affect the Antibody Production Induced by Classical Swine Fever Vaccine and Inactivated Porcine Circovirus Type 2 Vaccine[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3175-3184.
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