基于网络药理学探讨葛根芩连汤治疗猪腹泻的靶点与通路

doi:10.11843/j.issn.0366-6964.2020.11.026

摘要/Abstract

摘要： 本研究旨在探讨葛根芩连汤治疗猪腹泻的靶点与通路。利用中药系统药理学分析平台（TCMSP）检测葛根芩连汤的化学成分和靶点，运用cytoscape 3.7.1软件构建并分析化合物-靶点网络、蛋白相互作用（PPI）网络和核心靶点通路网络。结果显示，经筛选后，共获得大豆苷元（daidzein）、葛根素（puerarin）、汉黄芩苷（oroxindin）、芒柄花素（formononetin）等60个活性成分，这些成分作用206个靶点，其中，TP53、TNF、IL-6、IL-8、JUN、STAT3等为核心靶点，这些核心靶点涉及磷脂酰肌醇3-激酶／蛋白激酶B信号通路、病毒致癌机制等。基于网络药理学分析结果，葛根芩连汤可能是通过TP53、TNF、IL-6、IL-8、JUN、STAT3等靶点调控腹泻与发热，本文为葛根芩连汤在猪腹泻治疗中的深入研究提供理论依据与线索。

关键词: 葛根芩连汤, 猪, 腹泻, 网络药理学

Abstract: This experiment was conducted to explore the mechanism of Gegen Qinlian decoction in treating swine diarrhea. The chemical composition and targets of Gegen Qinlian decoction were searched by the analysis of the traditional Chinese medicine system pharmacology platform (TCMSP). The software of Cytoscape 3.7.1 was used to construct and analyze compound-targets networks, protein-protein interaction (PPI) network and key targets-signaling pathway network. The results showed that 60 active compounds including daidzein, puerarin, oroxindin and formononetin were obtained after screening. The compounds acted on 206 targets, among which TP53, TNF, IL-6, IL-8, Jun and STAT3 were the key targets. These key targets involve in PI3K-Akt signaling pathway, Viral carcinogenesis, and so on. Based on the results of network pharmacological analysis, Gegen Qinlian decoction may regulate diarrhea and fever through TP53, TNF, IL-6, IL-8, Jun, STAT3 and other targets. The paper provides theoretical basis and clues for the further study of Gegen Qinlian decoction in the treatment of swine diarrhea.

Key words: Gegen Qinlian decoction, swine, diarrhea, network pharmacology

中图分类号:

S853.74

王春花, 孙雪芳. 基于网络药理学探讨葛根芩连汤治疗猪腹泻的靶点与通路[J]. 畜牧兽医学报, 2020, 51(11): 2875-2885.

WANG Chunhua, SUN Xuefang. The Target and Pathway of Gegen Qinlian Decoction in Treating Swine Diarrhea Based on Network Pharmacology[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2875-2885.

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