粪菌移植法治疗非特异病原性羔羊腹泻的效果初报

doi:10.11843/j.issn.0366-6964.2020.08.011

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (8): 1878-1885.doi: 10.11843/j.issn.0366-6964.2020.08.011

粪菌移植法治疗非特异病原性羔羊腹泻的效果初报

王燕¹, 滕晓晓¹, 杨柠芝¹, 李婷¹, 陈卓¹, 斯坎达尔·伊斯拉木¹, 王治¹, 祁来芳¹, 刘佳佳², 杨会国², 赵红琼^1*, 姚刚^1*

1. 新疆农业大学动物医学学院, 乌鲁木齐 830052;
2. 新疆畜牧科学院畜牧研究所, 乌鲁木齐, 830026

收稿日期:2020-03-02 出版日期:2020-08-25 发布日期:2020-08-19
通讯作者: 姚刚,主要从事动物生长发育与畜产品质量安全研究,E-mail:yg@xjau.edu.cn;赵红琼,主要从事生物活性物质结构和功能研究,E-mail:zhaohongqiong@sina.com
作者简介:王燕(1994-),女,回族,江苏丰县人,硕士生,主要从事反刍幼畜生长发育与调控的研究,E-mail:1311156742@qq.com;滕晓晓(1991-),女,山东肥城人,硕士生,主要从事动物疾病防治的研究,E-mail:184674717@qq.com
基金资助:
新疆自治区重点研发计划项目（82017B01005-2-4）

Preliminary Report of the Therapeutic Effect of Fecal Microbiota Transplantation on Non-specific Pathogenic Diarrhea in Suckling Lambs

WANG Yan¹, TENG Xiaoxiao¹, YANG Ningzhi¹, LI Ting¹, CHEN Zhuo¹, Eskandar·eslam¹, WANG Zhi¹, QI Laifang¹, LIU Jiajia², YANG Huiguo², ZHAO Hongqiong^1*, YAO Gang^1*

1. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China;
2. Institute of Animal Science, Xinjiang Academy of Animal Sciences, Urumqi 830026, China

Received:2020-03-02 Online:2020-08-25 Published:2020-08-19

摘要/Abstract

摘要： 羔羊腹泻是一种常发生于新生和断奶阶段，且能够导致羔羊生长迟滞甚至死亡的消化道疾病。粪菌移植（fecal microbiota transplantation，FMT）技术在反刍幼畜消化道疾病防治上鲜有研究。本研究采用FMT对腹泻羔羊进行治疗并与不同抗生素药物治疗进行对比，探索FMT对吮乳期羔羊腹泻的治疗效果。试验选择日龄（24±2）d、体重（6.12±1.01）kg、临床散发的具有典型腹泻症状的吮乳期羔羊60只（公母各半）以及健康羔羊10只（公母各半），分为健康对照组（HC）、腹泻对照组（SC）、粪菌移植治疗组（FMT）、庆大霉素治疗组（GM）、恩诺沙星治疗组（ENR）、庆大霉素+粪菌移植治疗组（GM+FMT）以及恩诺沙星+粪菌移植治疗组（ENR+FMT），测定腹泻羔羊腹泻治愈天数、日增重以及羔羊生理生化指标及相关炎性因子指标。结果表明：1）腹泻羔羊平均日增重、红细胞数量、血红蛋白含量、淋巴细胞比例和肌酐浓度均显著低于健康对照组羔羊（P<0.05），而平均血红蛋白浓度和炎性因子均显著高于健康对照组（P<0.05）；2）与SC组相比，经过5种方法治疗，除GM+FMT组外，FMT、ENR、ENR+FMT和GM组均显著降低治愈天数（P<0.05）。ENR组还显著提高腹泻羔羊日增重（P<0.05）；3）与SC组相比，FMT治疗可显著提升腹泻羔羊血液中血红蛋白含量和血液淋巴细胞数量（P<0.05）；4）经FMT、ENR和GM+FMT治疗后，各种炎性因子均显著下降到健康组羔羊水平。结果表明，FMT能够有效治愈羔羊腹泻症状，血液生理生化功能得到改善，抑制了炎性反应，显著降低腹泻率。FMT有望作为一种抗生素替代方法应用于羔羊腹泻防治实践。

关键词: 羔羊, 腹泻, 粪菌移植, 生理生化指标, 炎性因子

Abstract: Lamb diarrhea is one of the common gastrointestinal diseases during the newborn and weaning stage that can result in lamb's growth retardation and even death. Fecal microbiota transplantation (FMT) has little been applied on the prevention and treatment of digestive tract diseases in young ruminants. In this study, FMT was used to treat diarrhea lambs with the comparison by using different antibiotics so as to explore the therapeutic effect of FMT on suckling lambs' diarrhea. Sixty suckling lambs (male and female in half) with significant clinical symptoms of diarrhea and 10 healthy lambs (male and female in half) at the age of (24±2) days and body weight of (6.12±1.01) kg were divided into 7 groups, namely, healthy control (HC), saline control (SC), fecal microbiota transplantation (FMT), gentamicin (GM), enrofloxacin (ENR), gentamicin+FMT (GM+FMT) and enrofloxacin+FMT (ENR+FMT) treatment, with 10 lambs each. The healing time (day), average daily gain (ADG), physio-biochemical indexes and the diarrhea related inflammatory factor markers were measured. The results showed: 1) ADG, RBC, HGB, the percentage of lymphocyte and the concentration of serum creatinine (CREA) in SC group were all significantly lower than those of HC group (P<0.05), whereas the MCHC and inflammatory factors in SC group were significantly higher than those of HC group (P<0.05). 2) Compared with SC group, after 5 methods of treatment, the FMT, ENR, ENR + FMT, and GM groups all significantly reduced the healing time (P<0.05) except for the GM + FMT group. Only after ENR treatment the ADG was significantly increased (P<0.05). 3) In comparison with SC group, FMT treatment significantly increased the blood hemoglobin content (HGB) and blood lymphocyte number (P<0.05). 4) After FMT, ENR and GM+FMT treatment the inflammatory factors measured were all significantly declined to the level as that in healthy lambs (P<0.05). It is indicated that FMT could treat the lamb's diarrhea effectively by shortening the healing time, improvement of physio-biochemical statues and the inhibition of inflammation in diarrheic lambs. FMT could be an alternative way of lambs' diarrhea treatment by antibiotics in practice.

Key words: lamb, diarrhea, fecal microbiota transplantation, blood physio-biochemical index, inflammatory factor

中图分类号:

S826
S857.4

王燕, 滕晓晓, 杨柠芝, 李婷, 陈卓, 斯坎达尔·伊斯拉木, 王治, 祁来芳, 刘佳佳, 杨会国, 赵红琼, 姚刚. 粪菌移植法治疗非特异病原性羔羊腹泻的效果初报[J]. 畜牧兽医学报, 2020, 51(8): 1878-1885.

WANG Yan, TENG Xiaoxiao, YANG Ningzhi, LI Ting, CHEN Zhuo, Eskandar·eslam, WANG Zhi, QI Laifang, LIU Jiajia, YANG Huiguo, ZHAO Hongqiong, YAO Gang. Preliminary Report of the Therapeutic Effect of Fecal Microbiota Transplantation on Non-specific Pathogenic Diarrhea in Suckling Lambs[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 1878-1885.

参考文献 26

[1]	LYNCH S V,PEDERSEN O.The human intestinal microbiome in health and disease[J].N Engl J Med,2016,375(24):2369-2379.
[2]	MALMUTHUGE N,GRIEBEL P J,GUAN L L.The gut microbiome and its potential role in the development and function of newborn calf gastrointestinal tract[J].Front Vet Sci,2015,2:36.
[3]	WANG S Q,MA T,ZHAO G H,et al.Effect of age and weaning on growth performance,rumen fermentation,and serum parameters in lambs fed starter with limited ewe-lamb interaction[J].Animals,2019,9(10):825.
[4]	POP M,WALKER A W,PAULSON J,et al.Diarrhea in young children from low-income countries leads to large-scale alterations in intestinal microbiota composition[J].Genome Biol,2014,15:R76.
[5]	ROUND J L,PALM N W.Causal effects of the microbiota on immune-mediated diseases[J].Sci Immunol,2018,3(20):eaao1603.
[6]	CHAITMAN J,JERENS A E,GASCHEN F,et al.Commentary on key aspects of fecal microbiota transplantation in small animal practice[J].Vet Med (Auckl),2016,7:71-74.
[7]	KELLY C R,KAHN S,KASHYAP P,et al.Update on fecal microbiota transplantation 2015:indications,methodologies,mechanisms,and outlook[J].Gastroenterology,2015,149(1):223-237.
[8]	MINTZ M,KHAIR S,GREWAL S,et al.Longitudinal microbiome analysis of single donor fecal microbiota transplantation in patients with recurrent Clostridium difficile infection and/or ulcerative colitis[J].PLoS One,2018,13(1):e0190997.
[9]	NIEDERWERDER M C.Fecal microbiota transplantation as a tool to treat and reduce susceptibility to disease in animals[J].Vet Immunol Immunopathol,2018,206:65-72.
[10]	DUPONT H L,JIANG Z D,DUPONT A W,et al.Abnormal intestinal microbiome in medical disorders and potential reversibility by fecal microbiota transplantation[J].Dig Dis Sci,2020,65(3):741-756.
[11]	HENSLEY-MCBAIN T,ZEVIN A S,MANUZAK J,et al.Effects of fecal microbial transplantation on microbiome and immunity in simian immunodeficiency virus-infected macaques[J].J Virol,2016,90(10):4981-4989.
[12]	FURMANSKI S,MOR T.First case report of fecal microbiota transplantation in a cat in israel[J].J Vet Med,2017,72(3):35-41.
[13]	MULLEN K R,YASUDA K,DIVERS T J,et al.Equine faecal microbiota transplant:Current knowledge,proposed guidelines and future directions[J].Equine Vet Educ,2018,30(3):151-160.
[14]	PEREIRA G Q,GOMES L A,SANTOS I S,et al.Fecal microbiota transplantation in puppies with canine parvovirus infection[J].J Vet Intern Med,2018,32(2):707-711.
[15]	SMITS L P,BOUTER K E C,DE VOS W M,et al.Therapeutic potential of fecal microbiota transplantation[J].Gastroenterology,2013,145(5):946-953.
[16]	KOÇKAYA M,ÖZŞENSOY Y.Determination of some blood parameters and macro elements in coccidiosis affected akkaraman kangal lambs[J].J Asian Sci Res,2016,6(9):138-142.
[17]	SAKAI H,SAGARA A,MATSUMOTO K,et al.5-fluorouracil induces diarrhea with changes in the expression of inflammatory cytokines and aquaporins in mouse intestines[J].PLoS One,2013,8(1):e54788.
[18]	JIANG B,SNIPES-MAGALDI L,DENNEHY P,et al.Cytokines as mediators for or effectors against rotavirus disease in children[J].Clin Diagn Lab Immun,2003,10(6):995-1001.
[19]	JI J,HU S L,ZHENG M Z,et al.Bacillus amyloliquefaciens SC06 inhibits ETEC-induced pro-inflammatory responses by suppression of MAPK signaling pathways in IPEC-1 cells and diarrhea in weaned piglets[J].Livest Sci,2013,158(1-3):206-214.
[20]	NIEDERWERDER M C,HESSE R A.Swine enteric coronavirus disease:a review of 4 years with porcine epidemic diarrhoea virus and porcine deltacoronavirus in the United States and Canada[J].Transbound Emerg Dis,2018,65(3):660-675.
[21]	LEE C H,STEINER T,PETROF E O,et al.Frozen vs fresh fecal microbiota transplantation and clinical resolution of diarrhea in patients with recurrent Clostridium difficile infection:a randomized clinical trial[J].JAMA,2016,315(2):142-149.
[22]	SEKIROV I,RUSSELL S L,ANTUNES L C M,et al.Gut microbiota in health and disease[J].Physiol Rev,2010,90(3):859-904.
[23]	MAO S Y,ZHANG M L,LIU J H,et al.Characterising the bacterial microbiota across the gastrointestinal tracts of dairy cattle:membership and potential function[J].Sci Rep,2015,5:16116.
[24]	HU L S,GENG S J,LI Y,et al.Exogenous fecal microbiota transplantation from local adult pigs to crossbred newborn piglets[J].Front Microbiol,2018,8:2663.
[25]	HU J,MA L B,NIE Y F,et al.A microbiota-derived bacteriocin targets the host to confer diarrhea resistance in early-weaned piglets[J].Cell Host Microbe,2018,24(6):817-832.E8.
[26]	FERRECCHIA C E,HOBBS T R.Efficacy of oral fecal bacteriotherapy in rhesus macaques (Macaca mulatta) with chronic diarrhea[J].Comp Med,2013,63(1):71-75.

粪菌移植法治疗非特异病原性羔羊腹泻的效果初报

Preliminary Report of the Therapeutic Effect of Fecal Microbiota Transplantation on Non-specific Pathogenic Diarrhea in Suckling Lambs

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 26

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李剑南, 袁利明, 华进联. CD46基因在家畜抗病育种中的应用研究进展[J]. 畜牧兽医学报, 2024, 55(5): 1866-1874.
[2]	马茹梦, 赵玉梁, 马明爽, 国桂海, 刘芯孜, 李佳璇, 崔文, 姜艳平, 单智夫, 周晗, 王丽, 乔薪瑗, 唐丽杰, 王晓娜, 李一经. 不同猪源受体菌表达猪流行性腹泻病毒保护性抗原S1诱导免疫应答的比较研究[J]. 畜牧兽医学报, 2024, 55(5): 2090-2099.
[3]	徐红, 商红旗, 张雪, 钱嘉莉, 王传红, 宋旭, 宝梅英, 刘诗雨, 张格格, 郭容利, 赵永祥, 范宝超, 李彬. C8orf4基因编码蛋白对猪流行性腹泻病毒体外复制的抑制效应[J]. 畜牧兽医学报, 2024, 55(5): 2100-2108.
[4]	王静, 张淑娟, 胡霞, 刘向阳, 张兴翠, 宋振辉. CD44通过影响猪流行性腹泻病毒复制调节钠氢交换体3活性[J]. 畜牧兽医学报, 2024, 55(5): 2176-2185.
[5]	胡泽奇, 李润成, 谭祖明, 谢秀艳, 王江平, 秦乐娟, 李荣, 葛猛. PEDV、PoRVA和PDCoV TaqMan三重RT-qPCR检测方法的建立与初步应用[J]. 畜牧兽医学报, 2024, 55(5): 2267-2272.
[6]	林莉莉, 张梦迪, 朱琳琳, 马海龙, 孙琪, 何启盖, 张梦佳, 李文涛. 基于猪流行性腹泻病毒GⅡb亚型重组荧光病毒中和抗体检测方法的建立[J]. 畜牧兽医学报, 2024, 55(4): 1649-1660.
[7]	王潇, 张昊, 栾庆江, 李慧, 杨鼎, 王婷月, 田菁, 赵濛, 陈陆, 田如刚. 冷热应激对肉牛生理指标及基因表达影响的研究进展[J]. 畜牧兽医学报, 2024, 55(3): 894-904.
[8]	任莉鑫, 张静怡, 徐沙沙, 杨柳, 张兴翠, 宋振辉. ACE2对猪流行性腹泻病毒体外感染传代猪小肠上皮细胞的影响[J]. 畜牧兽医学报, 2024, 55(3): 1238-1248.
[9]	李思远, 付新成, 袁雪松, 毛立, 蔡旭航, 孙心如, 黄金, 谢玲玲, 王府, 周华, 张琪, 李基棕, 李彬. 河北省廊坊市牛主要病毒性腹泻病原感染状况检测及牛冠状病毒演化分析[J]. 畜牧兽医学报, 2024, 55(2): 649-659.
[10]	陈雪清, 李志强, 吴雨龙, 张崇昊, 张源淑. 临床腹泻猪空肠组织中肾素-血管紧张素系统(RAS)的表达变化及与肠道炎症的关系[J]. 畜牧兽医学报, 2024, 55(2): 751-758.
[11]	肖乐, 刘峻源, 曾雯玉, 汪芹, 韩雯珏, 刘彦泠, 范誉, 徐雨婷, 杨贝妮, 肖雄, 王自力. 基于微生物组和宿主转录组整合分析香砂六君子汤对ETEC诱导断奶腹泻仔猪回肠损伤的调控机制[J]. 畜牧兽医学报, 2024, 55(2): 797-808.
[12]	周建浩, 王东方, 刘影, 王淑娟, 马震原, 谢彩华, 赵雪丽, 杨海波, 冯桂丹, 康台生, 胡煜锋, 李博文, 闫若潜. 猪流行性腹泻病毒微滴式数字PCR定量检测方法的建立及初步应用[J]. 畜牧兽医学报, 2024, 55(1): 413-418.
[13]	姜玲玲, 牛小霞, 刘强, 张刚, 王璞, 李勇. 宁夏地区肉牛腹泻相关病毒感染状况的分析[J]. 畜牧兽医学报, 2023, 54(9): 3863-3871.
[14]	刘华, 殷冬冬, 邵颖, 宋祥军, 王振宇, 潘孝成, 涂健, 何长生, 朱良强, 祁克宗. 猪流行性腹泻病毒RAA-CRISPR/Cas13a检测方法的建立与初步应用[J]. 畜牧兽医学报, 2023, 54(9): 3991-3997.
[15]	王思盈, 邹宏, 宋振辉. Na⁺/H⁺交换体家族第三个亚型在感染性腹泻中的作用及活性调控机制[J]. 畜牧兽医学报, 2023, 54(8): 3230-3241.