CRISPR/Cas9基因编辑技术在重要猪病毒病防控中的研究与应用

doi:10.11843/j.issn.0366-6964.2020.05.006

摘要/Abstract

摘要： 规律成簇的间隔短回文重复序列系统（clustered regularly interspaced short palindromic repeat，CRISPR）是一种广泛存在于古细菌和细菌中，由RNA介导在Cas蛋白协助下发挥作用的获得性免疫系统，目前，已发现的CRISPR系统中以CRISPR/Cas9应用最为广泛，本文主要对CRISPR/Cas9系统的基本原理和研究进展进行概述，着重介绍其在重要猪病毒病防控中的应用，包括改造宿主和改造病毒两方面，该技术为研究病毒致病机制、新型疫苗研发以及抗病育种研究等提供了强有力的工具，对疫病的控制有着深远的影响。

关键词: CRISPR/Cas9, 猪病防控, 新型疫苗, 抗病育种

Abstract: Clustered regularly interspaced short palindromic repeat (CRISPR) is an acquired immune system that exists widely in archaea and bacteria, mediated by RNA and assisted by Cas protein. It has been found that the CRISPR/Cas9 was the most widely used CRISPR system. This review mainly summarizes the basic principle and research progress of the CRISPR/Cas9 system, and highlights its application in the prevention and control of important swine virus diseases, including host modification and virus modification. This technology provides a powerful tool for the study of virus pathogenic mechanism, next generation vaccine development and disease resistance breeding. It has a far-reaching impact on the control of the diseases.

Key words: CRISPR/Cas9, prevention and control of swine disease, next generation vaccine, disease resistance breeding

中图分类号:

戴学宇, 张乾义, 徐璐, 赵启祖, 王琴, 夏应菊. CRISPR/Cas9基因编辑技术在重要猪病毒病防控中的研究与应用[J]. 畜牧兽医学报, 2020, 51(5): 943-951.

DAI Xueyu, ZHANG Qianyi, XU Lu, ZHAO Qizu, WANG Qin, XIA Yingju. Research Progress and Application of CRISPR/Cas9 Gene Editing Technology in Prevention and Control of Important Swine Virus Diseases[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 943-951.

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