黑水虻幼虫粉对育肥猪盲肠食糜主要微生物数量和代谢产物的影响

doi:10.11843/j.issn.0366-6964.2020.02.011

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (2): 299-310.doi: 10.11843/j.issn.0366-6964.2020.02.011

黑水虻幼虫粉对育肥猪盲肠食糜主要微生物数量和代谢产物的影响

余苗¹, 李贞明¹, 王刚¹, 容庭¹, 陈卫东^1*, 王凤英², 马现永^1*

1. 广东省农业科学院动物科学研究所, 农业部华南动物营养与饲料重点实验室, 畜禽育种国家重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东省畜禽肉品质量安全控制与评定工程技术研究中心, 广州 510640;
2. 广州安芮洁环保科技有限公司, 广州 510080

收稿日期:2019-07-30 出版日期:2020-02-23 发布日期:2020-02-22
通讯作者: 陈卫东,主要从事生态健康养殖研究,E-mail:chenweidong@gdaas.cn;马现永,主要从事生态健康养殖研究,E-mail:maxianyong@gdaas.cn
作者简介:余苗(1985-),女,四川广安人,博士,主要从事肠道微生物与生态健康养殖研究,E-mail:ymiao5656@163.com
基金资助:
国家自然科学基金（31902200）；广东省农业科学院院长基金（201802B）；广东省农业科学院人才计划（201803）；畜禽育种与营养研究重点实验室开放运行（2017B030314044）；广东省现代农业产业技术体系创新团队（2019KJ115）；广东省科技计划项目（2019A050505007）；省级乡村振兴战略专项资金（2018kczx06）

Effects of Hermetia Illucens Larvae Meal on the Number of Main Microbes and Metabolites in the Cecal Digesta of Finishing Pigs

YU Miao¹, LI Zhenming¹, WANG Gang¹, RONG Ting¹, CHEN Weidong^1*, WANG Fengying², MA Xianyong^1*

1. Guangdong Engineering Technology Research Center of Animal Meat Quality Safety Control and Evaluation, Guangdong Public Laboratory of Animal Breeding and Nutrition, State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Guangzhou AnRuiJie Environmental Protection Technology Co., Ltd., Guangzhou 510080, China

Received:2019-07-30 Online:2020-02-23 Published:2020-02-22

摘要/Abstract

摘要： 旨在研究日粮中添加不同比例的黑水虻幼虫粉对育肥猪盲肠食糜主要微生物数量和代谢产物的影响。本试验选取72头130日龄、健康且初始体重相近（（76.0±0.56）kg）的杜×长×大三元杂交猪，随机分为3个处理组，每组8个重复，每个重复3头猪。对照组饲喂基础日粮，试验组分别饲喂含有4%（4%组）和8%（8%组）的黑水虻幼虫粉，通过调整日粮配方，3组日粮的蛋白含量和代谢能一致。于正式试验的第42天，每个重复选取1头体重接近平均体重的猪进行屠宰，取盲肠食糜用于相关指标的测定。结果表明：1）与对照组相比，日粮添加4%的黑水虻幼虫粉显著增加了盲肠食糜Bifidobacterium、Lactobacillus、Prevotella、Clostridium cluster IV和Roseburia的数量（P<0.05），而降低了Escherichia coli的数量（P<0.05）；日粮添加8%的黑水虻幼虫粉显著增加了Prevotella和Bifidobacterium的数量（P<0.05）。2）与对照组相比，日粮添加4%和8%的黑水虻幼虫粉显著增加了盲肠食糜中乳酸、总短链脂肪酸和乙酸的浓度（P<0.05），同时，日粮添加8%黑水虻幼虫粉还降低了戊酸的浓度（P<0.05）；对于氮代谢产物而言，与对照组相比，日粮添加4%的黑水虻幼虫粉显著降低了盲肠食糜中总生物胺、尸胺、腐胺、精胺、对甲酚和苯酚的浓度（P<0.05），而日粮添加8%的黑水虻幼虫粉显著降低了尸胺和苯酚的浓度（P<0.05）。综上所述，本试验条件下，在日粮中添加4%的黑水虻幼虫粉可增加育肥猪盲肠食糜中部分有益微生物的数量和有益代谢物的浓度，降低潜在致病菌的数量和氮代谢化合物的浓度，表明一定比例的黑水虻幼虫粉可作为优质的蛋白质饲料原料应用于养猪生产中。

关键词: 黑水虻幼虫粉, 猪, 盲肠, 肠道微生物, 代谢产物

Abstract: This study aimed to investigate the effects of Hermetia illucens larvae meal on the number of main microbes and metabolites in the cecal digesta of finishing pigs. Seventy-two 130 days of age healthy (Duroc×Landrace×Large White) crossbred pigs with similar body weight of (76.0±0.56) kg were randomly allocated to 3 different dietary treatments with 8 replicates for each treatment and 3 pigs for each replicate. Pigs in the control group were fed basal diets, pigs in the experimental groups were fed diets with 4% (4% group) and 8% (8% group) Hermetia illucens larvae meal to attain the same nitrogen and energy level, respectively by adjusting diet formula. After 42 days formal feeding, one pig from each replicate in each group was randomly selected and slaughtered, and the cecal digesta were collected for correlated parameters analysis. The results showed that:1) Compared with control group, dietary with 4% Hermetia illucens larvae meal significantly increased the number of Bifidobacterium, Lactobacillus, Prevotella, Clostridium cluster IV, and Roseburia in the cecal digesta of pigs (P<0.05), while significantly decreased the number of Escherichia coli (P<0.05); Meanwhile, dietary with 8% Hermetia illucens larvae meal significantly increased the number of Prevotella and Bifidobacterium in the cecal digesta of pigs (P<0.05). 2) Compared with control group, dietary with 4% and 8% Hermetia illucens larvae meal significantly increased the concentrations of lactate, total short-chain fatty acid, and acetate in the cecal digesta of pigs (P<0.05), and dietary with 8% Hermetia illucens larvae meal significantly decreased the concentration of valerate (P<0.05). For nitrogen metabolites, compared with control group, dietary with 4% Hermetia illucens larvae meal significantly decreased the concentrations of total amines, cadaverine, putrescine, spermine, p-cresol, and phenol in the cecal digesta of pigs (P<0.05), and dietary with 8% Hermetia illucens larvae meal also significantly decreased the cadaverine and phenol (P<0.05). In conclusion, under the conditions of this trail, dietary with 4% Hermetia illucens larvae meal increased the number of beneficial microbes and the concentrations of beneficial metabolites in the cecal digesta of finishing pigs, while decreased the number of potential pathogenic microbes and the concentrations of nitrogen metabolites. These findings indicated that appropriate proportion of Hermetia illucens larvae meal could be used as high quality protein feedstuff in pig production.

Key words: Hermetia illucens larvae meal, pig, cecum, intestinal microbe, metabolites

中图分类号:

S828.5

余苗, 李贞明, 王刚, 容庭, 陈卫东, 王凤英, 马现永. 黑水虻幼虫粉对育肥猪盲肠食糜主要微生物数量和代谢产物的影响[J]. 畜牧兽医学报, 2020, 51(2): 299-310.

YU Miao, LI Zhenming, WANG Gang, RONG Ting, CHEN Weidong, WANG Fengying, MA Xianyong. Effects of Hermetia Illucens Larvae Meal on the Number of Main Microbes and Metabolites in the Cecal Digesta of Finishing Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2): 299-310.

参考文献 36

[1]	中国产业信息网.2018年中国对美农产品进出口贸易额分析[EB/OL].(2018-12-05)[2018-04-28].http://www.chyxx.com/industry/201804/635390.html. China Animal Husbandry Information Network.Analysis of China's import and export trade volume of agricultural products to the United States in 2018[EB/OL].(2018-12-05)[2018-04-28].http://www.chyxx.com/industry/201804/635390.html.(in Chinese)
[2]	VAN HUIS A.Potential of insects as food and feed in assuring food security[J].Annu Rev Entomol,2013,58:563-583.
[3]	CUTRIGNELLI M I,MESSINA M,TULLI F,et al.Evaluation of an insect meal of the Black Soldier Fly (Hermetia illucens) as soybean substitute:intestinal morphometry,enzymatic and microbial activity in laying hens[J].Res Vet Sci,2018,117:209-215.
[4]	MAKKAR H P S,TRAN G,HEUZÉ V,et al.State-of-the-art on use of insects as animal feed[J].Anim Feed Sci Technol,2014,197:1-33.
[5]	朱伟云,余凯凡,慕春龙,等.猪的肠道微生物与宿主营养代谢[J].动物营养学报,2014,26(10):3046-3051.ZU W Y,YU K F,MU C L,et al.Gut microbiota and host nutrition metabolism in pigs[J].Chinese Journal of Animal Nutrition,2014,26(10):3046-3051.(in Chinese)
[6]	BORRELLI L,CORETTI L,DIPINETO L,et al.Insect-based diet,a promising nutritional source,modulates gut microbiota composition and SCFAs production in laying hens[J].Sci Rep,2017,7(1):16269.
[7]	YU M,LI Z M,CHEN W D,et al.Use of Hermetia illucens larvae as a dietary protein source:effects on growth performance,carcass traits,and meat quality in finishing pigs[J].Meat Sci,2019,158:107837.
[8]	余苗,李贞明,陈卫东,等.黑水虻幼虫粉对育肥猪营养物质消化率、血清生化指标和氨基酸组成的影响[J].动物营养学报,2019,31(7):3330-3337.Y M,LI Z M,CHEN W D,et al.Effects of Hermetia illucens L.larvae meal on nutrient digestibility,serum biochemical indices and amino acid composition of finishing pigs[J].Chinese Journal of Animal Nutrition,2019,31(7):3330-3337.(in Chinese)
[9]	YU M,LI Z M,CHEN W D,et al.Hermetia illucens larvae as a potential dietary protein source altered the microbiota and modulated mucosal immune status in the colon of finishing pigs[J].J Anim Sci Biotechnol,2019,10(1):50.
[10]	National Research Council.Nutrient requirements of swine[M].11th ed.Washington,DC:National Academies Press,2012.
[11]	YU M,ZHANG C J,YANG Y X,et al.Long-term effects of early antibiotic intervention on blood parameters,apparent nutrient digestibility,and fecal microbial fermentation profile in pigs with different dietary protein levels[J].J Anim Sci Biotechnol, 2017,8(1):60.
[12]	CHANEY A L,MARBACH E P.Modified reagents for determination of urea and ammonia[J].Clin Chem,1962,8(2):130-132.
[13]	YANG Y X,MU C L,ZHANG J F,et al.Determination of biogenic amines in digesta by high performance liquid chromatography with precolumn dansylation[J].Anal Lett,2014,47(8):1290-1298.
[14]	SUZUKI M T,TAYLOR L T,DELONG E F.Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5'-nuclease assays[J].Appl Environ Microbiol,2000,66(11):4605-4614.
[15]	GUO X,XIA X,TANG R,et al.Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs[J].Lett Appl Microbiol,2008,47(5):367-373.
[16]	HUIJSDENS X W,LINSKENS R K,MAK M,et al.Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR[J].J Clin Microbiol,2002,40(12):4423-4427.
[17]	LAN Y,XUN S,TAMMINGA S,et al.Real-Time PCR detection of lactic acid bacteria in cecal contents of Eimeria tenella-Lnfected broilers fed soybean oligosaccharides and soluble soybean polysaccharides[J].Poult Sci,2004,83(10):1696-1702.
[18]	WALKER A W,INCE J,DUNCAN S H,et al.Dominant and diet-responsive groups of bacteria within the human colonic microbiota[J].ISME J,2011,5(2):220-230.
[19]	MATSUKI T,WATANABE K,FUJIMOTO J,et al.Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces[J].Appl Environ Microbiol,2004,70(12):7220-7228.
[20]	BARTOSCH S,FITE A,MACFARLANE G T,et al.Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota[J].Appl Environ Microbiol,2004,70(6):3575-3581.
[21]	VERMA R,VERMA A K,AHUJA V,et al.Real-time analysis of mucosal flora in patients with inflammatory bowel disease in India[J].J Clin Microbiol,2010,48(11):4279-4282.
[22]	LAYTON A,MCKAY L,WILLIAMS D,et al.Development of bacteroides 16S rRNA Gene TaqMan-based real-time PCR assays for estimation of total,human,and bovine fecal pollution in water[J].Appl Environ Microbiol,2006,72(6):4214-4224.
[23]	WALKER A W,DUNCAN S H,LEITCH E C M,et al.pH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon[J].Appl Environ Microbiol,2005, 71(7):3692-3700.
[24]	CULLERE M,TASONIERO G,GIACCONE V,et al.Black soldier fly as dietary protein source for broiler quails:apparent digestibility,excreta microbial load,feed choice,performance,carcass and meat traits[J].Animal,2016,10(12):1923-1930.
[25]	WANG W,CHEN L P,ZHOU R,et al.Increased proportions of Bifidobacterium and the Lactobacillus group and loss of butyrate-producing bacteria in inflammatory bowel disease[J].J Clin Microbiol,2014,52(2):398-406.
[26]	LOUIS P,HOLD G L,FLINT H J.The gut microbiota,bacterial metabolites and colorectal cancer[J].Nat Rev Microbiol,2014, 12(10):661-672.
[27]	BARNICH N,CARVALHO F A,GLASSER A L,et al.CEACAM6 acts as a receptor for adherent-invasive E. coli,supporting ileal mucosa colonization in Crohn disease[J].J Clin Invest,2007,117(6):1566-1574.
[28]	TERADA A,HARA H,SATO D,et al.Effect of dietary chitosan on faecal microbiota and faecal metabolites of humans[J].Microb Ecol Health Dis,1995,8(1):15-21.
[29]	NEYRINCK A M,POSSEMIERS S,VERSTRAETE W,et al.Dietary modulation of clostridial cluster XIVa gut bacteria (Roseburia spp.) by chitin-glucan fiber improves host metabolic alterations induced by high-fat diet in mice[J].J Nutr Biochem,2012,23(1):51-59.
[30]	DECUYPERE J A,DIERICK N A.The combined use of triacylglycerols containing medium-chain fatty acids and exogenous lipolytic enzymes as an alternative to in-feed antibiotics in piglets:concept,possibilities and limitations.An overview[J].Nutr Res Rev,2003,16(2):193-210.
[31]	YI H Y,CHOWDHURY M,HUANG Y D,et al.Insect antimicrobial peptides and their applications[J].Appl Microbiol Biotechnol,2014,98(13):5807-5822.
[32]	GAO K,PI Y,PENG Y,et al.Time-course responses of ileal and fecal microbiota and metabolite profiles to antibiotics in cannulated pigs[J].Appl Microbiol Biotechnol,2018,102(5):2289-2299.
[33]	TAN J,MCKENZIE C,VUILLERMIN P J,et al.Dietary fiber and bacterial SCFA enhance oral tolerance and protect against food allergy through diverse cellular pathways[J].Cell Rep,2016,15(12):2809-2824.
[34]	TREMAROLI V,BÄCKHED F.Functional interactions between the gut microbiota and host metabolism[J].Nature, 2012, 489(7415):242-249.
[35]	RIST V T S,WEISS E,EKLUND M,et al.Impact of dietary protein on microbiota composition and activity in the gastrointestinal tract of piglets in relation to gut health:a review[J].Animal,2013,7(7):1067-1078.
[36]	DAVILA A M,BLACHIER F,GOTTELAND M,et al.Re-print of "intestinal luminal nitrogen metabolism:role of the gut microbiota and consequences for the host"[J].Pharmacol Res,2013,69(1):114-126.

黑水虻幼虫粉对育肥猪盲肠食糜主要微生物数量和代谢产物的影响

Effects of Hermetia Illucens Larvae Meal on the Number of Main Microbes and Metabolites in the Cecal Digesta of Finishing Pigs

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