miR-502在犬乳腺癌中的表达及意义

doi:10.11843/j.issn.0366-6964.2020.01.022

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (1): 193-197.doi: 10.11843/j.issn.0366-6964.2020.01.022

miR-502在犬乳腺癌中的表达及意义

任晓丽¹, 范玉营², 石冬梅¹, 田传文¹, 卢水龙¹, 刘云^2*

1. 河南牧业经济学院动物医药学院, 郑州 450046;
2. 东北农业大学动物医学学院, 哈尔滨 150030

收稿日期:2019-08-23 出版日期:2020-01-23 发布日期:2020-01-17
通讯作者: 刘云,主要从事临床兽医学研究,E-mail:liuyun@neau.edu.cn
作者简介:任晓丽(1983-),女,河南安阳人,讲师,博士,主要从事小动物肿瘤学的研究,E-mail:renxiaoli168@163.com
基金资助:
“十三五”国家重点研发项目（2016YFD0501008）；河南省级高等学校重点项目（20B230004）；河南牧业经济学院院级重点培育学科建设项目（41000002）

Expressions of miR-502 in Canine Breast Cancer and Clinical Significance

REN Xiaoli¹, FAN Yuying², SHI Dongmei¹, TIAN Chuanwen¹, LU Shuilong¹, LIU Yun^2*

1. College of Veterinary Medicine, Henan University of Animal Husbandry of Economy, Zhengzhou 450046, China;
2. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

Received:2019-08-23 Online:2020-01-23 Published:2020-01-17

摘要/Abstract

摘要： 旨在研究微小miR-502（cfa-miRNA-502）在犬乳腺癌组织中的表达情况及其临床意义。采用实时定量PCR方法检测30例犬乳腺癌组织及癌旁正常组织中miR-502的表达水平，分析miR-502表达与犬乳腺癌组织临床病理特征（年龄、肿瘤大小、组织学分级和转移）的关系。结果显示：与癌旁组织相比，犬乳腺癌组织中miR-502的表达水平显著升高，差异具有统计学意义（P<0.01）；miR-502表达与犬乳腺癌的组织学分级与转移有关（P<0.05），但与年龄和肿瘤大小无关（P>0.05）。本研究提示miR-502在犬乳腺癌组织中表达上调，其表达与犬乳腺肿瘤的发生、发展有一定关系，miR-502可能作为犬乳腺癌诊断的肿瘤标记物。

关键词: 乳腺癌, 犬, miR-502, 基因表达

Abstract: The aim of this study was to investigate the expression level of miR-502 and its clinical significance in canine breast cancer tissues. A total of 30 cases of canine breast cancer tissues and normal adjacent tissues were enrolled in this study. The expression levels of miR-502 were detected by real-time quantitative polymerase chain reaction (qRT-PCR). The relationship between the levels of miR-502 and the clinicopathological features (age, tumor size, histological grade and metastasis state) of canine breast cancer were analyzed. The experiment results showed that compared with the adjacent normal tissues, the levels of miR-502 transcription in canine breast cancer tissues increased significantly (P<0.01). The transcription of miR-502 was related with histological grade and metastasis in canine breast cancer (P<0.05); but not related with tumor size or age (P>0.05). The miR-502 transcription level is up-regulated in canine breast cancer that related with tumorigenesis and progression of canine breast cancer. miR-502 can be used as a tumor marker for diagnosis prediction of canine breast cancer.

Key words: breast cancer, canine, miR-502, gene expression

中图分类号:

S857.4

任晓丽, 范玉营, 石冬梅, 田传文, 卢水龙, 刘云. miR-502在犬乳腺癌中的表达及意义[J]. 畜牧兽医学报, 2020, 51(1): 193-197.

REN Xiaoli, FAN Yuying, SHI Dongmei, TIAN Chuanwen, LU Shuilong, LIU Yun. Expressions of miR-502 in Canine Breast Cancer and Clinical Significance[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 193-197.

参考文献 15

[1]	PINHO S S, CARVALHO S, CABRAL J, et al. Canine tumors:a spontaneous animal model of human carcinogenesis[J]. Transl Res, 2012, 159(3):165-172.
[2]	NGUYEN F, PEÑA L, IBISCH C, et al. Canine invasive mammary carcinomas as models of human breast cancer. Part 1:natural history and prognostic factors[J]. Breast Cancer Res Treat, 2018, 167(3):635-648.
[3]	ABBA M L, PATIL N, LEUPOLD J H, et al. MicroRNA regulation of epithelial to mesenchymal transition[J]. J Clin Med, 2016, 5(1):8.
[4]	IORIO M V, CROCE C M. MicroRNA dysregulation in cancer:diagnostics, monitoring and therapeutics. A comprehensive review[J]. EMBO Mol Med, 2012, 4(3):143-159.
[5]	CZECH B, HANNON G J. Small RNA sorting:matchmaking for Argonautes[J]. Nat Rev Genet, 2011, 12(1):19-31.
[6]	LIU B, ZHANG X N, SONG F J, et al. MiR-502/SET8 regulatory circuit in pathobiology of breast cancer[J]. Cancer Lett, 2016, 376(2):259-267.
[7]	MISDORP W, ELSE R W, HELLMEN E, et al. Histological classification of mammary tumors of the dog and the cat[M]//SHULMAN F I. World Health Organization International Histological Classification Tumors of Domestic Animals. 2nd ed. Washington:Armed Forces Institute of Pathology, 1999.
[8]	ELSTON C W, ELLIS I O. Assessment of histological grade[M]//ELSTON C W, ELLIS I O. The Breast. New York:Churchill Livingstone, 1998.
[9]	SIEGEL R L, MILLER K D, JEMAL A. Cancer statistics, 2019[J]. CA Cancer J Clin, 2019, 69(1):7-34.
[10]	BERTOLI G, CAVA C, CASTIGLIONI I. The potential of miRNAs for diagnosis, treatment and monitoring of breast cancer[J]. Scand J Clin Lab Invest, 2016, 76(S245):S34-S39.
[11]	SUN L L, WANG J, ZHAO Z J, et al. Suppressive role of miR-502-5p in breast cancer via downregulation of TRAF2[J]. Oncol Rep, 2014, 31(5):2085-2092.
[12]	CHEN S L, LI F, CHAI H Y, et al. miR-502 inhibits cell proliferation and tumor growth in hepatocellular carcinoma through suppressing phosphoinositide 3-kinase catalytic subunit gamma[J]. Biochem Biophys Res Commun, 2015, 464(2):500-505.
[13]	JIN H S, YU M, LIN Y, et al. MiR-502-3P suppresses cell proliferation, migration, and invasion in hepatocellular carcinoma by targeting SET[J]. Onco Targets Ther, 2016, 9:3281-3289.
[14]	XU J, PAN X X, HU Z D. MiR-502 mediates esophageal cancer cell TE1 proliferation by promoting AKT phosphorylation[J]. Biochem Biophys Res Commun, 2018, 501(1):119-123.
[15]	OSAKI T, SUNDEN Y, SUGIYAMA A, et al. Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression[J]. J Vet Sci, 2016, 17(3):385-390.

miR-502在犬乳腺癌中的表达及意义

Expressions of miR-502 in Canine Breast Cancer and Clinical Significance

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