T-2毒素对BALB/c小鼠营养物质表观消化率及小肠形态结构的影响

doi:10.11843/j.issn.0366-6964.2015.09.013

摘要/Abstract

摘要：

本试验旨在研究T-2毒素对BALB/c小鼠粪便pH、水分以及肠道营养物质、矿物元素、氨基酸表观消化率和小肠形态结构的影响。选用平均体重为(20±2) g的BALB/c小鼠80只，随机分成4组，每组20只。4组分别为0（对照组）、0.4、1.0和2.5 mg•kg^-1•BW T-2 毒素剂量组，连续灌胃28 d。结果表明，1.0 mg•kg^-1•BW组小鼠粪便pH显著高于对照组和0.4 mg•kg^-1•BW组（P<0.05），2.5 mg•kg^-1•BW组则显著高于前3组（P<0.05或P<0.01）。粗蛋白、灰分、粗纤维和粗脂肪的表观消化率随T-2毒素添加量的增加而降低，不同处理组之间统计学差异均显著（P<0.05或P<0.01）。其次，与对照组相比，0.4 mg•kg^-1•BW组矿物元素Ca、Fe、Mg、Na和P的消化率显著降低（P<0.01）；1.0 和2.5 mg•kg^-1•BW组Ca、Zn、Mg、K、Mn和P的消化率低于对照组和0.4 mg•kg^-1•BW组（P<0.05或P<0.01），2.5 mg•kg^-1•BW组显著低于1.0 mg•kg^-1•BW组（P<0.01）。此外，1.0 mg•kg^-1•BW组氨基酸Asp、Thr、Ser、Glu、Gly、Val、Ile、His、Ala、Leu、Phe、Lys、Arg和Pro的消化率显著低于对照组或0.4 mg•kg^-1•BW组（P<0.05或P<0.01）；2.5 mg•kg^-1•BW组所有氨基酸指标均显著低于其他组（P<0.01）。同时，T-2的剂量超过1.0 mg•kg^-1•BW时，小鼠肠道形态结构发生严重损伤，黏膜脱落、绒毛数量减少，与对照组和0.4 mg•kg^-1•BW组相比，十二指肠、空肠和回肠均出现绒毛数量减少、长度降低、隐窝深度增加、绒毛长度/隐窝深度（V/C）比值降低，统计学差异显著（P<0.05或P<0.01）。由此可知，T-2毒素处理使肠道黏膜损伤，肠道pH升高，营养物质、矿物元素和氨基酸的表观消化率降低，且这种损伤和降低呈剂量效应关系。

Abstract:

This study was conducted to investigate the effect of T-2 toxin exposed to BALB/c mice on pH and moisture content in feces，apparent digestibility of nutrients，mineral elements and amino acids，as well as morphological structure in small intestine.A total of 80 BALB/c mice weighted about(20±2) g were randomly allotted to 4 groups(20 mice per group).The mice were fed one of four kinds of diets(0，0.4，1.0 and 2.5 mg•kg^-1•BW T-2 toxin exposed daily via intragastric administration for 28 d).The results showed as follows：the pH in feces in 1.0 mg•kg^-1•BW group was higher than that in control and 0.4 mg•kg^-1•BW groups(P<0.05)，pH in feces in 2.5 mg•kg^-1•BW group was the highest among all 4 groups(P<0.05 or P<0.01).The apparent digestibility of crude protein，ash，crude fiber and ether extract were greatly decreased with the increasing of T-2 toxin addition，and there were significant difference among different treatment groups(P<0.05 or P<0.01).The apparent digestibility of mineral elements(including Ca，Fe，Mg，Na and P) of 0.4 mg•kg^-1•BW group was largely reduced compared with control group(P<0.01).The mineral elements(including Ca，Zn，Mg，K，Mn and P) in 1.0 and 2.5 mg•kg^-1•BW groups were lower than those of control and 0.4 mg•kg^-1•BW groups(P>0.05 and P<0.01)，and 2.5 mg•kg^-1•BW group was significantly lower than 1.0 mg•kg^-1•BW group(P<0.01).The apparent digestibility of amino acid(including Asp，Thr，Ser，Glu，Gly，Ala，Val，Ile，Leu，Phe，Lys，His，Arg and Pro) in 1.0 mg•kg^-1•BW group were also observably declined compared with control or 0.4 mg•kg^-1•BW groups(P<0.05 and P<0.01)，and the digestibility of all kinds of amino acid in 2.5 mg•kg^-1•BW group were the lowest among all the 4 groups(P<0.01).Moreover，the duodenum，jejunum and ileum presented mucosal damage and microvilli exfoliated when the dose of T-2 toxin was more than 1.0 mg•kg^-1•BW.In addition，reduction of villous number and villous length，increase of crypt depth，decrease of the ratio of villus length to crypt depth were also observed in 1.0 and 2.5 mg•kg^-1•BW groups，and there were significant differences between 1.0,2.5 mg•kg^-1•BW groups,and control,0.4 mg•kg^-1•BW groups(P<0.05 and P<0.01)，respectively.It was concluded that the supplementation of T-2 toxin could damage intestinal mucosal，induce the increase of pH，decrease the apparent digestibility of nutrient，mineral elements and amino acid，and all these damage and decreasing were associated with dose-effect relationship of T-2 toxin.

中图分类号:

S815

杨俊花，陈慧英，韩薇，赵志辉，孙真真，郭文博. T-2毒素对BALB/c小鼠营养物质表观消化率及小肠形态结构的影响[J]. 畜牧兽医学报, 2015, 46(9): 1584-1592.

YANG Jun-hua，CHEN Hui-ying，HAN Wei，ZHAO Zhi-hui，SUN Zhen-zhen，GUO Wen-bo. Influence of T-2 Toxin on Nutrient Apparent Digestibility and Small Intestinal Morphology in BALB/c Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1584-1592.

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参考文献

［1］UENO Y.Toxicological features of T-2 toxin and related trichothecenes［J］.Fund Appl Toxicol，1984，2(2)：S124-S132.
［2］PARENT M D.Haematotoxicity of trichothecenes［J］.Toxicol Lett，2004，153(1)：75-81.
［3］ROTTER B A，PRELUSKY D B，THOMPSON B K.The role of tryptophan in DON induced feed rejection［J］.J Environ Sci Health B，1996，31(6)：1279-1288.
［4］PESTKA J J，SMOLINSKI A T.Deoxynivalenol：toxicology and potential effects on humans［J］. J Toxicol Environ Health B Crit Rev，2005，8(1)：39-69.
［5］OBREMSKI K，ZIELONKA L，GAJECKA M，et al.Histological estimation of the small intestine wall after administration of feed containing deoxynivalenol，T-2 toxin and zearalenone in the pig［J］.Pol J Vet Sci，2008，11(4)：339-345.
［6］SKLAN D，SHELLY M，MAKOVSKY B，et al.The effect of chronic feeding of diacetoxyscirpenol and T-2 toxin on performance，health，small intestinal physiology and antibody production in turkey poults［J］.Br Poult Sci，2003，44(1)：46-52.
［7］陈国营，詹凯，朱由彩，等.枯草芽孢杆菌及其发酵豆粕对蛋鸡肠道菌群和粪便中N、S含量的影响［J］.中国家禽，2012，34(6)：10-15.
CHEN G Y，ZHAN K，ZHU Y C，et al.Effects of bacillus subtilis and fermented soybean meal on intestinal microflora，fecal nitrogen and sulfur content in layers［J］.China Poultry，2012，34(6)：10-15.(in Chinese)
［8］侯振平，印遇龙，王文杰，等.乳铁蛋白素B和天蚕素P1对投喂大肠杆菌断奶仔猪生长及肠道微生物区系的影响［J］.动物营养学报，2011，23(9)：1536-1544.
HOU Z P，YIN Y L，WANG W J，et al.Effects of lactoferricin b and cecropin p1 on growth and gut microflora in weaned piglets challenged with enterotoxigenic Escherichia coli ［J］.Chinese Journal of Animal Nutrition，2011，23(9)：1536-1544.(in Chinese)
［9］张丽英.饲料分析及饲料质量检测技术［M］.北京：中国农业大学出版社，2003.
ZHANG L Y.Feed analysis and determining technique［M］.Beijing：Chinese Agricultural University Press，2003.(in Chinese)
［10］辛国省，李爱华，巫亮，等.微波消解ICP-AES法测定配合饲料中无机元素［J］.草业科学，2011，28(7)：1384-1386.
XIN G S，LI A H，WU L，et al.Determination of inorganic elements in formula feed by microwave digestion ICP-AES［J］.Pratacultural Science，2011，28(7)：1384-1386.(in Chinese)
［11］田玉旺，李琳，朱红艳，等.提高冷冻切片制片质量的方法探讨［J］.中国组织化学与细胞化学杂志，2010，19(4)：408-410.
TIAN Y W，LI L，ZHU H Y，et al.Discussion on a method for improvement of preparation for frozen sections［J］. Chinese Journal of Histochemistry and Cytochemistry，2010，19(4)：408-410.(in Chinese)
［12］赵国先，宋海彬，马可为，等.葡萄糖氧化酶制剂对肉鸡肠道pH及盲肠微生物的影响［J］.河北农业大学学报，2009，32(4)：83-87.
ZHAO G X，SONG H B，MA K W，et al.Effects of glucose oxidase on intestinal tract pH and cecum microorganism in broiler chickens［J］.Journal of Agricultural University of Hebei，2009，32(4)：83-87.(in Chinese)
［13］KUMAGAI S，SHIMIZU T.Effects of fusarenon-X and T-2 toxin on intestinal absorption of monosaccharide in rats［J］.Arch Toxicoly，1988，61(6)：489-495.
［14］HUNDER G，SCHUMANN K，STRUGALA G，et al.Influence of subchronic exposure to low dietary deoxynivalenol，a trichothecene mycotoxin，on intestinal absorption of nutrients in mice［J］.Food Chem Toxicol，1991，29(12)：809-814.
［15］AWAD W A，BÖHM J，RAZZAZI-FAZELI E，et al.In vitro effects of deoxynivalenol on electrical properties of intestinal mucosa of laying hens［J］.Poult Sci，2005，84(6)：921-927.
［16］AWAD W A，REHMAN H，BÖHM J，et al.Effects of luminal deoxynivalenol and L-proline on electrophysiological parameters in the jejunums of laying hens［J］.Poult Sci， 2005，84(6)：928-932.
［17］CHEN L，LI P，WANG J，et al.Catabolism of nutritionally essential amino acids in developing porcine enterocytes［J］.Amino Acids，2009，37(1)：143-152.
［18］DAI Z L，ZHANG J，WU G，et al.Utilization of amino acids by bacteria from the pig small intestine［J］.Amino Acids，2010，39(5)：1201-1215.
［19］邓波，万晶，徐子伟，等.脱氧雪腐镰刀菌烯醇吸附剂对断奶仔猪生长性能、血清生化指标及肠道形态的影响［J］.动物营养学报，2014，26(5)：1294-1301.
DENG B，WAN J，XU Z W，et al.Effects of deoxynivalenol adsorbent on growth performance，serum biochemical indexes and intestinal morphology of weaner piglets［J］.Chinese Journal of Animal Nutrition，2014，26(5)：1294-1301.(in Chinese)
［20］ADEMOYERO A A，HAMILTON P B.Mouth lesions in broiler chickens caused by scirpenol mycotoxins［J］.Poult Sci，1991，70(10)：2082-2091.
［21］ADEMOYERO A A，HAMILTON P B.Influence of degree of acetylation of scirpenol mycotoxins on feed refusal by chickens［J］.Poult Sci，1989，68(6)：854-856.
［22］KONJEVIC＇ D，SREBOCAN E，GUDAN A，et al.A pathological condition possibly caused by spontaneous trichotecene poisoning in Brahma poultry：first report［J］.Avian Pathol，2004，33(3)：377-380.

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