ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (1): 157-163.doi: 10.11843/j.issn.0366-6964.2018.01.018

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Changes of Lymphocytes Proliferative Function and Cell Cycle Related Factors Expression in Peripheral Blood of SPF Chicks Infected with Reticuloendotheliosis Virus

WANG Xiao-yan, LIU Wen-chao, FU Li-sheng, GAO Xue-li, LIU Chao-nan, LÜ Xiao-ping, ZHENG Shi-min*   

  1. Heilongjiang Key Laboratory of Laboratory Animal and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
  • Received:2017-07-27 Online:2018-01-23 Published:2018-01-18

Abstract:

This experiment was conducted to explore the proliferation function of T, B lymphocytes and expression of Cyclin D1 and p27 in peripheral blood of SPF chicks infected with reticuloendotheliosis virus (REV). Eighty 1-day-old SPF chicks were randomly divided into REV infection group and control group. The T, B lymphocytes proliferation function and expression of Cyclin D1 at mRNA and protein level, transcription of p27 in peripheral blood were detected by CCK-8, RT-qPCR and Western Blot. The results showed that the proliferation ability of T and B lymphocytes in peripheral blood of SPF chicks infected with REV early was significantly lower than that of the control chicks, and the transcription of p27 mRNA was significantly higher than that of the control chicks. The expression of Cyclin D1 mRNA and protein content in the late stage of infection were significantly higher than that of the control chicks (P<0.05), but the transcription of p27 mRNA were significantly lower than those of control chicks (P<0.05). The above results showed that the decrease of T, B lymphocytes proliferative function and p27 mRNA transcription, and increase of Cyclin D1 at mRNA and protein level in blood of SPF chicks were closely related to the decrease in immune function of chicks infected with REV.

Key words: SPF chick, reticuloendotheliosis virus, Cyclin D1, proliferative function of lymphocytes, peripheral blood

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