捻转血矛线虫半胱氨酸蛋白酶对山羊PBMCs免疫功能的影响

doi:10.11843/j.issn.0366-6964.2018.04.018

Abstract

Abstract:

The present study was designed to investigate the effects of Haemonchus contortus Cysteine protease on immune functions of peripheral blood mononuclear cells (PBMCs) of goat. PBMCs were separated from goat blood sample, monocytes were isolated from PBMCs because of their adherent growth. Recombinant protein of different concentrations (0, 10, 20, 40 μg·mL^-1) was cultured with PBMCs or monocytes in vitro. Nitric oxide (NO) secretion was measured by nitrate reductase assay. Cell migration was tested by Cell Culture Inserts. The mRNA transcriptional levels of IL-2, IL-4, IL-10, IL-17, IFN-γ and TGF-β were detected by real-time fluorescent quantitative PCR. Then cell phagocytosis and apoptosis were calculated by flow cytometry. The result revealed that Hc58 was able to dramatically enhance NO release and phagocytosis of monocytes. Hc58 might significantly up-regulate the production of IL-2, IL-4, IL-17 and IFN-γ. Furthermore, it also could notably increase apoptosis ratio and migration of PBMCs. There were signs that Haemonchus contortus Cysteine protease impacted on the immune functions of goat PBMCs via different pathways.

CLC Number:

S852.73

HU Meng-juan, ZHOU Li-na, NIU Yan-ping, XU Li-xin, SONG Xiao-kai, LI Xiang-rui, YAN Ruo-feng. Effects of Haemonchus contortus Cysteine Protease on Immune Functions of Goat PBMCs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 804-810.

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Effects of Haemonchus contortus Cysteine Protease on Immune Functions of Goat PBMCs

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