捻转血矛线虫NADH:泛醌氧化还原酶结构域包含蛋白基因的克隆表达及功能分析

doi:10.11843/j.issn.0366-6964.2017.04.016

Abstract

Abstract:

The gene of NADH:ubiquinone oxidoreductase domain containing protein (NDUDC) of Haemonchus contortus was selected to clone, express, and the functions of this protein were studied in this paper. The gene of NDUDC was cloned by RT-PCR, then expression vector was constructed to obtain the recombinant protein. The antigenicity of the protein was analyzed by Western blot. Peripheral blood mononuclear cells (PBMCs) were separated from goat and cultured in vitro with the recombinant NDUDC to check the binding of the protein to PBMCs by indirect immunofluorescence assay. Different concentrations (0, 10, 20, 40 μg·mL^-1) of the recombinant protein were used to stimulate PBMCs, and the effects of cell proliferation, cell migration and NO secretion were tested. The results showed that the recombinant protein NDUDC could stimulate cell proliferation effectively, also significantly enhance cell migration and increase the secretion of NO. These results indicate that NDUDC protein has strong antigenicity, and can stimulate cells to exert immune function.

CLC Number:

S852.731

WU Ling-yan, WANG Yu-jian, WEN Yu-ling, YAN Ruo-feng, XU Li-xin, SONG Xiao-kai, LI Xiang-rui. Cloning, Expression and Function Analysis of NADH: Ubiquinone Oxidoreductase Domain Containing Protein in Haemonchus contortus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 722-730.

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Cloning, Expression and Function Analysis of NADH: Ubiquinone Oxidoreductase Domain Containing Protein in Haemonchus contortus

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