冷诱导RNA结合蛋白通过激活NF-κB信号通路影响H1N1甲型流感病毒的复制

doi:10.11843/j.issn.0366-6964.2016.10.020

Abstract

Abstract:

The cold-inducible RNA-binding protein (CIRP) is an upstream regulator of the NF-κB and ERK pathways，which are essential for the replication of the influenza virus and the initial immune response.In order to investigate the effect of CIRP on the replication of H1N1 influenza A virus and its possible molecular mechanism，in this study，CIRP overexpression BHK-21 (Cirp+BHK-21) cells were constructed，and the phosphorylation levels of NF-κB and ERK in Cirp+BHK-21 cells were detected by Western blot to confirm the effect of CIRP on regulation of NF-κB and ERK1/2；Real-time RT-PCR was used to detect the dynamic changes of virus load in Cirp+BHK-21 and control cells after infected with influenza A virus，the method was also used to detect the dynamic changes of virus load in Cirp+BHK-21 cells which were blocked by the NF-κB inhibitor PDTC.The results of Western blot exhibited that overexpressed CIRP could significantly increase the expression of phosphorylation level of NF-κB (P<0.05)，but had no significant effect on the phosphorylation level of ERK.The results of quantitative detection of virus showed that overexpressed CIRP could significantly enhance the proliferation of influenza A virus，the virus load in the Cirp+BHK-21 cells were 111%，103%，167% and 235% (P<0.05) at 3，9，15 and 21 h PI，respectively，compared to the control group；Blocking the NF-κB was significantly decreased the virus load in the Cirp+BHK-21，and the virus load in treatment group were 98%，42%，19% (P<0.05)，7% (P<0.05) at 3，9，15 and 21 h PI，respectively，compared to the unblock group.Therefore，this study confirmed that overexpressed CIRP could enhance the proliferation of influenza A virus via activation of NF-κB pathway.

Key words: cold-inducible RNA binding protein, influenza A virus, replication, NF-κB, BHK-21 cells

CLC Number:

S852.23

NIE Pei-ting，TANG Cheng，YUE Hua. Activation of Cold-inducible RNA-binding Protein by H1N1 Influenza Virus Contributes to Viral Replication Via Activating NF-κB Pathway[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2108-2114.

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Activation of Cold-inducible RNA-binding Protein by H1N1 Influenza Virus Contributes to Viral Replication Via Activating NF-κB Pathway

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