公山羊Protamine 1 mRNA表达特性及与精液品质相关性的研究

doi:10.11843/j.issn.0366-6964.2016.07.007

Abstract

Abstract:

The aims of this study were to evaluate the expression profiles of Protamine 1（PRM1） mRNA in reproductive organs of bucks and to analyze the correlation between expression of PRM1 mRNA in spermatozoa and semen quality index.In this experiment，mRNA expression was detected by real-time fluorescent quantitative PCR.Cellular localization of PRM1 in testis was examined by immunohistochemistry.The correlation was analyzed by GraphPad Prism 5 software.The results showed that：the expression of PRM1 mRNA in testis was the highest in all tissue detected，which was 247 times higher than that in the epididymal caput（P＜0.05）.The expression of PRM1 mRNA in epididymal caput was significantly higher than that in epididymal corpus and cauda（P<0.05）.The expression of PRM1 mRNA in other tissues was very few.The expression of PRM1 mRNA in testis was gradually increased with the increase of ages in one year old.The PRM1 mRNA expression in testis at 12 months old were significantly higher than that at 9 months old（P＜0.05）.The PRM1 mRNA expression in testis at 9 months old was significantly higher than that in other ages.Concentration of PRM1 mRNA in sperm was positively correlated with the sperm motility （R2=0.586 0，P=0.000 5） and sperm concentration （R2=0.442 2，P=0.004 9）.PRM1 was located in elongating spermatids and spermatozoa in testis of bucks.There was no positive signal in other cells of testis.This study indicated that the expression of PRM1 mRNA was spatial and temporal-specific pattern.The sperm PRM1 mRNA content could be index of fertility of bucks.

Key words: Protamine 1, expression profiles, index of semen quality, correlation, bucks

CLC Number:

S827.2

ZHANG Chun-xiang，ZHAO Hui，GUO Li-na，ZHENG Ya-lin，ZHANG Cai-xia，LIU Wen-zhong，QIAO Li-ying，REN You-she. Expression Profiles of Protamine 1 mRNA and Correlation with Index of Semen Quality in Bucks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1354-1362.

References

［1］HECHT N B.Regulation of ‘haploid expressed genes’ in male germ cells［J］.J Reprod Fertil，1990，88(2)：679-693.
［2］OLIVA R，DIXON G H.Vertebrate protamine gene evolution I.Sequence alignments and gene structure［J］.J Mol Evol，1990，30(4)：333-346.
［3］DADOUNE J P.The nuclear status of human sperm cells［J］.Micron，1995，26(4)：323-345.
［4］STEGER K.Transcriptional and translational regulation of gene expression in haploid spermatids［J］.Anat Embryol，1999，199(6)：471-487.
［5］DEROUCHEY J E，RAU D C.Role of amino acid insertions on intermolecular forces between arginine peptide condensed DNA helices：implications for protamine-DNA packaging in sperm［J］. J Biol Chem，2011，286(49)：41985-41992.
［6］DOYEN C M，MOSHKIN Y M，CHALKLEY G E，et al.Subunits of the histone chaperone CAF1 also mediate assembly of protamine-based chromatin［J］.Cell Rep，2013，4(1)：59-65.
［7］AOKI V W，CARRELL D T.Human protamines and the developing spermatid：their structure，function，expression and relationship with male infertility［J］.Asian J Androl，2003，5(4)：315-324.
［8］STEGER K，FINK L，FAILING K，et al.Decreased protamine-1 transcript levels in testes from infertile men［J］.Mol Hum Reprod，2003，9(6)：331-336.
［9］ALEEM M，PADWAL V，CHOUDHARI J，et al.Sperm protamine levels as indicators of fertilising potential in sexually mature male rats［J］. Andrologia，2008，40(1)：29-37.
［10］AOKI V W，LIU L，CARRELL D T.Identification and evaluation of a novel sperm protamine abnormality in a population of infertile males［J］.Hum Reprod，2005，20(5)：1298-1306.
［11］POLLISTER A W，MIRSKY A E.The nucleoprotamine of trout sperm［J］.J Gen Physiol，1946，30(2)：101-116.
［12］BREWER L R，CORZETT M，LAU E Y，et al.Dynamics of protamine 1 binding to single DNA molecules［J］.J Biol Chem，2003，278(43)：42403-42408.
［13］CREE L H，BALHORN R，BREWER L R.Single molecule studies of DNA-protamine interactions［J］.Protein Pept Lett，2011，18(8)：802-810.
［14］BREWER L R，CORZETT M，BALHORN R.Protamine-induced condensation and decondensation of the same DNA molecule［J］. Science，1999，286(5437)：120-123.
［15］BALHORN R，BREWER L，CORZETT M.DNA condensation by protamine and arginine-rich peptides：analysis of toroid stability using single DNA molecules［J］.Mol Reprod Dev，2000，56(2 Suppl)：230-234.
［16］FORTES M R，SATAKE N，CORBET D H，et al.Sperm protamine deficiency correlates with sperm DNA damage in Bos indicus bulls［J］.Andrology，2014，2(3)：370-378.
［17］DOGAN S，VARGOVIC P，OLIVEIRA R，et al.Sperm protamine-status correlates to the fertility of breeding bulls［J］.Biol Reprod，2015，92(4)：92.
［18］GANGULY I，GAUR G K，KUMAR S，et al.Differential expression of protamine 1 and 2 genes in mature spermatozoa of normal and motility impaired semen producing crossbred Frieswal (HF× Sahiwal) bulls［J］.Res Vet Sci，2013，94(2)：256-262.
［19］COURTENS J L，PLÖEN L，LOIR M.Immunocytochemical localization of protamine in the boar testis［J］.J Reprod Fertil，1988，82(2)：635-643.
［20］RODRIGUEZ-MARTINEZ H，COURTENS J L，KVIST U，et al.Immunocytochemical localization of nuclear protamine in boar spermatozoa during epididymal transit［J］.J Reprod Fertil，1990，89(2)：591-595.
［21］GOSÁLVEZ J，LÓPEZ-FERNÁNDEZ C，FERNÁNDEZ J L，et al.Relationships between the dynamics of iatrogenic DNA damage and genomic design in mammalian spermatozoa from eleven species［J］.Mol Reprod Dev，2011，78(12)：951-961.
［22］李振，任有蛇，焦光月，等.雄激素受体基因在绵羊公羔不同组织中的表达特性及性腺中的定位［J］.畜牧兽医学报，2013，43(8)：1251-1257.
LI Z，REN Y S，JIAO G Y，et al.The expression of androgen receptor gene in different tissues and cellular localization in gonad of male sheep lambs［J］. Acta Veterinaria et Zootechnica Sinica，2013，43(8)：1251-1257.(in Chinese)
［23］任有蛇，张国林，郭丽娜，等.公山羊β防御素104a生物信息学分析及表达特性研究［J］.畜牧兽医学报，2015，46(2)：219-227.
REN Y S，ZHANG G L，GUO L N，et al.Bioinformatics analysis and expression characteristics of goat beta-defensin 104a gene in adult bucks［J］.Acta Veterinaria et Zootechnica Sinica，2015，46(2)：219-227.(in Chinese)
［24］任有蛇，秦小伟，郭丽娜，等.日粮纳米锌水平对公羊睾丸和附睾Cu-ZnSOD表达的影响［J］.畜牧兽医学报，2014，45(10)：1622-1630.
REN Y S，QIN X W，GUO L N，et al.Effect of the supplementation of different nano-zinc levels on expression of copper zinc superoxide in testis and epididymis of ram lambs［J］. Acta Veterinaria et Zootechnica Sinica，2014，45(10)：1622-1630.(in Chinese)
［25］张春香，秦小伟，郭丽娜，等.纳米锌水平对公羊精液品质、抗氧化酶活性及附睾Cu-ZnSOD表达的影响［J］.中国农业科学，2015，48(1)：154-164.
ZHANG C X，QIN X W，GUO L N，et al.Effect of different Nano-zinc levels in dietary on semen quality，activities of antioxidant enzyme and expression of copper zinc superoxide in epididymis of ram lambs［J］.Scientia Agricultura Sinica，2015，48(1)：154-164.(in Chinese)
［26］任有蛇，郭丽娜，张春香，等.山羊Lcn 5的表达特点及其在繁殖器官中定位［J］.畜牧兽医学报，2015，46(5)：711-718.
REN Y S，GUO L N，ZHANG C X，et al.Expression characteristics of Lcn 5 and its localization in reproduction organ of bucks［J］.Acta Veterinaria et Zootechnica Sinica，2015，46(5)：711-718.(in Chinese)
［27］DOMENJOUD L，KREMLING H，BURFEIND P，et al.On the expression of protamine genes in the testis of man and other mammals［J］.Andrologia，1991，23(5)：333-337.
［28］FEI R R，JI L，WU X F，et al.Studies on expression of P1 protamine gene in rat and mouse testis［J］.Reproduction & Contraception，1999，10(4)：195-202.
［29］滕小梅，韩泽广，黄健.PRM1，一个新的候选结肠癌相关CT抗原基因的识别及其在结肠癌组织中的表达分析［J］.现代检验医学杂志，2008，23(3)：1-4.
TENG X M，HAN Z G，HUANG J.Identification and expression analysis of PRM1 as a novel candidate cancer/testis gene in human colorectal cancer［J］.Journal of Modern Laboratory Medicine，2008，23(3)：1-4.(in Chinese)
［30］侯文佳，陈芳芳，任平，等.鱼精蛋白 1 在结肠腺癌组织中的表达及其临床意义［J］.中国生物制品学杂志，2013，26(5)：698-700，709.
HOU W J，CHEN F F，REN P，et al.Expression and significance of protamine 1 in human colon adenocarcinoma tissue［J］.Chinese Journal of Biologicals，2013，26(5)：698-700，709.(in Chinese)
［31］NILI H A，MOZDARANI H，ALEYASIN A.Correlation of sperm DNA damage with protamine deficiency in Iranian subfertile men［J］.Reprod Biomed Online，2009，18(4)：479-485.
［32］STEGER K，WILHELM J，KONRAD L，et al.Both protamine-1 to protamine-2 mRNA ratio and Bcl2 mRNA content in testicular spermatids and ejaculated spermatozoa discriminate between fertile and infertile men［J］.Hum Reprod，2008，23(1)：11-16.
［33］PARADOWSKA-DOGAN A，FERNANDEZ A，BERGMANN M，et al.Protamine mRNA ratio in stallion spermatozoa correlates with mare fecundity［J］. Andrology，2014，2(4)：521-530.
［34］LÜKE L，CAMPBELL P，VAREA SÁNCHEZ M，et al.Sexual selection on protamine and transition nuclear protein expression in mouse species［J］.Proc Biol Sci，2014，281(1783)：76-80.
［35］RETTIE E C，DORUS S.Drosophila sperm proteome evolution：Insights from comparative genomic approaches［J］.Spermatogenesis，2012，2(3)：213-223.
［36］MEISTRICH M L，TROSTLE-WEIGE P K，VAN BEEK M E.Separation of specific stages of spermatids from vitamin A-synchronized rat testes for assessment of nucleoprotein changes during spermiogenesis［J］.Biol Reprod，1994，51(2)：334-344.
［37］LESCOAT D，BLANCHARD Y，LAVAULT M T，et al.Ultrastructural and immunocytochemical study of P1 protamine localization in human testis［J］.Andrologia，1993，25(2)：93-99.
［38］LE LANNIC G，ARKHIS A，VENDRELY E，et al.Production，characterization，and immunocytochemical applications of monoclonal antibodies to human sperm protamines［J］.Mol Reprod Dev，1993，36(1)：106-112.
［39］费仁仁，邓锦松，纪林，等.大鼠精子细胞变态期核蛋白转换的研究［J］.解剖学报，1997，28(4)：388-392.
FEI R R，DENG J S，JI L，et al.Studies on rat spermatid nuclear protein transition［J］.Acta Anatomica Sinica，1997，28(4)：388-392.(in Chinese)

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Expression Profiles of Protamine 1 mRNA and Correlation with Index of Semen Quality in Bucks

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