绵羊季节性繁殖相关基因TSHR外显子多态性研究

doi:10.11843/j.issn.0366-6964.2016.07.006

Abstract

Abstract:

The study aimed to investigate the genetic variation of thyroid stimulating hormone receptor (TSHR) gene in Ujumqin sheep and Hu sheep，which had different reproductive performance and oestrous cycle，and the distribution of different haplotypes caused by the polymorphic site and its effects on mRNA and secondary structure of protein.This study provided a theoretical basis for seeking the influence of the mutation on TSHR gene expression and phenotype.Direct sequencing was taken to study genetic variation of the TSHR gene in 172 sheep of 2 Chinese Mongolian sheep strains.6 mutant sites were found and genotyped.Chi-square test for independence was performed to find the genotypes of the 4 SNPs (SNP1，SNP3，SNP4 and SNP5) which were significantly different between the 2 sheep populations（P＜0.01）.The SNP5 was missense mutation，the other sites were synonymous mutations.Linkage disequilibrium analysis was used to screen the 4 sites，complete linkage happened between SNP3 and SNP4，and the other sites present different degrees of interlocking relationship.Bioinformatics analysis revealed that not only the mutations occured in the TSHR，but also different haplotypes could result in the changes of mRNA and the secondary structure of protein.These results indicate that：(1) The genotypes distribution of 4 sites in TSHR gene have significant differences between the 2 sheep populations.They form 6 haplotypes，among which H1(CGCC) is the advantage haplotype in Ujumqin sheep，and H4(TTTG) is the advantage haplotype in Hu sheep.(2) The SNP5 site affects the stability of mRNA and protein secondary structure of TSHR gene，which may be one of the important functional sites with potential phenotypic effects.

Key words: sheep, seasonal breeding, TSHR gene, polymorphism

CLC Number:

S826
S813.3

XUAN Jun-li，MA Xiao-meng，WANG Hui-hua，CAO Jia-xue，WEI Cai-hong，ZHAO Fu-ping，MA You-ji，ZHANG Li，DU Li-xin. Study on Exon Polymorphism of Seasonal Breeding Related Gene TSHR in Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1342-1353.

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Study on Exon Polymorphism of Seasonal Breeding Related Gene TSHR in Sheep

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