谷胱甘肽促进牛体外受精胚胎发育的转录组初探

doi:10.11843/j.issn.0366-6964.2016.07.008

Abstract

Abstract:

To elucidate the effects of exogenous glutathione (GSH) on embryos transcriptome，bovine IVF embryos at 8-16-cell and morula stage were collected for high-throughput RNA sequencing (RNA-seq).Quantitative determination of genes expression was used to verify the RNA-seq data.Gene ontology and KEGG pathway enrichment analysis of differential expression genes were performed.The accuracy and validity of RNA-seq data were confirmed with combination of error rate of base，mapping of reads to bovine genome，distribution region analysis and consistency between qPCR and RNA-seq results of ten genes.A total of 4 100 genes were differentially expressed between embryos at two stages and 3 952 genes were identified as expressed in embryos treated with GSH.A high enrichment of genes involved in 84 GO terms，such as ATP synthesis，respiratory chain，DNA synthesis and translation.Pathway analysis revealed 27 pathways involved in cell adhesion，TCA cycle，GSH metabolism and amino acid metabolism.Additionally，2 known conserved domains (S-TKc，MH1) and 2 new domains (LCR，DWB) were found in 5 new transcripts by prediction，compared with known transcripts.To sum up，GSH treatment can result into the comprehensive change at transcriptional level of bovine IVF embryos and improve their development consequently.

Key words: bovine IVF embryo, RNA-Seq, qRT-PCR, novel isoform, GO analysis, KEGG pathway analysis

CLC Number:

S823

YU Xue-ying，GUO Qin-qin，HAO Hai-sheng，SUN Wei-jun，ZHAO Xue-ming，ZHU Hua-bin，YANG Ling，DU Wei-hua. Transcriptome of Bovine IVF Embryos Treated with Glutathione[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1363-1372.

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Transcriptome of Bovine IVF Embryos Treated with Glutathione

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