猪miR-135a-5p预测靶基因APC的鉴定

doi:10.11843/j.issn.0366-6964.2015.04.004

Abstract

Abstract:

This research aimed to investigate the potential target genes related to adipose tissue development.The bioinformatics softwares were used to predict the target gene of ssc-miR-135a-5p，and it was found that the 3′ UTR of adenomatous polyposis coli (APC) gene had 2 putative binding sites with miR-135a-5p.The wild-type (APC 3′ UTR) and 3 mutated (APC 3′ UTR m1，APC 3′ UTR m2 and APC 3′ UTR m3) dual-luciferase vectors were constructed and then co-transfected into PK-15 cells with miR-135a-5p mimics or negative control (NC)，respectively，and then the luciferase activity was detected.The results showed that miR-135a-5p mimics significantly decreased the luciferase activities of APC 3′ UTR and APC 3′ UTR m2 vectors (P<0.05)，while APC 3′ UTR m1 and APC 3′ UTR m3 completely abolished the inhibitory effects of miR-135a-5p，which demonstrated that miR-135a-5p mainly binded with the first site in 3′ UTR of APC gene.Further analysis revealed that miR-135a-5p inhibited the levels of endogenous APC mRNA and protein in porcine preadipocytes，which indicate that the target gene of ssc-miR-135a-5p is APC gene and regulates its expression.Taken together，this study will provide new insight into the biological function of ssc-miR-135a-5p and its mechanism in regulating adipogenesis.

Key words: pigs, miR-135a-5p, APC, target

CLC Number:

S828
S813.3

CHEN Chen，DENG Yuan，REN Hui-bo，ZHU Ji，HU Xiong-gui，JIANG Si-wen，PENG Ying-lin. Identification of APC as the Potential Target of miR-135a-5p in Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 534-541.

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Identification of APC as the Potential Target of miR-135a-5p in Pigs

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