褪黑激素对内蒙古绒山羊皮肤中let-7家族及其靶基因的影响

doi:10.11843/j.issn.0366-6964.2019.07.006

Abstract

Abstract: The study aimed to explore the effects of melatonin on the periodic expression of let-7 family and its target genes in skin of Inner Mongolia Cashmere goat. In this study, six Inner Mongolia Hanshan Cashmere goats were selected and divided equally and randomly into 2 groups, melatonin implantation group and non-implantation group. Taking the skin tissue of the goats as experimental material in every months of the year. The expression changes of 11 members in let-7 family were quantified by reverse transcription-real time polymerase chain reaction (qRT-PCR) in the skin tissue. The expression changes of the target genes of let-7 family were analyzed using the RNA-Seq technique. The results showed that:1) During the whole hair follicle cycle, melatonin up-regulated the expression of let-7b, let-7e and let-7a-3p, down-regulated the expression of let-7f, let-7g, let-7c, let-7a-5p, let-7d and miR-98. 2) In April, melatonin up-regulated the expression of "let-7g, let-7i and miR-98", down-regulated the expression of target genes such as PTGS2 and PLCG1. It down-regulated the expression of "let-7a-5p, let-7b and let-7c", up-regulated the expression of target genes such as TGIF2 and RBL1. 3) In June, melatonin down-regulated the expression of "let-7g, let-7i and miR-98", up-regulated the expression of target genes such as PTGS2, PLCG1 and SRC; It up-regulated the expression of "let-7a-5p, let-7b and let-7c", down-regulated the expression of target genes such as GDF5, TGIF2 and RBL1. 4) The target genes of let-7 family were mainly involved in VEGF, TGF-β, Oxytocin and NF-kappa B signaling pathways, and played an important role in cell processes, hormone regulation and regulation of transcription factors. In summary, MT may affect the expression of the corresponding target genes by changing the expression level of members in the let-7 family. It plays an important role in the periodic growth of skin hair follicles. These results provided a theoretical basis for further revealing the molecular mechanism of melatonin mediating miRNAs to affect cashmere growth.

CLC Number:

S826.2

WANG Jianmeng, FU Shaoyin, LI Chun, BAI Xue, LIU Bin, JI Xiaoyang, LIU Yongbin, ZHANG Wenguang. Effects of Melatonin on let-7 Family and Its Target Genes in Skin of Inner Mongolian Cashmere Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1367-1379.

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Effects of Melatonin on let-7 Family and Its Target Genes in Skin of Inner Mongolian Cashmere Goats

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