绵羊AA-NAT基因mRNA表达与常年发情相关性研究

doi:10.11843/j.issn.0366-6964.2015.04.005

Abstract

Abstract:

In order to study the association of AA-NAT gene and year-round estrus in sheep，year-round estrous Small Tail Han ewes and seasonal estrous Tan ewes were selected as tested subjects.Real-time PCR method was used to detect AA-NAT mRNA expression in hypothalamus，pituitary gland，pineal gland，ovary，uterus body and adrenal gland at different estrus stages.The results showed that the expression of AA-NAT gene in all 6 tissues at different estrous stages of the 2 breeds was significantly different.The expression of AA-NAT gene was at high level in ovary，uterus body and pineal gland in sheep at estrus.In ovary and uterus body， AA-NAT gene expression was significantly lower (P<0.01) in Small Tail Han sheep at spring and fall estrus stage than that in Tan sheep at fall estrus stage，but in pineal gland it was significantly higher (P<0.01).The results preminarily indicated that the up-regulated expression of AA-NAT gene in ovary，uterus body and pineal gland was associated with year-round estrus in Small Tail Han sheep.

Key words: sheep, year-round estrus, AA-NAT gene, real-time PCR, gene expression

CLC Number:

S826
S814.8

LU Lu-lu，SUN Xiao-di，CHU Ming-xing，WANG Yong-juan，HUANG Dong-wei，WANG Jin-yu，DI Ran，PAN Zhang-yuan，LIU Qiu-yue. Association between AA-NAT mRNA Expression and Year-round Estrus in Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 542-548.

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Association between AA-NAT mRNA Expression and Year-round Estrus in Sheep

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