猪T细胞受体β链的基因结构及其多样性分析

doi:10.11843/j.issn.0366-6964.2014.11.019

Abstract

Abstract:

Based on the gene of porcine T cell receptor β chain (TCRβ) from the GenBank database，82 of swine T cell receptor β chain genes (STB) were cloned by reverse transcription-polymerase chain reaction (RT-PCR) from porcine lymphocyte of peripheral-blood，lymphoid node and spleen with one pair primers.Sequence analysis showed that STB genes were all contain a domain of variable signal peptide and V，hypervariable D and J, and conservative C domain.However，nucleotide sequence of STB was not complete identical with 36.6%-99.9% homology among genes，and had extremely sophisticated polymorphism and diversity.This was concord to the genetic structure of TCRβ chain.Molecular structure，genetic evolution and classify of porcine TCRβ genes displayed that mutations on the domain of signal peptide，FR1 and CDR1，FR2 and CDR2，as well as FR3 were caused by polymorphism of the STBV.And differences of CDR3 domain，however，were caused by diversity of the STBD and STBJ.Analysis of similarity and classify of TCRβV domain (STBV)，D domain (STBD) and J domain (STBJ) of swine compared with human，found the genetic evolution relationship was closer，and each of TRBV，STBD and TRBJ also had a corresponding fragment of human.This indicated that pig possessed the genetic diversity of immunology against complicated environment of microbes in healthy status.

Key words: Hezuo minipig, gene of TCR&beta, chain, bioinformatics, diversity

CLC Number:

S852.4

ZENG Shuang，LI Wan-sheng，FANG Yong-xiang，FENG Hai-yan，CHEN Guo-hua，HE Xiao-bing，JIA Huai-jie，JING Zhi-zhong. Genetic Structure and Diversity of Porcine T Cell Receptor β Chain Genes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1874-1882.

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Genetic Structure and Diversity of Porcine T Cell Receptor β Chain Genes

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