ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (5): 1012-1019.doi: 10.11843/j.issn.0366-6964.2018.05.016

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Genetic Diversity of Psoroptes ovis var. cuniculi in China, Basing on the Complete Sequence of Mitochondrial 16S rRNA

GU Jiang, LI Yan-fang, ZHAO Xi-bin, XIE Yue, LAI Wei-min, YANG Guang-you, GU Xiao-bin*   

  1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2017-10-18 Online:2018-05-23 Published:2018-05-23

Abstract:

In order to evaluate the genetic variation and population structure of Psoroptes ovis var. cuniculi, the full-length of the mitochondrial 16S rRNA of 83 isolates of Psoroptes ovis var. cuniculi from 5 geographic regions in China (including North, East, central, southwest, northwest zones) were amplified using the PCR technology, sequenced and then genetically analyzed. The results showed that the full 16S rRNA gene from 83 Psoroptes ovis var.cuniculi isolates were 1 025 bp in length, and that there were a total of 83 polymorphic sites and 52 haplotypes; the values of Fst and the gene flow between 5 geographical populations were -0.037 59-0.587 63 and -6.900 7-31.117 62, respectively; NJ tree and haplotype network analysis further showed these 52 haplotypes formed two branches with a messy distribution, and no population cluster patterns according to the geographical area, temperature zone and rabbit breeds were observed. These results indicate that Psoroptes ovis var.cuniculi population in China appeared a high genetic diversity and differentiation, but the geographical population structure has not formed.

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