免疫抑制性病毒感染肉鸡群继发大肠杆菌的分离鉴定及多样性研究

doi:10.11843/j.issn.0366-6964.2014.11.017

Abstract

Abstract:

This study aimed to investigate the diversity of serotype and drug resistance of E.coli from the secondary infection of broilers that primarily infected by immunosuppressive viruses.E.coli isolates derived from one-day-old broilers single-infected or co-infected with avian leukosis virus subgroup J (ALV-J) and reticuloendotheliosis virus (REV) were isolated，identified and used for drugs sensitivity experiment.Five red or (and) white clones were picked from the diseased organs.Results showed that 516 E.coli isolates were isolated from 58 diseased chickens and identified from two repeated experiments including 445 red colonies and 71 white colonies.Results also reveal that the co-infection could infect more serious mortality and among these 516 tested E.coli colonies，12 serotypes of O-antigen were identified.E.coli of different serotypes was even isolated from the same organ in the same bird.Then 17 groups of colonies (5 same O-serotype colonies isolated from the same organ in the same bird were identified as a group of colonies) were selected to drug susceptibility test for 12 kinds of common antibiotics.The results showed that the minimum inhibitory concentration was from 0.05 to 51.20 mg•mL^－1 in the third group to colimycin and from 0.05 to 51.20 mg•mL^－1 in the seventeen group to amikacin.However，differences in the other groups were not significant.The high-degree diversity of E.coil isolated from our experiments proved that the infection was the secondary E.coil infection.It was happened in the immunosupression condition under the viruses infection.At the same time，the diversity of drug resistance is the genetic basis for resistant strains rapidly generated from the E.coil in vivo under the condition of irrational use of antibiotics.

Key words: immunosuppressive viruses, broilers, E.coli, serotype, drug resistance

CLC Number:

S852.612

JU Si-di，DONG Xuan，ZHAO Peng，LI Yang，MENG Fan-feng，SUN Peng，DONG Xiang-lei，GAO Song，CUI Zhi-zhong. Isolation，Identification and Diversity Study of Escherichia coli from Broilers Infected with Immunosuppressive Viruses[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1858-1865.

References

［1］CALNEK B W.禽病学［M］.高福，刘文军，译.9版.北京:北京农业大学出版社,1991：126-133.
［2］DHO-MOULIN M，FAIRBROTHER J M.Avian pathogenic Escherichia coli (APEC) ［J］.Vet Res，1999，30(2-3)：299-316.
［3］BLANCO J E，BLANCO M，MORA A，et al.Serotypes of Escherichia coli isolated from septicaemic chickens in Galicia (Northwest Spain) ［J］.Vet Microbiol，1998，31:229-235.
［4］陆承平.兽医微生物学［M］.3版.北京:中国农业出版社，2001：215-223.
［5］张春荣，苏亚拉图.我国流行的鸡致病性大肠杆菌血清型［J］.中国动物检疫，1996，13(1)：27-27.
［6］崔治中.鸡群中免疫抑制性病毒的共感染及其相互作用——对新城疫和禽流感疫苗免疫反应的抑制作用［J］.中国动物检疫，2007，24(10)：45-48.
［7］YANG H，CHEN S，WHITE D G，et al.Characterization of multiple-antimicrobial-resistant Escherichia coli isolates from diseased chickens and swine in China［J］.J Clin Microbiol，2004，42(8)：3483-3489.
［8］NEU H C.The crisis in antibiotic resistance［J］.Science，1992，257(5073)：1064-1073.
［9］WITTE W.Medical consequences of antibiotic use in agriculture［J］. Science，1998，279(5353)：996-997.
［10］ALEKSHUN M N，LEVY S B.Molecular mechanisms of antibacterial multidrug resistance［J］.Cell，2007，128(6)：1037-1050.
［11］SUNDE M，NORSTRÖM M.The prevalence of，associations between and conjugal transfer of antibiotic resistance genes in Escherichia coli isolated from Norwegian meat and meat products［J］. J Antimicrob Chemother，2006，58(4)：741-747.
［12］丁元廷.细菌耐药机制的国内外最新研究进展［J］.现代预防医学，2013，40(6)：1109-1111.
［13］吕晓菊.细菌耐药的机制与对策［J］.临床内科杂志，2007，24(5)：295-297.
［14］CUI Z，DU Y，ZHANG Z，et al.Comparison of Chinese field strains of avian leukosis subgroup J viruses with prototype strain HPRS-103 and United States strains［J］.Avian Dis，2003，47(4)：1321-1330.
［15］WANG Y，CUI Z Z，JIANG S.Sequence analysis for the complete proviral genome of reticuloendotheliosis virus Chinese strain HA9901［J］.Sci China Ser C-Life Sci，2006，49(2)：149-157.
［16］成大荣，朱善元，钱金梅，等.不发酵或迟发酵乳糖型禽大肠埃希菌的分离鉴定［J］.动物医学进展，2009，30(5)：28-31.
［17］CHEN X，GAO S，JIAO X A，et al.Prevalence of serogroups and virulence factors of Escherichia coli strains isolated from pigs with postweaning diarrhoea in eastern China［J］.Vet Microbiol，2004，103(1-2)：13-20.
［18］陈祥，高崧，王雷，等.华东地区致初生仔猪腹泻大肠杆菌的 O 血清型和毒力因子［J］.微生物学报，2004，44(1)：96-100.
［19］JIANG H，LV D，CHEN Z，et al.High prevalence and widespread distribution of multi-resistant Escherichia coli isolates in pigs and poultry in China［J］.Vet J，2011，187(1)：99-103.
［20］WATTS J L.Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals：approved standard：NCCLS documents M31-A［S］.NCCLS，1999.
［21］宋立，宁宜宝，张秀英，等.中国不同地区家禽大肠杆菌血清型分布和耐药性比较研究［J］.中国农业科学，2005，38(7)：1466-1473.
［22］高璐，胡仁莉，陈祥，等.SPF鸡人工感染禽源大肠杆菌、H9亚型禽流感病毒后的体液免疫应答和死亡率分析［J］.畜牧兽医学报，2006，37(9)：899-902.

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Isolation，Identification and Diversity Study of Escherichia coli from Broilers Infected with Immunosuppressive Viruses

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