禽戊型肝炎病毒组织切片免疫荧光检测方法的建立及应用

doi:10.11843/j.issn.0366-6964.2014.11.016

Abstract

Abstract:

Immunofluorescence staining method on paraffin-embedded liver sections was established to differentially detect avian HEV in this experiment.Mono-specific serum of HEV ORF2 protein were prepared and used for detecting tissue section of lesion liver of chickens infected with HEV，avian leukosis virus(ALV)，reticuloendotheliosis virus (REV)，respectively.Results showed that the prepared mono-specific serum of HEV ORF2 protein showed good specificity.The established immunofluorescence method could detect avian HEV infection and was effective to distinguish HEV from ALV or REV infection.Our results revealed that the novel immunofluorescence method brings great convenience for laboratory diagnosis.

Key words: avian hepatitis E virus, tissue section, immunofluorescence, differential diagnosis

CLC Number:

CHEN Rui-ai，LI Yan-peng，TONG Ge，GUO Kai，LIU Zheng-wei. Establishment and Application of Tissue Section Immunofluorescence Method for Detecting Avian Hepatitis E Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1851-1857.

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Establishment and Application of Tissue Section Immunofluorescence Method for Detecting Avian Hepatitis E Virus

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