捻转血矛线虫阿苯达唑耐药株给药前后比较转录组学分析

doi:10.11843/j.issn.0366-6964.2019.03.019

Abstract

Abstract:

Aiming to find the transcription difference of albendazole resistant strain of Haemonchus contortus,before and after administration. In this study, Samples of bBZ (before albendazole administration) and aBZ (after albendazole administration) were analyzed using Illumina Hiseq4000 RNA sequencing platform and their transcriptome libraries were constructed. Screening for differentially expressed genes(DEGs) and functional annotation and enrichment analysis by GO and KEGG databases,at the same time,they were verified by real-time PCR. The results showed that:a total of 851 DEGs between aBZ and bBZ, of which 584 and 267 genes were up-and down-regulated, respectively. The DEGs were then searched against the GO and KEGG database for enrichment analysis. GO analysis showed that 458,418 and 367 DEGs were annotated into biological process, cellular component and molecular function. KEGG analysis showed that 173 differentially expressed genes were assigned to 75 KEGG pathways, and clustered significantly in ribosome synthesis, apoptosis-multiple species, PPAR signaling pathway, and so on. This experiment initially screened DEGs between bBZ and aBZ of Haemonchus contortus. The study provides a foundation for exploring the molecular mechanism of resistance to Haemonchus contortus, screening for molecular markers of drug resistance detection and the establishment of early differential diagnosis methods for drug resistance.

CLC Number:

S855.9

ZHAO Xueliang, WANG Shuyi, HU Hebateer, SUN Ke, SU Qian, Lü Xu, WANG Wenlong, LIU Chunxia. Comparative Transcriptome Analysis between before and after Administration of Albendazole Resistant Strain of Haemonchus contortus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 637-644.

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Comparative Transcriptome Analysis between before and after Administration of Albendazole Resistant Strain of Haemonchus contortus

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