ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (1): 33-38.

• 遗传繁育 • Previous Articles     Next Articles

Cloning, Sequence Analysis and Specific Expression in Different Tissues of Duck Akirin2 Gene

DAI Fei, HUANG Kai-liang, LIU He-he, HAN Chun-chun, LI Liang, WANG Ji-wen*   

  1. Key Lab of Animal Genetic Resources Exploitation and Utilization, Sichuan Agricultural University, Ya′an 625014, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-25 Published:2011-01-25

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Abstract: In order to understand the function of Akirin2 gene in duck, the duck Akirin2 gene was cloned by RT-PCR, and the relative mRNA expression of Akirin2 was detected in different tissues using the qPCR method. The results of the sequence analysis showed that the CDS region of the duck Akirin2 gene included 594 nucleotides, encoding for 197 amino acids, and the amino acid homology of Akirin2 between the duck and the chicken was 99%. The secondary structure of Akirin2 amino acids had a common domain with the mammals Akirin1 and Akirin2.The results of the qRT-PCR indicated that the duck Akirin2 express in the muscle and the immune organ spleen. These results supported the previously views that duck Akirin2 gene had a common function with the Akirin1 and Akirin2 genes in mammals, and these assays made a foundation work for the further research about the Akirin2 gene in myogenesis in birds.

Key words: duck, Akirin2, sequence analysis, qPCR