ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (1): 1-7.

• 遗传繁育 •     Next Articles

Influence of Castration on Porcine Thyroid Transcription Factor-1,2 (TTF-1, 2)Gene Expression

WANG Ying， ZHANG Li-fan， CAI Zhao-wei， CHEN Zhe， JIANG Xiao-ling， ZHOU Hong-mei，HUA Xu-chuan， XU Ning-ying*   

  1. College of Animal Science, Zhejiang University, Hangzhou 310029, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-25 Published:2011-01-25

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Abstract: In order to investigate the effect of castration on expression of TTF-1 and TTF-2 genes, realtime fluorescence quantitative PCR was applied to investigate the developmental expression patterns of TTF-1 and TTF-2 genes from the thyroid tissue of castrated and uncastrated Jinhua pigs on the age of 60, 90 and 120 days. The results showed that the expression of TTF-1 and TTF-2 genes increased slightly in uncastrated pigs, while they were relatively stable in castrated pigs. The expression level of TTF-1 and TTF-2 mRNA in castrated Jinhua pigs on 60 and 90 days were higher than that of uncastrated pigs, while the expression level of TTF-1 and TTF-2 on 120 days were lower than that of uncastrated pigs, but no significant difference （P>0.05). The results indicated that the real-time PCR method was faithful for detecting the expression analysis of TTF-1 and TTF-2 genes, and the expression of TTF-1 and TTF-2 mRNA increased after castration. Furthermore, there was a significant correlation between TTF-1 gene expression and meat quality traits.

Key words: pig, castration, real-time PCR, thyroid transcription factor 1 (TTF-1), thyroid transcription factor 2 (TTF-2)