Abstract:

To understand the effect of overfeeding on LXRα mRNA level, the Landes goose and Sichuan White goose were used to clone the partial gene sequence of Liver X Receptor α (LXRα) by RTPCR, and the effect of overfeeding on the transcriptional level of LXRα in liver and adipose tissues was researched by SYBRGreen
in this study. The result indicated that the obtained LXRα gene sequence was 1 005 bp, and had high similarity with other species. There were 8 amino acid variation sites. The mRNA expression of LXRα gene was detected in liver, abdominal adipose and subcutaneous adipose tissues of two breeds, while its expression level was the highest in liver. Overfeeding significantly increased the mRNA expression of LXRα gene in the three detected tissues. In control group, the mRNA level of LXRα in liver of Landes goose was significantly higher than that of Sichuan White goose, but the mRNA level of LXRα in abdominal adipose and subcutaneous adipose tissues of Landes goose was significantly lower than that of Sichuan White goose. In overfeeding group, the mRNA level of LXRα in liver and abdominal adipose tissues of Sichuan White goose was significantly higher than that of Landes goose, but there was no significant difference at the mRNA level of subcutaneous adipose tissues between the two breeds. The mRNA abundance of LXRα was positively correlated to the relative weight of liver, abdominal adipose and subcutaneous adipose tissues after overfeeding, and the correlation in Landes goose was stronger than that in Sichuan White goose. It was concluded that overfeeding induced the significant increase of LXRα mRNA level in goose liver and adipose tissues, and the effect of overfeeding was difference between the two breeds.

Key words: goose, overfeeding, LXRα, gene clone, mRNA expression