家兔小肠不同区段营养物质转运载体相关基因的分布规律

doi:10.11843/j.issn.0366-6964.2019.07.022

Abstract

Abstract: The objective of this study was to investigate the distribution of nutrient transporter related genes in different segments of small intestine of rabbit. Ten healthy white rex rabbits at 110 days old with similar weight were chose to slaughter. The intestinal segment samples were collected from duodenum, jejunum, ileum for detecting the mRNA abundance of oligopeptide transporter Pep Tl, amino acid transporter CAT1, B⁰AT, EAAT3, rBAT, glucose transporter SGLT1, GLUT2, GLUT5 and fatty acid transporter FATP4 by real-time PCR. The results showed that the abundance of oligopeptide transporter Pep T1 mRNA was the highest in duodenum, and lower in jejunum. The abundance of basic amino acid transporter CAT1, facultative amino acid transporter rBAT, and neutral amino acid transporter B⁰AT mRNA were the highest in ileum, and lower in jejunum. The acidic amino acid transporter EAAT3 mRNA expression was higher in jejunum and ileum. The expression of glucose transporter SGLT1 and GLUT5 mRNA was higher in duodenum and jejunum. The abundance of glucose transporter GLUT2 and fatty acid transporter FATP4 mRNA were the highest in jejunum, and lower in duodenum. The result indicate that the main part in intestine for transporting and absorbing oligopeptide, glucose and fatty acid is the first half of the small intestine, the main part for transporting and absorbing amino acid is the posterior segment of the small intestine.

CLC Number:

S829.1

CHEN Saijuan, LIU Yajuan, YUAN Wanzhe, LI Jiangtao, PANG Lixin, GU Zilin, CHEN Baojiang. Distribution of Nutrient Transporter Related Genes in Different Segments of Small Intestine of Rabbits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1518-1524.

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Distribution of Nutrient Transporter Related Genes in Different Segments of Small Intestine of Rabbits

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