水牛卵丘颗粒细胞凋亡与卵母细胞体外发育潜能的研究

ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (9): 1400-1404.

• 研究简报 • Previous Articles Next Articles

Study on the Relationship between Cumulus Granulose Cells Apoptosis and Oocyte Development in Buffalo

WANG Xiaoli, YANG Juan, CHEN Yu, CHENG Junping, PAN Hongping , WEI Jingwei, LU Fenghua , SHI Deshun*

College of Animal Science and Technology, Guangxi University, Nanning 530005, China

Received:2008-06-19 Revised:1900-01-01 Online:2009-09-24 Published:2009-09-24
Contact: SHI Deshun

Abstract

Abstract:

The relationship between the developmental ability of buffalo oocytes and the apoptosis of granulose cells was investigated in this study. Granulose cells were analyzed on the apoptosis by Annexin VFITC after the buffalo oocytes were cultured 2224 h in vitro, and which was compared with the maturity and the scoring of oocyte as well as the rate of cleavage and embryos. Results showed that the rate of apoptosis increased with the maturity of oocyte. While with the scoring and developmental capacity of the oocyte decreasing, the rate of apoptosis of granulose cells was increased. The results indicated that the relationship between the developmental capacity of the oocyte and apoptosis of granulose cells was significant. The apoptosis rate of granulose cells could be a developmental potential predictor of the immature oocytes.

Key words: cumulus granulosa cells, apoptosis, oocyte, IVM

WANG Xiaoli;YANG Juan;CHEN Yu;CHENG Junping;PAN Hongping;WEI Jingwei;LU Fenghua;SHI Deshun. Study on the Relationship between Cumulus Granulose Cells Apoptosis and Oocyte Development in Buffalo[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(9): 1400-1404.

[1]	XU Tao, PAN Yangyang, HE Honghong, LI Guyue, ZHANG Huizhu, ZHAO Ling, CUI Yan, YU Sijiu. The Effects of Tumor Necrosis Factor-α (TNF-α) on the Expression of HIF-1α and HSP70 in Yak Oocytes and the Subsequent Embryo Development [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1198-1207.
[2]	XU Tao, PAN Yangyang, HE Honghong, LI Guyue, ZHANG Huizhu, ZHAO Ling, CUI Yan, YU Sijiu. The Effects of Tumor Necrosis Factor-α (TNF-α) on the Expression of HIF-1α and HSP70 in Yak Oocytes and the Subsequent Embryo Development [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1198-1207.
[3]	YUAN Hongxia, LUO Jinhong, FENG Wenwu, CHEN Xiang. Study on the Effect of LYRM1 Gene on the Fat Deposition of Pig [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 677-687.
[4]	WANG Yong, MAO Ruoqing, ZHANG Keshan, ZHENG Haixue, LIU Xiangtao. The Structural Protein of Senecavirus A VP1 Induces Apoptosis in PK-15 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 811-820.
[5]	YUAN Hongxia, LUO Jinhong, FENG Wenwu, CHEN Xiang. Study on the Effect of LYRM1 Gene on the Fat Deposition of Pig [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 677-687.
[6]	WANG Yong, MAO Ruoqing, ZHANG Keshan, ZHENG Haixue, LIU Xiangtao. The Structural Protein of Senecavirus A VP1 Induces Apoptosis in PK-15 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 811-820.
[7]	XU Da, WANG Yang, XUAN Biao, WANG Qiuyue, XU Man, CHEN Xuan, JIN Yi. Inhibiton of Ubiquitin-conjugating Enzyme Alters the Ubiquitination Status of Porcine Oocyte Zona Pellucidas and Sperm-oocyte Binding Ability [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 544-550.
[8]	FANG Shu, ZHANG Jiamei, YANG Yi, HAN Lu, MA Chenjie, WU Xiaoling, DENG Guangcun. Regulation of Receptor-interacting Protein 1 on Apoptosis of Macrophage Induced by Bacillus Calmette-Guerin [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 645-653.
[9]	XU Da, WANG Yang, XUAN Biao, WANG Qiuyue, XU Man, CHEN Xuan, JIN Yi. Inhibiton of Ubiquitin-conjugating Enzyme Alters the Ubiquitination Status of Porcine Oocyte Zona Pellucidas and Sperm-oocyte Binding Ability [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 544-550.
[10]	FANG Shu, ZHANG Jiamei, YANG Yi, HAN Lu, MA Chenjie, WU Xiaoling, DENG Guangcun. Regulation of Receptor-interacting Protein 1 on Apoptosis of Macrophage Induced by Bacillus Calmette-Guerin [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 645-653.
[11]	GUO Zhenwei, HUANG Yongjun, FAN Weihong, WEN Dongmei, SHEN Penglei, PAN Erke, LU Fenghua, SHI Deshun. The Effect of Melatonin on the Maturation of Oocyte in vitro and Its Receptor-mediated Mechanism in Buffalo [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 314-322.
[12]	GUO Zhenwei, HUANG Yongjun, FAN Weihong, WEN Dongmei, SHEN Penglei, PAN Erke, LU Fenghua, SHI Deshun. The Effect of Melatonin on the Maturation of Oocyte in vitro and Its Receptor-mediated Mechanism in Buffalo [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 314-322.
[13]	HE Xiaoyun, LIU Qiuyue, CHU Mingxing. Advances in miRNA Regulating Mammalian Follicular Development and Oocyte Maturation [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2175-2185.
[14]	XIN Hairui, HAO Yue, ZHANG Chuang, GU Xianhong. Research Advance in the Regulation Mechanism of Glucose-regulated Protein 94 in Hepatocyte Apoptosis Induced by Endoplasmic Reticulum Stress [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 1963-1971.
[15]	WANG Bin, YIN Shi, XIONG Xianrong, QIN Wenchang, HUANG Xiangyue, LI Jian. Cloning of Yak HDAC1 Gene and Its Expression Pattern in Tissues and Oocyte during the Process of Meiosis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 1997-2004.

Study on the Relationship between Cumulus Granulose Cells Apoptosis and Oocyte Development in Buffalo

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments