Abstract:

One virus strain was isolated from the suspicious respiratory tract disease samples of nasal secretion of an invasive bovine from a beef farm in Heilongjiang Province, then it was systematically identified by a series of methods. The results indicated that the virus could grow well, propagate and induce typical cytopathic effect with syncytium formation in Vero cells. The isolate was insensitive to 5iodo2deoxyuridine, but sensitive to acid, chloroform and ether. The virus was not resistant to heat, and could be inactivated, which exposed at 56 ℃ for 30 min. The isolate could not agglutinate and adsorb erythrocytes of human, some animals and chicken. The virus could be neutralized by bovine respiratory syncytial virus (BRSV) standard positive serum. The RNA extracted from the cell culture of the isolated virus was subjected to RTPCR analysis, utilizing a pair of specific primers, and the sequence consisting of 596 bp lying in the gene encoding the nucleocapsid protein was amplified. The nucleotide identity of the fragments of the N genes between BRSV strains in GenBank database and the isolates ranged from 97.8％ to 99.3％. Above results showed that the isolated virus was bovine respiratory syncytial virus, and it was named BRSV HJ strain.

Key words: bovine respiratory syncytial virus, isolation, identification