一株牦牛源G6P[11]型牛轮状病毒的分离鉴定及部分基因的序列分析

doi:10.11843/j.issn.0366-6964.2019.03.014

Abstract

Abstract:

The aim of this study was to isolate and identify bovine rotavirus (BRV) from the diarrhea stool samples of Yak. BRV-positive samples detected by RT-PCR was used to inoculate into MA-104 cells for virus isolation and identification, and the complete genes of VP4, VP6 and VP7 were sequenced to determine their molecular characteristics. The cytopathic effect occurred after three continuous blind passages, and the stable time that cytopathic effect occurred was found after seven passages. The virus titer was 10^8.39TCID₅₀·mL^-1 after plaque purification. The isolate, named HY-1 strain, was confirmed to be BRV by RT-PCR, indirect immunofluorescence and electron microscopy. The complete VP4, VP6 and VP7 genes of HY-1 strain were sequenced successfully, showing that HY-1 strain belong to G6P[11] I2 genotype. The phylogenetic analysis showed that the complete VP4, VP6 and VP7 segments of HY-1 strain were closely genetic related to Chinese bovine DQ-75 strain and Indian bovine M-1 and RUBV319 strains, respectively, which indicated that HY-1 strain may be a reassortant strain. Compared with the domestic G6 and P[11] BRV strains, VP4 and VP7 of HY-1 isolate had significant variation in the important amino acid regions. In this study, a BRV strain was successfully isolated from yak with G6P[11] genotype. To the best of our knowledge, this is the first report on the identification of G6P[11] BRV in China.

CLC Number:

S852.659.4

LI Ran, TANG Cheng, LUO Xue, YUE Hua. Isolation and Gene Sequence Analysis of G6P[11] Bovine Rotavirus HY-1 Strain from Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 592-601.

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Isolation and Gene Sequence Analysis of G6P[11] Bovine Rotavirus HY-1 Strain from Yak

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