Abstract: This study aimed to comprehensively identify a strain of Brucella (B. abortus), tentatively designated BJ1, to provide a reference strain for the research of deer brucellosis. B. abortus BJ1 was diluted and cultivated on TSA plate, and single colony was inoculated into thiopurine and basic fuchsin medium for dye sensitivity test; The TSA plates inoculated with B. abortus BJ1 were placed in a common biochemical incubator and CO₂ incubator for 72 h at 37℃ to observe its dependence on CO₂; B. abortus BJ1 was inoculated into lead acetate medium for H₂S release test; Bacterial S/R type was identified by crystal violet staining and thermal agglutination test; mono-specific serum reactivity was determined by plate agglutination test; bacterial species were identified by AMOS-PCR. Guinea pigs were infected with 1×10⁹ CFU of BJ1 for 14 days to determine the virulence of BJ1 by measuring the number of B. abortus in one gram of spleen of the infected guinea pig. The entire genome was sequenced by next-generation sequencing approach, and phylogenetic analysis was performed using Mauve software to compare with B. abortus 2308. Results were as follows:B. abortus BJ1 was demonstrated to grow independent of CO₂, and produced H₂S. BJ1 was able to grow on medium containing thiopurine and basic fuchsin, and it was confirmed to be smooth phenotype with capability of agglutination with M factor serum. A number of 1.17×10⁶- 2.05×10⁶ CFU of the strain could be obtained from one gram of spleen 14 days post infection. The genome size of BJ1 was 3 270 584 bp, and a total of 46 Indel difference were found in the coding region of BJ1 genome, compared to the B. abortus 2308 strain. Phylogenetic analysis showed that BJ1 exhibited high homology with B. abortus 8416. B. abortus BJ1 was identified to be a bovine species type 9 and enriched the Brucella species. Our findings provide a reference for in-depth understanding of the epidemiology of brucellosis in wild animals of China.