苏钟猪脂蛋白脂肪酶的组织表达谱及其生物信息学分析

Abstract

Abstract:

In order to understand the structure and function of porcine LPL gene, the mRNA expression profile of porcine LPL in five tissues (heart, liver, kidney, subcutaneous fat and longissimus muscle) were examined in Suzhong pigs by Real-time PCR. Besides, the coding sequences (CDS) of LPL were cloned and sequenced in Suzhong pigs, and molecular structure and function of LPL were analyzed by bioinformatics methods. The results showed that mRNA expression abundance of LPL in different tissues tended to be subcutaneous fat > heart>kidney>longissimus muscle>liver. Among these tissues, LPL expression in subcutaneous fat was significantly higher than that in any other tissues (P<0.01), and expression in longissimus muscle of boars was significantly higher than that of sows (P<0.01). There was significant difference of eye-muscle area and lean-meat percentage traits between boars and sows(P<0.05). Bioinformatic analysis revealed that porcine LPL gene encode a protein of 479 amino acids, and its theoretical isoelectric point was 4.99. LPL protein was a hydrophobic and secreted protein with a signal peptide. In addition, Suzhong pig was closed to cattle, sheep and cat in phylogenetic tree. These findings suggest that the LPL may play an important role in regulating fat deposition in tissues of Suzhong pigs, such as subcutaneous fat, heart, kidney and muscle, etc, and it can be an important candidate gene for improving meat quality in Suzhong pigs.

CLC Number:

S813.3
S828

FU Yan-feng, LI Bi-xia, LI Lan, FANG Xiao-min, WANG Xue-min, REN Shou-wen. Expression Profile and Bioinformatics Analysis of Lipoprotein Lipase in Suzhong Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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References

［1］VAN WIJK H, ARTS D, MATTHEWS J, et al. Genetic parameters for carcass composition and pork quality estimated in a commercial production chain［J］. J Anim Sci, 2005, 83: 324-333.

［2］VAN WIJK H, DIBBITS B, BARON E, et al. Identification of quantitative trait loci for carcass composition and pork quality traits in a commercial finishing cross［J］. J Anim Sci, 2006, 84: 789-799.

［3］葛云山. 苏钟猪的选育［J］. 养猪, 2003,6: 19-21.

［4］TADDEI I, MORISHIMA M, HUYNH T. et al. Genetic factors are major determinants of phenotypic variability in a mouse model of the DiGeorge/del22q11 syndromes［J］. Proc Natl Acad Sci, 2001, 98: 11428-11431.

［5］WATSON NF, GOLDBERG J, ARGUELLES L, et al. Genetic and environmental influences on insomnia, daytime sleepiness, and obesity in twins［J］. Sleep, 2006, 29(5): 645-649.

［6］PRIOR S J, ROTH S M, WANG X, et al. Genetic and environmental influences on skeletal muscle phenotypes as a function of age and sex in large, multigenerational families of African heritage［J］. J Appl Physiol, 2007, 103: 1121-1127.

［7］杨海玲, 曾勇庆, 魏述东, 等. 莱芜猪脂肪代谢酶活性的发育性变化及其对肌内脂肪沉积的影响［J］. 畜牧兽医学报, 2005, 36: 1150-1154.

［8］HAHN P F. Abolishment of alimentary lipemia following injection of heparin［J］. Science (New York, NY), 1943, 98: 19-20.

［9］SCANU A. Serum high-density lipoprotein: effect of change in structure on activity of chicken adipose tissue lipase［J］. Science, 1966, 153: 640-641.

［10］HARBITZ I, KRISTENSEN T, KRAN S, et al. Isolation and sequencing of porcine lipoprotein lipase cDNA and its use in multiallelic restriction fragment length polymorphism detection［J］. Anim Genet, 1992, 23: 517-522.

［11］SHEN Y, LOOKENE A, ZHANG L, et al. Site-directed mutagenesis of apolipoprotein CII to probe the role of its secondary structure for activation of lipoprotein lipase［J］. J Biol Chem, 2010, 285: 7484-7492.

［12］SAGOO G S, TATT I, SALANTI G, et al. Seven lipoprotein lipase gene polymorphisms, lipid fractions, and coronary disease: a HuGE association review and meta-analysis［J］. Am J Epidemiol, 2008, 168: 1233-1246.

［13］LIVAK K, SCHMITTGEN T. Analysis of relative gene expression data using real-time quantitative PCR and the 2-［Delta］［Delta］ CT method［J］. Methods, 2001, 25: 402-408.

［14］SCHMITTGEN T, LIVAK K. Analyzing real-time PCR data by the comparative CT method［J］. Nat Protocols, 2008, 3: 1101-1108.

［15］钱源. 莱芜猪PID1基因的功能分析及表达谱研究［J］. 畜牧兽医学报, 2011, 42(5): 621-628.

［16］邢成锋. 脂蛋白脂肪酶基因的研究进展［J］. 华北农学报, 2008, 23: 84-87.

［17］胡代奎, 肖永祚. 雅南猪及其杂交种血液蛋白多态与胴体性状及肉质间相关性研究［J］. 四川农业大学学报, 1994, 12: 263-270.

［18］魏彩虹. 猪脂肪的细胞形态学与脂肪代谢酶的研究［J］. 中国畜牧杂志, 2002, 38: 28-29.

［19］MEAD J R, IRVINE S A, RAMJI D P. Lipoprotein lipase: structure, function, regulation, and role in disease［J］. J Mol Med, 2002, 80: 753-769.

［20］杨献光. 哺乳动物脂蛋白脂肪酶基因的进化研究［J］. 生物技术通报, 2010, 10: 139-142.

［21］张振波. 猪脂蛋白脂酶 (LPL) 和激素敏感脂肪酶 (HSL) 基因的序列和多态性分析及体外表达的研究［D］. 武汉: 华中农业大学, 2005.

［22］ARAU／JO L, CENDOROGLO M, GIGEK C, et al. Association of lipase lipoprotein polymorphisms with high-density lipoprotein and triglycerides in elderly men［J］. Genet Mol Res, 2010, 9: 89-96.

［23］PULINILKUNNIL T, RODRIGUES B. Cardiac lipoprotein lipase: metabolic basis for diabetic heart disease［J］. Cardiovascular Res, 2006, 69: 329-340.

［24］SALAH A, KHAN M, ESMAIL N, et al. Genetic polymorphism of S447X lipoprotein lipase (LPL) and the susceptibility to hypertension［J］. J Critical Care, 2009, 24: e11-e14.

［25］WANG H, ECKEL R H. Lipoprotein lipase: from gene to obesity［J］. Am J Physiol-Endocrinol Metabo, 2009, 297: e271-e288.

［26］MADSEN M B, BIRCK M M, FREDHOLM M, et al. Expression studies of the obesity candidate gene FTO in pig［J］. Anim Biotechnol, 2009, 21: 51-63.

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Expression Profile and Bioinformatics Analysis of Lipoprotein Lipase in Suzhong Pigs

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