部分地区奶牛腹泻粪便样中诺如病毒的检测和演化分析

doi:10.11843/j.issn.0366-6964.2019.05.015

Abstract

Abstract:

Bovine norovirus (BNoV) is a pathogen causing calf diarrhea and has been detected in 19 countries. The aims of this study were to investigate the prevalence and molecular characteristics of the virus in several regions of China. In this study, a total of 93 diarrheic fecal samples and 54 healthy fecal samples collected from 12 farms in 5 provinces were detected by RT-PCR. The results showed that the detection rate of BNoV in diarrhea samples (25.81%) was significantly higher than that of the healthy samples (9.26%) (P<0.01), which proved that the virus was associated with calf diarrhea. Phylogenetic analysis based on 29 RdRp sequences (partial polymerase sequences) showed that 5 strains clustered into GⅢ.1 and the remaining 24 strains clustered into GⅢ.2. Phylogenetic analysis based on 18 partial VP1 and 13 VP2 sequences showed that BNoV strains in China present a unique evolutionary. These results indicated that, as an emerging pathogen with unique evolutionary characteristics in China, BNoV has been widely circulated among dairy calves, providing an important reference for the prevention and control of calf diarrhea in China.

CLC Number:

S852.659.6

WANG Yuelin, GUO Zijing, YUE Hua, TANG Cheng. Detection and Evolutionary Analysis of Bovine Noroviruses in Diarrheic Fecals of Dairy Calves in Some Areas[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1048-1055.

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Detection and Evolutionary Analysis of Bovine Noroviruses in Diarrheic Fecals of Dairy Calves in Some Areas

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