疏肝益阳胶囊对炔雌醚诱导大鼠生精紊乱和氧化应激的缓解作用

doi:10.11843/j.issn.0366-6964.2019.02.021

Abstract

Abstract:

The study was aimed to evaluate the probable protective effect of Shugan Yiyang Capsule (SGYY, Chinese herbal extract with highly efficient antioxidant activities) against testicular toxicity and oxidative stress in adult male rats exposed to quinestrol (QES) and study the possible mechanisms underlying these effects. Twenty 8-week-old male SD rats were randomly divided into 4 groups. After 1 week of adaptation, the rats were treated with gavage once daily for 2 weeks. Control group:0.1 mL physiological saline+0.1 mL olive oil; SGYY group:100 mg·kg^-1 SGYY; QES group:0.1 mg·kg^-1 QES; QES+SGYY group:0.1 mg·kg^-1 QES+100 mg·kg^-1 SGYY. QES was dissolved in olive oil; SGYY was dissolved in physiological saline. After the treatments, the testis, epididymis, seminal vesicle and prostate were weighed, and the long and short diameters were measured. The changes in the histological structure of seminiferous tubules and the proportion of spermatogenic cells were observed in testicular tissue sections by HE staining. The expression of PCNA in spermatogenic cells of testis was detected by immunohistochemistry, and the apoptosis was detected by Tunel. The blood plasma was separated and the content of testosterone was detected. The antioxidant enzyme activity was detected in the testicular homogenate. The results showed that exposure to QES decreased the weights of reproductive organs and the sperms counts in cauda epididymis of rats. A significant decrease in the seminiferous tubular area, tubular diameter, height of germinal epithelium, number of spermatogenic cell (per seminiferous tubules) and expression of PCNA level with a significant increase in the Tunel levels were observed in the testis of experimental rats over the controls. These events were accompanied by a significant reduction in the plasma testosterone levels in QES exposed rats, indicating reduced steroidogenesis. Significant reduction in the activities of SOD, GSH-Px and T-AOC and a significant increase in MDA were also observed in the testis of QES exposed rats over the controls. Conversely, supplementation of SGYY (100 mg·kg^-1) ameliorated the male reproductive health in rats exposed to QES as evidenced by increased reproductive organ weights, sperm counts, testicular steroidogenesis, spermatogenic cell proliferation and testicular antioxidants whereas MDA and Tunel levels decreased. To sum up, the results show that SGYY increased the number of spermatogenic cells and improved spermatogenesis in the testis by improving antioxidant function, inhibiting oxidative stress and promoting testosterone secretion.

CLC Number:

S853.9

LI Jian, CHEN Yaoxing, CHEN Funing, WANG Zixu, CAO Jing, DONG Yulan, LIU Yang, WANG Xingde, WANG Yang. Protective Effect of Shugan Yiyang Capsule against Quinestrol-induced Testicular Disturbances and Oxidative Stress in Male Rats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 422-430.

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Protective Effect of Shugan Yiyang Capsule against Quinestrol-induced Testicular Disturbances and Oxidative Stress in Male Rats

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